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Article

The flavonoid morin from Moraceae induces apoptosis by modulation of Bcl-2 family members and Fas receptor in HCT 116 cells

  • Authors:
    • Hwang-Bo Hyun
    • Won Sup Lee
    • Se-Il Go
    • Arulkumar Nagappan
    • Cheol Park
    • Min Ho Han
    • Su Hyun Hong
    • Gonsup Kim
    • Gi Young Kim
    • Jaehun Cheong
    • Chung Ho Ryu
    • Sung Chul Shin
    • Yung Hyun Choi
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry, Dongeui University College of Korean Medicine, Busan 614-052, Republic of Korea, Department of Internal Medicine, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 660-702, Republic of Korea, Department of Molecular Biology, College of Natural Sciences, Dongeui University, Busan 614-714, Republic of Korea, Department of Biochemistry, Dongeui University College of Oriental Medicine, Busan 614-052, Republic of Korea, School of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea, Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea, Department of Molecular Biology, College of Natural Sciences, Pusan National University, Busan 609-735, Republic of Korea, Division of Applied Life Science (BK 21 Program), Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea, Department of Chemistry, Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea
  • Pages: 2670-2678
    |
    Published online on: April 16, 2015
       https://doi.org/10.3892/ijo.2015.2967
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Abstract

It is evident based on literature that flavonoids from fruit can safely modulate cancer cell biology and induce apoptosis. Therefore, we investigated the anticancer activity of morin, a flavonoid which is plentiful in twigs of mulberry focusing on apoptosis, and its mechanisms. Morin upregulated the Fas receptor, and activates caspase-8, -9 and -3 in HCT-116 cells. Morin also activates Bid, and induced the loss of mitochondrial membrane potential (MMP, ∆Ψm) with Bax protein activation and cytochrome c release. In addition, morin induced ROS generation which was not blocked by N-acetylcysteine. Morin also suppressed Bcl-2 and cIAP-1, anti-apoptotic proteins, which may contribute to augmentation of morin-triggered apoptosis. As an upstream signaling pathway, suppressed Akt activity by morin was associated to apoptosis. This study suggests that morin induces caspase-dependent apoptosis through extrinsic pathway by upregulating Fas receptor as well as through the intrinsic pathway by modulating Bcl-2 and IAP family members, and ROS generation, and that Akt is the critical upstream signaling that regulates the apoptotic effect of morin in human colon cancer HCT-116 cells.
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Copy and paste a formatted citation
Spandidos Publications style
Hyun H, Lee WS, Go S, Nagappan A, Park C, Han MH, Hong SH, Kim G, Kim GY, Cheong J, Cheong J, et al: The flavonoid morin from Moraceae induces apoptosis by modulation of Bcl-2 family members and Fas receptor in HCT 116 cells. Int J Oncol 46: 2670-2678, 2015.
APA
Hyun, H., Lee, W.S., Go, S., Nagappan, A., Park, C., Han, M.H. ... Choi, Y.H. (2015). The flavonoid morin from Moraceae induces apoptosis by modulation of Bcl-2 family members and Fas receptor in HCT 116 cells. International Journal of Oncology, 46, 2670-2678. https://doi.org/10.3892/ijo.2015.2967
MLA
Hyun, H., Lee, W. S., Go, S., Nagappan, A., Park, C., Han, M. H., Hong, S. H., Kim, G., Kim, G. Y., Cheong, J., Ryu, C. H., Shin, S. C., Choi, Y. H."The flavonoid morin from Moraceae induces apoptosis by modulation of Bcl-2 family members and Fas receptor in HCT 116 cells". International Journal of Oncology 46.6 (2015): 2670-2678.
Chicago
Hyun, H., Lee, W. S., Go, S., Nagappan, A., Park, C., Han, M. H., Hong, S. H., Kim, G., Kim, G. Y., Cheong, J., Ryu, C. H., Shin, S. C., Choi, Y. H."The flavonoid morin from Moraceae induces apoptosis by modulation of Bcl-2 family members and Fas receptor in HCT 116 cells". International Journal of Oncology 46, no. 6 (2015): 2670-2678. https://doi.org/10.3892/ijo.2015.2967
Copy and paste a formatted citation
x
Spandidos Publications style
Hyun H, Lee WS, Go S, Nagappan A, Park C, Han MH, Hong SH, Kim G, Kim GY, Cheong J, Cheong J, et al: The flavonoid morin from Moraceae induces apoptosis by modulation of Bcl-2 family members and Fas receptor in HCT 116 cells. Int J Oncol 46: 2670-2678, 2015.
APA
Hyun, H., Lee, W.S., Go, S., Nagappan, A., Park, C., Han, M.H. ... Choi, Y.H. (2015). The flavonoid morin from Moraceae induces apoptosis by modulation of Bcl-2 family members and Fas receptor in HCT 116 cells. International Journal of Oncology, 46, 2670-2678. https://doi.org/10.3892/ijo.2015.2967
MLA
Hyun, H., Lee, W. S., Go, S., Nagappan, A., Park, C., Han, M. H., Hong, S. H., Kim, G., Kim, G. Y., Cheong, J., Ryu, C. H., Shin, S. C., Choi, Y. H."The flavonoid morin from Moraceae induces apoptosis by modulation of Bcl-2 family members and Fas receptor in HCT 116 cells". International Journal of Oncology 46.6 (2015): 2670-2678.
Chicago
Hyun, H., Lee, W. S., Go, S., Nagappan, A., Park, C., Han, M. H., Hong, S. H., Kim, G., Kim, G. Y., Cheong, J., Ryu, C. H., Shin, S. C., Choi, Y. H."The flavonoid morin from Moraceae induces apoptosis by modulation of Bcl-2 family members and Fas receptor in HCT 116 cells". International Journal of Oncology 46, no. 6 (2015): 2670-2678. https://doi.org/10.3892/ijo.2015.2967
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