Open Access

Direct regulation of LAMP1 by tumor-suppressive microRNA-320a in prostate cancer

  • Authors:
    • Atsushi Okato
    • Yusuke Goto
    • Akira Kurozumi
    • Mayuko Kato
    • Satoko Kojima
    • Ryosuke Matsushita
    • Masaya Yonemori
    • Kazutaka Miyamoto
    • Tomohiko Ichikawa
    • Naohiko Seki
  • View Affiliations

  • Published online on: May 16, 2016     https://doi.org/10.3892/ijo.2016.3522
  • Pages: 111-122
  • Copyright: © Okato et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Advanced prostate cancer (PCa) metastasizes to bone and lymph nodes, and currently available treatments cannot prevent the progression and metastasis of the disease. Therefore, an improved understanding of the molecular mechanisms of the progression and metastasis of advanced PCa using current genomic approaches is needed. Our miRNA expression signature in castration-resistant prostate cancer (CRPC) revealed that microRNA-320a (miR‑320a) was significantly reduced in cancer tissues, suggesting that miR‑320a may be a promising anticancer miRNA. The aim of this study was to investigate the functional roles of miR‑320a in naïve PCa and CRPC cells and to identify miR‑320a-regulated genes involved in PCa metastasis. The expression levels of miR‑320a were significantly reduced in naïve PCa, CRPC specimens, and PCa cell lines. Restoration of mature miR‑320a in PCa cell lines showed that miR‑320a significantly inhibited cancer cell migration and invasion. Moreover, we found that lysosomal-associated membrane protein 1 (LAMP1) was a direct target of miR‑320a in PCa cells. Silencing of LAMP1 using siRNA significantly inhibited cell proliferation, migration, and invasion in PCa cells. Overexpression of LAMP1 was observed in PCa and CRPC clinical specimens. Moreover, downstream pathways were identified using si-LAMP1-transfected cells. The discovery of tumor-suppressive miR‑320a-mediated pathways may provide important insights into the potential mechanisms of PCa metastasis.
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July-2016
Volume 49 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Okato A, Goto Y, Kurozumi A, Kato M, Kojima S, Matsushita R, Yonemori M, Miyamoto K, Ichikawa T, Seki N, Seki N, et al: Direct regulation of LAMP1 by tumor-suppressive microRNA-320a in prostate cancer. Int J Oncol 49: 111-122, 2016
APA
Okato, A., Goto, Y., Kurozumi, A., Kato, M., Kojima, S., Matsushita, R. ... Seki, N. (2016). Direct regulation of LAMP1 by tumor-suppressive microRNA-320a in prostate cancer. International Journal of Oncology, 49, 111-122. https://doi.org/10.3892/ijo.2016.3522
MLA
Okato, A., Goto, Y., Kurozumi, A., Kato, M., Kojima, S., Matsushita, R., Yonemori, M., Miyamoto, K., Ichikawa, T., Seki, N."Direct regulation of LAMP1 by tumor-suppressive microRNA-320a in prostate cancer". International Journal of Oncology 49.1 (2016): 111-122.
Chicago
Okato, A., Goto, Y., Kurozumi, A., Kato, M., Kojima, S., Matsushita, R., Yonemori, M., Miyamoto, K., Ichikawa, T., Seki, N."Direct regulation of LAMP1 by tumor-suppressive microRNA-320a in prostate cancer". International Journal of Oncology 49, no. 1 (2016): 111-122. https://doi.org/10.3892/ijo.2016.3522