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Article

Downregulation of β3 integrin by miR-30a-5p modulates cell adhesion and invasion by interrupting Erk/Ets‑1 network in triple-negative breast cancer

Corrigendum in: /10.3892/ijo.2021.5169
  • Authors:
    • Wentong Li
    • Chuanliang Liu
    • Chunling Zhao
    • Limin Zhai
    • Shijun Lv
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Weifang Medical University, Weifang, Shandong 261053, P.R. China, The Third Department of Health Care, Weifang People's Hospital, Weifang, Shandong 261053, P.R. China, Department of Medical Biology, Weifang Medical University, Weifang, Shandong 261053, P.R. China
  • Pages: 1155-1164
    |
    Published online on: January 5, 2016
       https://doi.org/10.3892/ijo.2016.3319
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Abstract

Integrins are adhesion receptors involved in bidirectional signaling and are crucial for various cellular responses during normal homeostasis and pathological conditions, such as cancer progression and metastasis. In the present study, we demonstrated that blockage of β3 integrin-mediated cell- extracellular matrix interactions restrained triple-negative breast cancer (TNBC) growth, and elevated β3 integrin can trigger the rewiring of Erk/Ets-1 signaling pathways, thereby enhancing cell growth and invasion. Ectopic expression of miRNA has been implicated in the deregulation of integrin expression and activity, blocking of cancer tumor development and progression, and acquisition of metastatic phenotype. miR-30a-5p expression has been implicated in the progression of breast cancer. Overexpression of miR-30a-5p suppressed the proliferation, migration and invasion of breast cancer cells. On the contrary, inhibition of miR-30a-5p promoted the proliferation, migration, and invasion of TNBC cells by suppressing the expression of ERK/Ets-1 signal. An inverse correlation was found between the mRNA expressions of miR-30a-5p and β3 integrin in TNBC samples. Furthermore, bioinformatics analysis revealed the putative miR-30 binding sites in the 3'-UTR of β3 integrin. Results of luciferase assay revealed a strong repression of luciferase activity after transfection with miR‑30a-5p and wild-type 3'-UTR of β3 integrin. In TNBC cells, miR-30a-5p promoted an epithelial phenotype and suppressed invasion by specifically targeting β3 integrin subunit to subsequently interdict the β3 integrin/Erk/Ets-1 network.
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1 

Taylor MA, Davuluri G, Parvani JG, Schiemann BJ, Wendt MK, Plow EF, Schiemann WP and Sossey-Alaoui K: Upregulated WAVE3 expression is essential for TGF-β-mediated EMT and metastasis of triple-negative breast cancer cells. Breast Cancer Res Treat. 142:341–353. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Barcus CE, Keely PJ, Eliceiri KW and Schuler LA: Stiff collagen matrices increase tumorigenic prolactin signaling in breast cancer cells. J Biol Chem. 288:12722–12732. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Wang Y, Shenouda S, Baranwal S, Rathinam R, Jain P, Bao L, Hazari S, Dash S and Alahari SK: Integrin subunits alpha5 and alpha6 regulate cell cycle by modulating the chk1 and Rb/E2F pathways to affect breast cancer metastasis. Mol Cancer. 10:842011. View Article : Google Scholar : PubMed/NCBI

4 

Felding-Habermann B, O'Toole TE, Smith JW, Fransvea E, Ruggeri ZM, Ginsberg MH, Hughes PE, Pampori N, Shattil SJ, Saven A, et al: Integrin activation controls metastasis in human breast cancer. Proc Natl Acad Sci USA. 98:1853–1858. 2001. View Article : Google Scholar : PubMed/NCBI

5 

Salvo E, Garasa S, Dotor J, Morales X, Peláez R, Altevogt P and Rouzaut A: Combined targeting of TGF-β1 and integrin β3 impairs lymph node metastasis in a mouse model of non-small-cell lung cancer. Mol Cancer. 13:1122014. View Article : Google Scholar

6 

Li N, Zhang JP, Guo S, Min J, Liu LL, Su HC, Feng YM and Zhang HL: Down-regulation of β3-integrin inhibits bone metastasis of small cell lung cancer. Mol Biol Rep. 39:3029–3035. 2012. View Article : Google Scholar

7 

Parvani JG, Gujrati MD, Mack MA, Schiemann WP and Lu ZR: Silencing β3 integrin by targeted ECO/siRNA nanoparticles inhibits EMT and metastasis of triple-negative breast cancer. Cancer Res. 75:2316–2325. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Huveneers S and Danen EH: Adhesion signaling - crosstalk between integrins, Src and Rho. J Cell Sci. 122:1059–1069. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Raymond K, Faraldo MM, Deugnier MA and Glukhova MA: Integrins in mammary development. Semin Cell Dev Biol. 23:599–605. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Harquail J, Benzina S and Robichaud GA: MicroRNAs and breast cancer malignancy: an overview of miRNA-regulated cancer processes leading to metastasis. Cancer biomarkers: section. Dis Markers. 11:269–280. 2012.

11 

de Krijger I, Mekenkamp LJ, Punt CJ and Nagtegaal ID: MicroRNAs in colorectal cancer metastasis. J Pathol. 224:438–447. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Shi M and Guo N: MicroRNA expression and its implications for the diagnosis and therapeutic strategies of breast cancer. Cancer Treat Rev. 35:328–334. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Zhang N, Wang X, Huo Q, Sun M, Cai C, Liu Z, Hu G and Yang Q: MicroRNA-30a suppresses breast tumor growth and metastasis by targeting metadherin. Oncogene. 33:3119–3128. 2014. View Article : Google Scholar

14 

Kao CJ, Martiniez A, Shi XB, Yang J, Evans CP, Dobi A, deVere White RW and Kung HJ: miR-30 as a tumor suppressor connects EGF/Src signal to ERG and EMT. Oncogene. 33:2495–2503. 2014. View Article : Google Scholar

15 

Baraniskin A, Birkenkamp-Demtroder K, Maghnouj A, Zöllner H, Munding J, Klein-Scory S, Reinacher-Schick A, Schwarte-Waldhoff I, Schmiegel W and Hahn SA: MiR-30a-5p suppresses tumor growth in colon carcinoma by targeting DTL. Carcinogenesis. 33:732–739. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Hu G, Chong RA, Yang Q, Wei Y, Blanco MA, Li F, Reiss M, Au JL, Haffty BG and Kang Y: MTDH activation by 8q22 genomic gain promotes chemoresistance and metastasis of poor-prognosis breast cancer. Cancer Cell. 15:9–20. 2009. View Article : Google Scholar :

17 

Heckman KL and Pease LR: Gene splicing and mutagenesis by PCR-driven overlap extension. Nat Protoc. 2:924–932. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Knowles LM, Gurski LA, Engel C, Gnarra JR, Maranchie JK and Pilch J: Integrin αvβ3 and fibronectin upregulate Slug in cancer cells to promote clot invasion and metastasis. Cancer Res. 73:6175–6184. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Hynes RO: Integrins: Bidirectional, allosteric signaling machines. Cell. 110:673–687. 2002. View Article : Google Scholar : PubMed/NCBI

20 

Hynes RO: Targeted mutations in cell adhesion genes: What have we learned from them? Dev Biol. 180:402–412. 1996. View Article : Google Scholar : PubMed/NCBI

21 

Morgan MR, Humphries MJ and Bass MD: Synergistic control of cell adhesion by integrins and syndecans. Nat Rev Mol Cell Biol. 8:957–969. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Gehler S, Ponik SM, Riching KM and Keely PJ: Bi-directional signaling: Extracellular matrix and integrin regulation of breast tumor progression. Crit Rev Eukaryot Gene Expr. 23:139–157. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Truong HH, Xiong J, Ghotra VP, Nirmala E, Haazen L, Le Dévédec SE, Balcioğlu HE, He S, Snaar-Jagalska BE, Vreugdenhil E, et al: β1 integrin inhibition elicits a prometastatic switch through the TGFβ-miR-200-ZEB network in E-cadherin-positive triple-negative breast cancer. Sci Signal. 7:ra152014. View Article : Google Scholar

24 

Sumimoto S, Muramatsu R, Fujii S and Yamashita T: Vascular endothelial cells promote cortical neurite outgrowth via an integrin β3-dependent mechanism. Biochem Biophys Res Commun. 450:593–597. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Nasulewicz-Goldeman A, Uszczyńska B, Szczaurska-Nowak K and Wietrzyk J: siRNA-mediated silencing of integrin β3 expression inhibits the metastatic potential of B16 melanoma cells. Oncol Rep. 28:1567–1573. 2012.PubMed/NCBI

26 

Li A, Guo Q, Kim C, Hu W and Ye F: Integrin alphaII b tail distal of GFFKR participates in inside-out alphaII b beta3 activation. J Thromb Haemost. 12:1145–1155. 2014. View Article : Google Scholar : PubMed/NCBI

27 

Beauvais DM and Rapraeger AC: Syndecan-1-mediated cell spreading requires signaling by alphavbeta3 integrins in human breast carcinoma cells. Exp Cell Res. 286:219–232. 2003. View Article : Google Scholar : PubMed/NCBI

28 

Kudo-Saito C, Shirako H, Takeuchi T and Kawakami Y: Cancer metastasis is accelerated through immunosuppression during Snail-induced EMT of cancer cells. Cancer Cell. 15:195–206. 2009. View Article : Google Scholar : PubMed/NCBI

29 

Spaderna S, Schmalhofer O, Hlubek F, Berx G, Eger A, Merkel S, Jung A, Kirchner T and Brabletz T: A transient, EMT-linked loss of basement membranes indicates metastasis and poor survival in colorectal cancer. Gastroenterology. 131:830–840. 2006. View Article : Google Scholar : PubMed/NCBI

30 

Augoff K, Das M, Bialkowska K, McCue B, Plow EF and Sossey-Alaoui K: miR-31 is a broad regulator of β1-integrin expression and function in cancer cells. Mol Cancer Res. 9:1500–1508. 2011. View Article : Google Scholar : PubMed/NCBI

31 

Frijns E, Sachs N, Kreft M, Wilhelmsen K and Sonnenberg A: EGF-induced MAPK signaling inhibits hemidesmosome formation through phosphorylation of the integrin {beta}4. J Biol Chem. 285:37650–37662. 2010. View Article : Google Scholar : PubMed/NCBI

32 

Wei Y, Tang CH, Kim Y, Robillard L, Zhang F, Kugler MC and Chapman HA: Urokinase receptors are required for alpha 5 beta 1 integrin-mediated signaling in tumor cells. J Biol Chem. 282:3929–3939. 2007. View Article : Google Scholar

33 

Sato T and Miwa A: Ets-1 and integrin beta3 for lung metastasis from colorectal cancer. APMIS. 110:347–353. 2002. View Article : Google Scholar : PubMed/NCBI

34 

Rothhammer T, Hahne JC, Florin A, Poser I, Soncin F, Wernert N and Bosserhoff AK: The Ets-1 transcription factor is involved in the development and invasion of malignant melanoma. Cell Mol Life Sci. 61:118–128. 2004. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li W, Liu C, Zhao C, Zhai L and Lv S: Downregulation of β3 integrin by miR-30a-5p modulates cell adhesion and invasion by interrupting Erk/Ets‑1 network in triple-negative breast cancer Corrigendum in /10.3892/ijo.2021.5169. Int J Oncol 48: 1155-1164, 2016.
APA
Li, W., Liu, C., Zhao, C., Zhai, L., & Lv, S. (2016). Downregulation of β3 integrin by miR-30a-5p modulates cell adhesion and invasion by interrupting Erk/Ets‑1 network in triple-negative breast cancer Corrigendum in /10.3892/ijo.2021.5169. International Journal of Oncology, 48, 1155-1164. https://doi.org/10.3892/ijo.2016.3319
MLA
Li, W., Liu, C., Zhao, C., Zhai, L., Lv, S."Downregulation of β3 integrin by miR-30a-5p modulates cell adhesion and invasion by interrupting Erk/Ets‑1 network in triple-negative breast cancer Corrigendum in /10.3892/ijo.2021.5169". International Journal of Oncology 48.3 (2016): 1155-1164.
Chicago
Li, W., Liu, C., Zhao, C., Zhai, L., Lv, S."Downregulation of β3 integrin by miR-30a-5p modulates cell adhesion and invasion by interrupting Erk/Ets‑1 network in triple-negative breast cancer Corrigendum in /10.3892/ijo.2021.5169". International Journal of Oncology 48, no. 3 (2016): 1155-1164. https://doi.org/10.3892/ijo.2016.3319
Copy and paste a formatted citation
x
Spandidos Publications style
Li W, Liu C, Zhao C, Zhai L and Lv S: Downregulation of β3 integrin by miR-30a-5p modulates cell adhesion and invasion by interrupting Erk/Ets‑1 network in triple-negative breast cancer Corrigendum in /10.3892/ijo.2021.5169. Int J Oncol 48: 1155-1164, 2016.
APA
Li, W., Liu, C., Zhao, C., Zhai, L., & Lv, S. (2016). Downregulation of β3 integrin by miR-30a-5p modulates cell adhesion and invasion by interrupting Erk/Ets‑1 network in triple-negative breast cancer Corrigendum in /10.3892/ijo.2021.5169. International Journal of Oncology, 48, 1155-1164. https://doi.org/10.3892/ijo.2016.3319
MLA
Li, W., Liu, C., Zhao, C., Zhai, L., Lv, S."Downregulation of β3 integrin by miR-30a-5p modulates cell adhesion and invasion by interrupting Erk/Ets‑1 network in triple-negative breast cancer Corrigendum in /10.3892/ijo.2021.5169". International Journal of Oncology 48.3 (2016): 1155-1164.
Chicago
Li, W., Liu, C., Zhao, C., Zhai, L., Lv, S."Downregulation of β3 integrin by miR-30a-5p modulates cell adhesion and invasion by interrupting Erk/Ets‑1 network in triple-negative breast cancer Corrigendum in /10.3892/ijo.2021.5169". International Journal of Oncology 48, no. 3 (2016): 1155-1164. https://doi.org/10.3892/ijo.2016.3319
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