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Differential effects of rapalogues, dual kinase inhibitors on human ovarian carcinoma cells in vitro

  • Authors:
    • Karly-Rai Rogers-Broadway
    • Dimple Chudasama
    • George Pados
    • Dimitris Tsolakidis
    • Anastasia Goumenou
    • Marcia Hall
    • Emmanouil Karteris
  • View Affiliations / Copyright

    Affiliations: Biosciences, College of Health and Life Sciences, Brunel University London, Uxbridge, UB8 3PH, UK, Medical School, Aristotle University, Thessaloniki, Greece
    Copyright: © Rogers-Broadway et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 133-143
    |
    Published online on: May 18, 2016
       https://doi.org/10.3892/ijo.2016.3531
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Abstract

Ovarian cancer is the second most common gynaecological malignancy and was diagnosed in over 7,000 women in 2011 in the UK. There are currently no reliable biomarkers available for use in a regular screening assay for ovarian cancer and due to characteristic late presentation (78% in stages III and IV) ovarian cancer has a low survival rate (35% after 10 years). The mTOR pathway is a central regulator of growth, proliferation, apoptosis and angiogenesis; providing balance between available resources such as amino acids and growth factors, and stresses such as hypoxia, to control cellular behaviour accordingly. Emerging data links mTOR with the aetiopathogenesis of ovarian cancer. We hypothesised that mTOR inhibitors could play a therapeutic role in ovarian cancer treatment. In this study we began by validating the expression of four main mTOR pathway components, mTOR, DEPTOR, rictor and raptor, at gene and protein level in in vitro models of endometrioid (MDAH‑2774) and clear cell (SKOV3) ovarian cancer using qPCR and ImageStream technology. Using a wound healing assay we show that inhibition of the mTOR pathway using rapamycin, rapalogues, resveratrol and NVP BEZ-235 induces a cytostatic and not cytotoxic response up to 18 h in these cell lines. We extended these findings up to 72 h with a proliferation assay and show that the effects of inhibition of the mTOR pathway are primarily mediated by the dephosphorylation of p70S6 kinase. We show that mTOR inhibition does not involve alteration of mTOR pathway components or induce caspase 9 cleavage. Preclinical studies including ovarian tissue of ovarian cancer patients, unaffected controls and patients with unrelated gynaecological conditions show that DEPTOR is reliably upregulated in ovarian cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Rogers-Broadway K, Chudasama D, Pados G, Tsolakidis D, Goumenou A, Hall M and Karteris E: Differential effects of rapalogues, dual kinase inhibitors on human ovarian carcinoma cells in vitro. Int J Oncol 49: 133-143, 2016.
APA
Rogers-Broadway, K., Chudasama, D., Pados, G., Tsolakidis, D., Goumenou, A., Hall, M., & Karteris, E. (2016). Differential effects of rapalogues, dual kinase inhibitors on human ovarian carcinoma cells in vitro. International Journal of Oncology, 49, 133-143. https://doi.org/10.3892/ijo.2016.3531
MLA
Rogers-Broadway, K., Chudasama, D., Pados, G., Tsolakidis, D., Goumenou, A., Hall, M., Karteris, E."Differential effects of rapalogues, dual kinase inhibitors on human ovarian carcinoma cells in vitro". International Journal of Oncology 49.1 (2016): 133-143.
Chicago
Rogers-Broadway, K., Chudasama, D., Pados, G., Tsolakidis, D., Goumenou, A., Hall, M., Karteris, E."Differential effects of rapalogues, dual kinase inhibitors on human ovarian carcinoma cells in vitro". International Journal of Oncology 49, no. 1 (2016): 133-143. https://doi.org/10.3892/ijo.2016.3531
Copy and paste a formatted citation
x
Spandidos Publications style
Rogers-Broadway K, Chudasama D, Pados G, Tsolakidis D, Goumenou A, Hall M and Karteris E: Differential effects of rapalogues, dual kinase inhibitors on human ovarian carcinoma cells in vitro. Int J Oncol 49: 133-143, 2016.
APA
Rogers-Broadway, K., Chudasama, D., Pados, G., Tsolakidis, D., Goumenou, A., Hall, M., & Karteris, E. (2016). Differential effects of rapalogues, dual kinase inhibitors on human ovarian carcinoma cells in vitro. International Journal of Oncology, 49, 133-143. https://doi.org/10.3892/ijo.2016.3531
MLA
Rogers-Broadway, K., Chudasama, D., Pados, G., Tsolakidis, D., Goumenou, A., Hall, M., Karteris, E."Differential effects of rapalogues, dual kinase inhibitors on human ovarian carcinoma cells in vitro". International Journal of Oncology 49.1 (2016): 133-143.
Chicago
Rogers-Broadway, K., Chudasama, D., Pados, G., Tsolakidis, D., Goumenou, A., Hall, M., Karteris, E."Differential effects of rapalogues, dual kinase inhibitors on human ovarian carcinoma cells in vitro". International Journal of Oncology 49, no. 1 (2016): 133-143. https://doi.org/10.3892/ijo.2016.3531
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