MicroRNA-33b inhibits lung adenocarcinoma cell growth, invasion, and epithelial-mesenchymal transition by suppressing Wnt/β-catenin/ZEB1 signaling

  • Authors:
    • Jingjing Qu
    • Min Li
    • Jian An
    • Bingrong Zhao
    • Wen Zhong
    • Qihua Gu
    • Liming Cao
    • Huaping Yang
    • Chengping Hu
  • View Affiliations

  • Published online on: September 28, 2015     https://doi.org/10.3892/ijo.2015.3187
  • Pages: 2141-2152
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Abstract

Altered expression of microRNA (miRNA) is associated with lung carcinogenesis and metastasis. Our previous study of lung cancer miRNAs using the gene chip assay demonstrated altered miR-33b expression in lung adenocarcinoma. The present study further investigated miR-33b expression, function, and gene regulation in lung cancer cells in vitro and in nude mouse xenografts. Our data showed that the level of miR-33b expression was dramatically decreased in lung adenocarcinoma cell lines and tissues and that the reduced miR-33b expression was associated with tumor lymph node metastasis. Furthermore, restoration of miR-33b expression inhibited lung adenocarcinoma cell proliferation, migration, and invasion and tumor cell epithelial-mesenchymal transition (EMT) in vitro. Luciferase assay revealed that miR-33b bound to ZEB1 3'-UTR region and inhibited ZEB1 expression, while expression of ZEB1 mRNA and miR-33b was inversely associated with lung adenocarcinoma cell lines and tissues. Subsequently, we found that miR-33b suppressed the activity of WNT/β-catenin signaling in lung adenocarcinoma cells and in turn suppressed tumor cell growth and EMT in vitro and in vivo nude mouse xenografts. In conclusion, the present study provided novel insight into the molecular mechanism of lung adenocarcinoma progression. MicroRNA-33b should be further investigated as a potential therapeutic target in human lung adenocarcinoma.
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December-2015
Volume 47 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Qu J, Li M, An J, Zhao B, Zhong W, Gu Q, Cao L, Yang H and Hu C: MicroRNA-33b inhibits lung adenocarcinoma cell growth, invasion, and epithelial-mesenchymal transition by suppressing Wnt/β-catenin/ZEB1 signaling. Int J Oncol 47: 2141-2152, 2015
APA
Qu, J., Li, M., An, J., Zhao, B., Zhong, W., Gu, Q. ... Hu, C. (2015). MicroRNA-33b inhibits lung adenocarcinoma cell growth, invasion, and epithelial-mesenchymal transition by suppressing Wnt/β-catenin/ZEB1 signaling. International Journal of Oncology, 47, 2141-2152. https://doi.org/10.3892/ijo.2015.3187
MLA
Qu, J., Li, M., An, J., Zhao, B., Zhong, W., Gu, Q., Cao, L., Yang, H., Hu, C."MicroRNA-33b inhibits lung adenocarcinoma cell growth, invasion, and epithelial-mesenchymal transition by suppressing Wnt/β-catenin/ZEB1 signaling". International Journal of Oncology 47.6 (2015): 2141-2152.
Chicago
Qu, J., Li, M., An, J., Zhao, B., Zhong, W., Gu, Q., Cao, L., Yang, H., Hu, C."MicroRNA-33b inhibits lung adenocarcinoma cell growth, invasion, and epithelial-mesenchymal transition by suppressing Wnt/β-catenin/ZEB1 signaling". International Journal of Oncology 47, no. 6 (2015): 2141-2152. https://doi.org/10.3892/ijo.2015.3187