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Insulin-like growth factor 2 mRNA-binding protein-3 as a marker for distinguishing between cutaneous squamous cell carcinoma and keratoacanthoma

  • Authors:
    • Akiko Kanzaki
    • Mitsuhiro Kudo
    • Shin-Ichi Ansai
    • Wei-Xia Peng
    • Kousuke Ishino
    • Tetsushi Yamamoto
    • Ryuichi Wada
    • Takenori Fujii
    • Kiyoshi Teduka
    • Kiyoko Kawahara
    • Yoko Kawamoto
    • Taeko Kitamura
    • Seiji Kawana
    • Hidehisa Saeki
    • Zenya Naito
  • View Affiliations / Copyright

    Affiliations: Department of Integrated Diagnostic Pathology, Nippon Medical School, Tokyo, Japan, Department of Dermatology, Nippon Medical School Musashikosugi Hospital, Tokyo, Japan, Faculty of Pharmacy, Kinki University, Osaka, Japan, Department of Dermatology, Nippon Medical School, Tokyo, Japan
    Copyright: © Kanzaki et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1007-1015
    |
    Published online on: January 5, 2016
       https://doi.org/10.3892/ijo.2016.3323
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Abstract

In the histopathological diagnosis of cutaneous tumors, the differential diagnosis of squamous cell carcinoma (SCC) with crateriform architecture and keratoacanthoma (KA) is often difficult so an accurate understanding of the biological features and the identification of reliable markers of SCC and KA are crucial issues. Insulin-like growth factor 2 mRNA-binding protein-3 (IGF2BP3, also known as IMP3) is thought of as a bona fide oncofetal protein, which is overexpressed and is involved in cell proliferation, migration, and invasion in several kinds of tumors. However, the role of IMP3 in cutaneous SCC and KA has not been well studied. Therefore, we focused on studying the biological functions of IMP3 in SCC and KA. In human skin SCC cell lines, HSC-1 and HSC-5, and the human keratinocyte cell line, HaCaT, IMP3 mRNA levels were significantly higher than that of normal human skin. The knockdown of IMP3 expression reduced the proliferation of HSC-1, and significantly reduced invasion by HSC-1 and HSC-5. In contrast, the knockdown of IMP3 did not significantly affect invasion by HaCaT cells. In immunohistochemical studies of SCC and KA tissues, the Ki-67 labeling index (LI) of the suprabasal cell layer was significantly higher in SCC, compared with KA tissues and the tumor-free margin (TFM) adjacent to SCC and KA. Most SCC tissues stained strongly positive for IMP3, but KA tissues and TFM were mostly negative for IMP3. The Ki-67 LI of the IMP3-positive group was significantly higher than that of the IMP3-negative group in the suprabasal cell layer of SCC. These results suggest that IMP3 plays an important role in proliferation and, more significantly, in the invasion of SCC, and may be a suitable marker for the histopathological diagnosis of SCC with a crateriform architecture and KA. Furthermore, IMP3 may potentially be a new therapeutic target for SCC.
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Copy and paste a formatted citation
Spandidos Publications style
Kanzaki A, Kudo M, Ansai S, Peng W, Ishino K, Yamamoto T, Wada R, Fujii T, Teduka K, Kawahara K, Kawahara K, et al: Insulin-like growth factor 2 mRNA-binding protein-3 as a marker for distinguishing between cutaneous squamous cell carcinoma and keratoacanthoma. Int J Oncol 48: 1007-1015, 2016.
APA
Kanzaki, A., Kudo, M., Ansai, S., Peng, W., Ishino, K., Yamamoto, T. ... Naito, Z. (2016). Insulin-like growth factor 2 mRNA-binding protein-3 as a marker for distinguishing between cutaneous squamous cell carcinoma and keratoacanthoma. International Journal of Oncology, 48, 1007-1015. https://doi.org/10.3892/ijo.2016.3323
MLA
Kanzaki, A., Kudo, M., Ansai, S., Peng, W., Ishino, K., Yamamoto, T., Wada, R., Fujii, T., Teduka, K., Kawahara, K., Kawamoto, Y., Kitamura, T., Kawana, S., Saeki, H., Naito, Z."Insulin-like growth factor 2 mRNA-binding protein-3 as a marker for distinguishing between cutaneous squamous cell carcinoma and keratoacanthoma". International Journal of Oncology 48.3 (2016): 1007-1015.
Chicago
Kanzaki, A., Kudo, M., Ansai, S., Peng, W., Ishino, K., Yamamoto, T., Wada, R., Fujii, T., Teduka, K., Kawahara, K., Kawamoto, Y., Kitamura, T., Kawana, S., Saeki, H., Naito, Z."Insulin-like growth factor 2 mRNA-binding protein-3 as a marker for distinguishing between cutaneous squamous cell carcinoma and keratoacanthoma". International Journal of Oncology 48, no. 3 (2016): 1007-1015. https://doi.org/10.3892/ijo.2016.3323
Copy and paste a formatted citation
x
Spandidos Publications style
Kanzaki A, Kudo M, Ansai S, Peng W, Ishino K, Yamamoto T, Wada R, Fujii T, Teduka K, Kawahara K, Kawahara K, et al: Insulin-like growth factor 2 mRNA-binding protein-3 as a marker for distinguishing between cutaneous squamous cell carcinoma and keratoacanthoma. Int J Oncol 48: 1007-1015, 2016.
APA
Kanzaki, A., Kudo, M., Ansai, S., Peng, W., Ishino, K., Yamamoto, T. ... Naito, Z. (2016). Insulin-like growth factor 2 mRNA-binding protein-3 as a marker for distinguishing between cutaneous squamous cell carcinoma and keratoacanthoma. International Journal of Oncology, 48, 1007-1015. https://doi.org/10.3892/ijo.2016.3323
MLA
Kanzaki, A., Kudo, M., Ansai, S., Peng, W., Ishino, K., Yamamoto, T., Wada, R., Fujii, T., Teduka, K., Kawahara, K., Kawamoto, Y., Kitamura, T., Kawana, S., Saeki, H., Naito, Z."Insulin-like growth factor 2 mRNA-binding protein-3 as a marker for distinguishing between cutaneous squamous cell carcinoma and keratoacanthoma". International Journal of Oncology 48.3 (2016): 1007-1015.
Chicago
Kanzaki, A., Kudo, M., Ansai, S., Peng, W., Ishino, K., Yamamoto, T., Wada, R., Fujii, T., Teduka, K., Kawahara, K., Kawamoto, Y., Kitamura, T., Kawana, S., Saeki, H., Naito, Z."Insulin-like growth factor 2 mRNA-binding protein-3 as a marker for distinguishing between cutaneous squamous cell carcinoma and keratoacanthoma". International Journal of Oncology 48, no. 3 (2016): 1007-1015. https://doi.org/10.3892/ijo.2016.3323
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