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Loss of autophagy-related protein Beclin 1 may define poor prognosis in ovarian clear cell carcinomas

  • Authors:
    • Hiroshi Katagiri
    • Kentaro Nakayama
    • Sultana Razia
    • Kohei Nakamura
    • Emi Sato
    • Tomoka Ishibashi
    • Masako Ishikawa
    • Kouji Iida
    • Noriyoshi Ishikawa
    • Yoshiro Otsuki
    • Satoru Nakayama
    • Satoru Kyo
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Shimane University School of Medicine, Izumo 693-8501, Japan, Department of Organ Pathology, Shimane University School of Medicine, Izumo 693-8501, Japan, Department of Pathology, Seirei Hamamatsu General Hospital, Hamamatsu 430-8558, Japan, Department of Obstetrics and Gynecology, Seirei Hamamatsu General Hospital, Hamamatsu 430-8558, Japan
    Copyright: © Katagiri et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2037-2044
    |
    Published online on: October 6, 2015
       https://doi.org/10.3892/ijo.2015.3191
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Abstract

The aim of the present study was to clarify the role of autophagy in cisplatin (CDDP) sensitivity in OCCCs and the role of Beclin 1 in OCCC progression. Autophagy was measured using: i) western blot analysis of LC3 and p62 and ii) microscopic observation of GFP-LC3 puncta. Autophagy was suppressed using chloroquine and Beclin 1 siRNA. Surgical specimens were examined for Beclin 1 protein expression by immunohistochemistry. The correlations between the loss of Beclin 1 expression and clinicopathological characteristics, prognosis and chemosensitivity were investigated. Inhibition of autophagy by chloroquine or Beclin 1 siRNA did not enhance the sensitivity of the ES2 and TOV-21G OCCC cell lines to CDDP. Loss of Beclin 1 expression was observed in 38.3% (23/60) of the analyzed tumors. There was no significant correlation between loss of Beclin 1 expression and FIGO stage, CA125 levels, patient age, status of endometriosis, Ki-67 labeling index, chemotherapy regimen or status of residual tumor. However, negative expression of Beclin 1 was associated with a shorter progression-free survival in comparison to positive Beclin 1 expression in OCCC who received cytoreductive surgery, followed by a standard platinum-based chemotherapy regimen (P=0.027, log-rank test). Beclin 1-negative tumors were no more resistant to primary adjuvant chemotherapy than were Beclin 1-positive tumors (50.0 vs. 66.7%, P=0.937). Beclin 1 knockdown using siRNA increased cell growth but not cell migration and invasion in ES2 and TOV-21G OCCC cell lines. Autophagy defects caused by loss of Beclin 1 are not related to chemoresistance and metastasis, but may be associated with malignant phenotype and poor prognosis of OCCC.
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Copy and paste a formatted citation
Spandidos Publications style
Katagiri H, Nakayama K, Razia S, Nakamura K, Sato E, Ishibashi T, Ishikawa M, Iida K, Ishikawa N, Otsuki Y, Otsuki Y, et al: Loss of autophagy-related protein Beclin 1 may define poor prognosis in ovarian clear cell carcinomas. Int J Oncol 47: 2037-2044, 2015.
APA
Katagiri, H., Nakayama, K., Razia, S., Nakamura, K., Sato, E., Ishibashi, T. ... Kyo, S. (2015). Loss of autophagy-related protein Beclin 1 may define poor prognosis in ovarian clear cell carcinomas. International Journal of Oncology, 47, 2037-2044. https://doi.org/10.3892/ijo.2015.3191
MLA
Katagiri, H., Nakayama, K., Razia, S., Nakamura, K., Sato, E., Ishibashi, T., Ishikawa, M., Iida, K., Ishikawa, N., Otsuki, Y., Nakayama, S., Kyo, S."Loss of autophagy-related protein Beclin 1 may define poor prognosis in ovarian clear cell carcinomas". International Journal of Oncology 47.6 (2015): 2037-2044.
Chicago
Katagiri, H., Nakayama, K., Razia, S., Nakamura, K., Sato, E., Ishibashi, T., Ishikawa, M., Iida, K., Ishikawa, N., Otsuki, Y., Nakayama, S., Kyo, S."Loss of autophagy-related protein Beclin 1 may define poor prognosis in ovarian clear cell carcinomas". International Journal of Oncology 47, no. 6 (2015): 2037-2044. https://doi.org/10.3892/ijo.2015.3191
Copy and paste a formatted citation
x
Spandidos Publications style
Katagiri H, Nakayama K, Razia S, Nakamura K, Sato E, Ishibashi T, Ishikawa M, Iida K, Ishikawa N, Otsuki Y, Otsuki Y, et al: Loss of autophagy-related protein Beclin 1 may define poor prognosis in ovarian clear cell carcinomas. Int J Oncol 47: 2037-2044, 2015.
APA
Katagiri, H., Nakayama, K., Razia, S., Nakamura, K., Sato, E., Ishibashi, T. ... Kyo, S. (2015). Loss of autophagy-related protein Beclin 1 may define poor prognosis in ovarian clear cell carcinomas. International Journal of Oncology, 47, 2037-2044. https://doi.org/10.3892/ijo.2015.3191
MLA
Katagiri, H., Nakayama, K., Razia, S., Nakamura, K., Sato, E., Ishibashi, T., Ishikawa, M., Iida, K., Ishikawa, N., Otsuki, Y., Nakayama, S., Kyo, S."Loss of autophagy-related protein Beclin 1 may define poor prognosis in ovarian clear cell carcinomas". International Journal of Oncology 47.6 (2015): 2037-2044.
Chicago
Katagiri, H., Nakayama, K., Razia, S., Nakamura, K., Sato, E., Ishibashi, T., Ishikawa, M., Iida, K., Ishikawa, N., Otsuki, Y., Nakayama, S., Kyo, S."Loss of autophagy-related protein Beclin 1 may define poor prognosis in ovarian clear cell carcinomas". International Journal of Oncology 47, no. 6 (2015): 2037-2044. https://doi.org/10.3892/ijo.2015.3191
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