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Article

Involvement of DDX6 gene in radio- and chemoresistance in glioblastoma

  • Authors:
    • Yu Jin Cho
    • Wonyoung Kang
    • Sung Heon Kim
    • Jason K. Sa
    • Nayoung Kim
    • Patrick J. Paddison
    • Misuk Kim
    • Kyeung Min Joo
    • Young-Il Hwang
    • Do-Hyun Nam
  • View Affiliations / Copyright

    Affiliations: Department of Anatomy, Seoul National University College of Medicine, Seoul, Republic of Korea, Samsung Biomedical Research Institute, Samsung Medical Center, Seoul, Republic of Korea, Department of Anatomy and Cell Biology, Sungkyunkwan University of Medicine, Suwon, Seoul, Republic of Korea, Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, Seoul, Republic of Korea, Department of Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA, Institute for Refractory Cancer Research, Samsung Medical Center, Seoul, Republic of Korea, Department of Neurosurgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea
  • Pages: 1053-1062
    |
    Published online on: January 11, 2016
       https://doi.org/10.3892/ijo.2016.3328
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Abstract

CCRT (concomitant chemotherapy and radiation therapy) is often used for glioblastoma multiforme (GBM) treatment after surgical therapy, however, patients treated with CCRT undergo poor prognosis due to development of treatment resistant recurrence. Many studies have been performed to overcome these problems and to discover genes influencing treatment resistance. To discover potential genes inducing CCRT resistance in GBM, we used whole genome screening by infecting shRNA pool in patient-derived cell. The cells infected ~8,000 shRNAs were implanted in mouse brain and treated RT/TMZ as in CCRT treated patients. We found DDX6 as the candidate gene for treatment resistance after screening and establishing DDX6 knock down cells for functional validation. Using these cells, we confirmed tumor associated ability of DDX6 in vitro and in vivo. Although proliferation improvement was not found, decreased DDX6 influenced upregulated clonogenic ability and resistant response against radiation treatment in vivo and in vitro. Taken together, we suggest that DDX6 discovered by using whole genome screening was responsible for radio- and chemoresistance in GBM.
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Copy and paste a formatted citation
Spandidos Publications style
Cho YJ, Kang W, Kim SH, Sa JK, Kim N, Paddison PJ, Kim M, Joo KM, Hwang Y, Nam D, Nam D, et al: Involvement of DDX6 gene in radio- and chemoresistance in glioblastoma . Int J Oncol 48: 1053-1062, 2016.
APA
Cho, Y.J., Kang, W., Kim, S.H., Sa, J.K., Kim, N., Paddison, P.J. ... Nam, D. (2016). Involvement of DDX6 gene in radio- and chemoresistance in glioblastoma . International Journal of Oncology, 48, 1053-1062. https://doi.org/10.3892/ijo.2016.3328
MLA
Cho, Y. J., Kang, W., Kim, S. H., Sa, J. K., Kim, N., Paddison, P. J., Kim, M., Joo, K. M., Hwang, Y., Nam, D."Involvement of DDX6 gene in radio- and chemoresistance in glioblastoma ". International Journal of Oncology 48.3 (2016): 1053-1062.
Chicago
Cho, Y. J., Kang, W., Kim, S. H., Sa, J. K., Kim, N., Paddison, P. J., Kim, M., Joo, K. M., Hwang, Y., Nam, D."Involvement of DDX6 gene in radio- and chemoresistance in glioblastoma ". International Journal of Oncology 48, no. 3 (2016): 1053-1062. https://doi.org/10.3892/ijo.2016.3328
Copy and paste a formatted citation
x
Spandidos Publications style
Cho YJ, Kang W, Kim SH, Sa JK, Kim N, Paddison PJ, Kim M, Joo KM, Hwang Y, Nam D, Nam D, et al: Involvement of DDX6 gene in radio- and chemoresistance in glioblastoma . Int J Oncol 48: 1053-1062, 2016.
APA
Cho, Y.J., Kang, W., Kim, S.H., Sa, J.K., Kim, N., Paddison, P.J. ... Nam, D. (2016). Involvement of DDX6 gene in radio- and chemoresistance in glioblastoma . International Journal of Oncology, 48, 1053-1062. https://doi.org/10.3892/ijo.2016.3328
MLA
Cho, Y. J., Kang, W., Kim, S. H., Sa, J. K., Kim, N., Paddison, P. J., Kim, M., Joo, K. M., Hwang, Y., Nam, D."Involvement of DDX6 gene in radio- and chemoresistance in glioblastoma ". International Journal of Oncology 48.3 (2016): 1053-1062.
Chicago
Cho, Y. J., Kang, W., Kim, S. H., Sa, J. K., Kim, N., Paddison, P. J., Kim, M., Joo, K. M., Hwang, Y., Nam, D."Involvement of DDX6 gene in radio- and chemoresistance in glioblastoma ". International Journal of Oncology 48, no. 3 (2016): 1053-1062. https://doi.org/10.3892/ijo.2016.3328
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