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Article

B7-H3 silencing inhibits tumor progression of mantle cell lymphoma and enhances chemosensitivity

  • Authors:
    • Wei Zhang
    • Yanfang Wang
    • Jing Wang
    • Fei Dong
    • Mingxia Zhu
    • Wenli Wan
    • Haishen Li
    • Feifei Wu
    • Xinxing Yan
    • Xiaoyan Ke
  • View Affiliations / Copyright

    Affiliations: Department of Hematology and Lymphoma Research Center, Peking University Third Hospital, Beijing 100191, P.R. China
  • Pages: 2562-2572
    |
    Published online on: April 9, 2015
       https://doi.org/10.3892/ijo.2015.2962
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Abstract

B7-H3 (CD276), known as a member of B7 immunoregulatory family, is a type I transmembrane glycoprotein aberrantly expressed in numerous types of cancer and associated with poor prognosis. However, the role of B7-H3 in oncogenesis and chemosensitivity of mantle cell lymphoma (MCL) remains unknown. We determined the effects of downregulating B7-H3 expression on tumor progression and the sensitivity of chemotherapeutic drug in mantle cell lymphoma. B7-H3 knockdown was performed using lentivirus transduction in the Maver and Z138 mantle cell lymphoma cell lines, respectively. The effects of B7-H3 on cell proliferation, cycle, migration and invasion were investigated by CCK-8 assay, methyl cellulose colony forming assay, PI staining, and Transwell assays in vitro. By establishing Maver and Z138 xenograft models, the effects of B7-H3 on tumorigenicity were observed, and Ki-67 and PCNA was detected by immunohistochemistry. The downregulation of B7-H3 significantly decreased tumor proliferation in MCL in vitro and in vivo. In the B7-H3 knockdown groups of Maver and Z138 xenograft models, the mean inhibition rate of tumor growth was 59.1 and 65.0% (p=0.010 and 0.003), and the expression of both Ki-67 and PCNA were significantly lower, respectively. After B7-H3 silencing, the cell cycles of Maver and Z138 were both arrested at G0/G1 phase, and the cell migration rates and invasion capacity were decreased as well. Moreover, the impacts of B7-H3 RNAi on the antitumor effect of chemotherapy drugs were determined with CCK-8 and Annexin V-FITC/PI assays in vitro and with xenograft models in vivo. The silencing of B7-H3 increased the sensitivity of Maver and Z138 cells to rituximab and bendamustine and enhanced the drug-induced apoptosis, respectively. Our study demonstrates for the first time that B7-H3 promotes mantle cell lymphoma progression and B7-H3 knockdown significantly enhances the chemosensitivity. This may provide a new therapeutic approach to mantle cell lymphoma.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang W, Wang Y, Wang J, Dong F, Zhu M, Wan W, Li H, Wu F, Yan X, Ke X, Ke X, et al: B7-H3 silencing inhibits tumor progression of mantle cell lymphoma and enhances chemosensitivity. Int J Oncol 46: 2562-2572, 2015.
APA
Zhang, W., Wang, Y., Wang, J., Dong, F., Zhu, M., Wan, W. ... Ke, X. (2015). B7-H3 silencing inhibits tumor progression of mantle cell lymphoma and enhances chemosensitivity. International Journal of Oncology, 46, 2562-2572. https://doi.org/10.3892/ijo.2015.2962
MLA
Zhang, W., Wang, Y., Wang, J., Dong, F., Zhu, M., Wan, W., Li, H., Wu, F., Yan, X., Ke, X."B7-H3 silencing inhibits tumor progression of mantle cell lymphoma and enhances chemosensitivity". International Journal of Oncology 46.6 (2015): 2562-2572.
Chicago
Zhang, W., Wang, Y., Wang, J., Dong, F., Zhu, M., Wan, W., Li, H., Wu, F., Yan, X., Ke, X."B7-H3 silencing inhibits tumor progression of mantle cell lymphoma and enhances chemosensitivity". International Journal of Oncology 46, no. 6 (2015): 2562-2572. https://doi.org/10.3892/ijo.2015.2962
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang W, Wang Y, Wang J, Dong F, Zhu M, Wan W, Li H, Wu F, Yan X, Ke X, Ke X, et al: B7-H3 silencing inhibits tumor progression of mantle cell lymphoma and enhances chemosensitivity. Int J Oncol 46: 2562-2572, 2015.
APA
Zhang, W., Wang, Y., Wang, J., Dong, F., Zhu, M., Wan, W. ... Ke, X. (2015). B7-H3 silencing inhibits tumor progression of mantle cell lymphoma and enhances chemosensitivity. International Journal of Oncology, 46, 2562-2572. https://doi.org/10.3892/ijo.2015.2962
MLA
Zhang, W., Wang, Y., Wang, J., Dong, F., Zhu, M., Wan, W., Li, H., Wu, F., Yan, X., Ke, X."B7-H3 silencing inhibits tumor progression of mantle cell lymphoma and enhances chemosensitivity". International Journal of Oncology 46.6 (2015): 2562-2572.
Chicago
Zhang, W., Wang, Y., Wang, J., Dong, F., Zhu, M., Wan, W., Li, H., Wu, F., Yan, X., Ke, X."B7-H3 silencing inhibits tumor progression of mantle cell lymphoma and enhances chemosensitivity". International Journal of Oncology 46, no. 6 (2015): 2562-2572. https://doi.org/10.3892/ijo.2015.2962
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