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Clinical significance of expanded Foxp3+ Helios- regulatory T cells in patients with non-small cell lung cancer

  • Authors:
    • Satoshi Muto
    • Yuki Owada
    • Takuya Inoue
    • Yuzuru Watanabe
    • Takumi Yamaura
    • Mitsuro Fukuhara
    • Naoyuki Okabe
    • Yuki Matsumura
    • Takeo Hasegawa
    • Jun Osugi
    • Mika Hoshino
    • Mitsunori Higuchi
    • Hiroyuki Suzuki
    • Mitsukazu Gotoh
  • View Affiliations / Copyright

    Affiliations: Department of Regenerative Surgery, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan
    Copyright: © Muto et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2082-2090
    |
    Published online on: October 12, 2015
       https://doi.org/10.3892/ijo.2015.3196
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Abstract

The functions of different regulatory T cell (Treg) types in cancer progression are unclear. Recently, expression of the transcription factor Helios was proposed as a marker for natural (non-induced) Tregs. The present study investigated the clinical significance of Helios expression in patients with non-small cell lung cancer (NSCLC). We enrolled 64 patients with NSCLC, of whom 45 were treated surgically and 19 received chemotherapy because of advanced/recurrent disease. Their peripheral blood mononuclear cells were examined by flow cytometry. From the 45 surgery patients, we matched 9 patients with recurrent disease with 9 stage-matched patients without recurrence (n=18), compared their specimens immunohistochemically for tumor infiltrating lymphocytes (TILs) and analyzed these data against clinicopathological factors. Helios expression in Foxp3+ Tregs was 47.5±13.3% in peripheral blood and 18.1±13.4% in tumor specimens. Percentage of Helios- Tregs among CD4+ T cells were significantly higher in the cancer patients (2.4%), especially those with stage IA disease (2.6%) than in healthy donors (1.5%; P<0.001). Patients with low levels of Helios expression in Tregs among their TILs had significantly poorer survival (P=0.038). Helios- Tregs may affect immune suppression, even in early stage NSCLC; they could also be a useful prognostic biomarker in patients with NSCLC, and possibly a novel cancer immunotherapy target.
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Copy and paste a formatted citation
Spandidos Publications style
Muto S, Owada Y, Inoue T, Watanabe Y, Yamaura T, Fukuhara M, Okabe N, Matsumura Y, Hasegawa T, Osugi J, Osugi J, et al: Clinical significance of expanded Foxp3+ Helios- regulatory T cells in patients with non-small cell lung cancer. Int J Oncol 47: 2082-2090, 2015.
APA
Muto, S., Owada, Y., Inoue, T., Watanabe, Y., Yamaura, T., Fukuhara, M. ... Gotoh, M. (2015). Clinical significance of expanded Foxp3+ Helios- regulatory T cells in patients with non-small cell lung cancer. International Journal of Oncology, 47, 2082-2090. https://doi.org/10.3892/ijo.2015.3196
MLA
Muto, S., Owada, Y., Inoue, T., Watanabe, Y., Yamaura, T., Fukuhara, M., Okabe, N., Matsumura, Y., Hasegawa, T., Osugi, J., Hoshino, M., Higuchi, M., Suzuki, H., Gotoh, M."Clinical significance of expanded Foxp3+ Helios- regulatory T cells in patients with non-small cell lung cancer". International Journal of Oncology 47.6 (2015): 2082-2090.
Chicago
Muto, S., Owada, Y., Inoue, T., Watanabe, Y., Yamaura, T., Fukuhara, M., Okabe, N., Matsumura, Y., Hasegawa, T., Osugi, J., Hoshino, M., Higuchi, M., Suzuki, H., Gotoh, M."Clinical significance of expanded Foxp3+ Helios- regulatory T cells in patients with non-small cell lung cancer". International Journal of Oncology 47, no. 6 (2015): 2082-2090. https://doi.org/10.3892/ijo.2015.3196
Copy and paste a formatted citation
x
Spandidos Publications style
Muto S, Owada Y, Inoue T, Watanabe Y, Yamaura T, Fukuhara M, Okabe N, Matsumura Y, Hasegawa T, Osugi J, Osugi J, et al: Clinical significance of expanded Foxp3+ Helios- regulatory T cells in patients with non-small cell lung cancer. Int J Oncol 47: 2082-2090, 2015.
APA
Muto, S., Owada, Y., Inoue, T., Watanabe, Y., Yamaura, T., Fukuhara, M. ... Gotoh, M. (2015). Clinical significance of expanded Foxp3+ Helios- regulatory T cells in patients with non-small cell lung cancer. International Journal of Oncology, 47, 2082-2090. https://doi.org/10.3892/ijo.2015.3196
MLA
Muto, S., Owada, Y., Inoue, T., Watanabe, Y., Yamaura, T., Fukuhara, M., Okabe, N., Matsumura, Y., Hasegawa, T., Osugi, J., Hoshino, M., Higuchi, M., Suzuki, H., Gotoh, M."Clinical significance of expanded Foxp3+ Helios- regulatory T cells in patients with non-small cell lung cancer". International Journal of Oncology 47.6 (2015): 2082-2090.
Chicago
Muto, S., Owada, Y., Inoue, T., Watanabe, Y., Yamaura, T., Fukuhara, M., Okabe, N., Matsumura, Y., Hasegawa, T., Osugi, J., Hoshino, M., Higuchi, M., Suzuki, H., Gotoh, M."Clinical significance of expanded Foxp3+ Helios- regulatory T cells in patients with non-small cell lung cancer". International Journal of Oncology 47, no. 6 (2015): 2082-2090. https://doi.org/10.3892/ijo.2015.3196
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