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Article

Twist mediates an aggressive phenotype in human colorectal cancer cells

  • Authors:
    • Jun-Jian Deng
    • Wei Zhang
    • Xi-Ming Xu
    • Fan Zhang
    • Wei-Ping Tao
    • Jun-Jie Ye
    • Wei Ge
  • View Affiliations / Copyright

    Affiliations: Department of Cancer Center, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China, Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China, Department of Pharmacy, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
  • Pages: 1117-1124
    |
    Published online on: January 15, 2016
       https://doi.org/10.3892/ijo.2016.3342
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Abstract

Epithelial-mesenchymal transition (EMT) is a crucial process providing cancer cells with the ability to migrate and metastasize to distant sites. Recently, EMT was shown to be associated with the cancer stem cell (CSC) phenotype and chemoresistance. Twist is a transcription factor that regulates EMT in a various cancer cells, including colorectal cancer (CRC). Our study was done to determine the role of Twist in mediating aggressive phenotype in CRC. Human CRC cell lines were transduced with a retroviral Twist construct or vector control. Migration and invasion abilities were determined in vitro using modified Boyden chamber assays. Mammosphere formation assay was performed to detect CSC characteristics. EMT and CSC markers were detected using western blotting and RT-PCR. Chemosensitivity to oxaliplatin of the transfected cells were determined by the MTT assay. Human CRC specimens were stained for Twist and P-gp expression. Twist overexpression triggered EMT and a CSC-like phenotype in human CRC cells and enhanced cell migration, invasion and mammosphere formation abilities. In addition, Twist-overexpressing CRC cells were more chemo­resistant to oxaliplatin than control cells. Furthermore, Twist over­expression increased P-gp expression in CRC cells, which is a transmembrane glycoprotein conferred multidrug-resistance phenotype to various cancer cells. Importantly, Twist and P-gp were expressed correlatively in human CRC specimens. Thus, Twist is a potential therapeutic target in metastatic CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Deng J, Zhang W, Xu X, Zhang F, Tao W, Ye J and Ge W: Twist mediates an aggressive phenotype in human colorectal cancer cells. Int J Oncol 48: 1117-1124, 2016.
APA
Deng, J., Zhang, W., Xu, X., Zhang, F., Tao, W., Ye, J., & Ge, W. (2016). Twist mediates an aggressive phenotype in human colorectal cancer cells. International Journal of Oncology, 48, 1117-1124. https://doi.org/10.3892/ijo.2016.3342
MLA
Deng, J., Zhang, W., Xu, X., Zhang, F., Tao, W., Ye, J., Ge, W."Twist mediates an aggressive phenotype in human colorectal cancer cells". International Journal of Oncology 48.3 (2016): 1117-1124.
Chicago
Deng, J., Zhang, W., Xu, X., Zhang, F., Tao, W., Ye, J., Ge, W."Twist mediates an aggressive phenotype in human colorectal cancer cells". International Journal of Oncology 48, no. 3 (2016): 1117-1124. https://doi.org/10.3892/ijo.2016.3342
Copy and paste a formatted citation
x
Spandidos Publications style
Deng J, Zhang W, Xu X, Zhang F, Tao W, Ye J and Ge W: Twist mediates an aggressive phenotype in human colorectal cancer cells. Int J Oncol 48: 1117-1124, 2016.
APA
Deng, J., Zhang, W., Xu, X., Zhang, F., Tao, W., Ye, J., & Ge, W. (2016). Twist mediates an aggressive phenotype in human colorectal cancer cells. International Journal of Oncology, 48, 1117-1124. https://doi.org/10.3892/ijo.2016.3342
MLA
Deng, J., Zhang, W., Xu, X., Zhang, F., Tao, W., Ye, J., Ge, W."Twist mediates an aggressive phenotype in human colorectal cancer cells". International Journal of Oncology 48.3 (2016): 1117-1124.
Chicago
Deng, J., Zhang, W., Xu, X., Zhang, F., Tao, W., Ye, J., Ge, W."Twist mediates an aggressive phenotype in human colorectal cancer cells". International Journal of Oncology 48, no. 3 (2016): 1117-1124. https://doi.org/10.3892/ijo.2016.3342
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