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Article

Transcriptional regulation of E-cadherin by small activating RNA: A new double-stranded RNA

  • Authors:
    • Zhiming Wu
    • Yan Li
    • Zhiyong Li
    • Zhuowei Liu
    • Zike Qin
    • Xiangdong Li
    • Yunlin Ye
    • Lei Bu
    • Bin Lin
    • Zhanyu Wang
    • Guojin Jia
    • Gang Chen
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, Guangdong, P.R. China, Department of Urology, Jinshan Hospital, Fudan University, Shanghai, P.R. China, Langone Medical Center School of Medicine, NYU, New York, NY, USA
  • Pages: 1620-1628
    |
    Published online on: August 3, 2016
       https://doi.org/10.3892/ijo.2016.3643
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Abstract

Recent studies have reported that chemically synthesized small activating RNA (saRNA) targeting the promoter regions of a gene can activate its expression in different cell lines. This technique can be a powerful therapeutic method for diseases caused by complete inactivation or reduced expression of specific genes. E-cadherin is a typical tumor suppressor gene. Loss of E-cadherin mediates the transition from benign lesions to invasive, metastatic cancer. In this study, several 21-nt small double-stranded RNAs (dsRNAs) targeting the promoter regions of human E-cadherin were designed and synthesized and the features of their function were investigated to study the regulatory role of dsRNA on E-cadherin expression. A new saRNA (dsEcad‑661) that can enhance E-cadherin expression by targeting non-coding regulatory regions in gene promoters was identified. Using dsRNA with modified base quantity and cholesterol-conjugated dsRNA, we found the antisense strand may be the guide strand of saRNA in the upregulation of E-cadherin. These findings provide several important pieces of evidence that may improve understanding of the function of saRNA and may promote its development for clinical application.
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Copy and paste a formatted citation
Spandidos Publications style
Wu Z, Li Y, Li Z, Liu Z, Qin Z, Li X, Ye Y, Bu L, Lin B, Wang Z, Wang Z, et al: Transcriptional regulation of E-cadherin by small activating RNA: A new double-stranded RNA. Int J Oncol 49: 1620-1628, 2016.
APA
Wu, Z., Li, Y., Li, Z., Liu, Z., Qin, Z., Li, X. ... Chen, G. (2016). Transcriptional regulation of E-cadherin by small activating RNA: A new double-stranded RNA. International Journal of Oncology, 49, 1620-1628. https://doi.org/10.3892/ijo.2016.3643
MLA
Wu, Z., Li, Y., Li, Z., Liu, Z., Qin, Z., Li, X., Ye, Y., Bu, L., Lin, B., Wang, Z., Jia, G., Chen, G."Transcriptional regulation of E-cadherin by small activating RNA: A new double-stranded RNA". International Journal of Oncology 49.4 (2016): 1620-1628.
Chicago
Wu, Z., Li, Y., Li, Z., Liu, Z., Qin, Z., Li, X., Ye, Y., Bu, L., Lin, B., Wang, Z., Jia, G., Chen, G."Transcriptional regulation of E-cadherin by small activating RNA: A new double-stranded RNA". International Journal of Oncology 49, no. 4 (2016): 1620-1628. https://doi.org/10.3892/ijo.2016.3643
Copy and paste a formatted citation
x
Spandidos Publications style
Wu Z, Li Y, Li Z, Liu Z, Qin Z, Li X, Ye Y, Bu L, Lin B, Wang Z, Wang Z, et al: Transcriptional regulation of E-cadherin by small activating RNA: A new double-stranded RNA. Int J Oncol 49: 1620-1628, 2016.
APA
Wu, Z., Li, Y., Li, Z., Liu, Z., Qin, Z., Li, X. ... Chen, G. (2016). Transcriptional regulation of E-cadherin by small activating RNA: A new double-stranded RNA. International Journal of Oncology, 49, 1620-1628. https://doi.org/10.3892/ijo.2016.3643
MLA
Wu, Z., Li, Y., Li, Z., Liu, Z., Qin, Z., Li, X., Ye, Y., Bu, L., Lin, B., Wang, Z., Jia, G., Chen, G."Transcriptional regulation of E-cadherin by small activating RNA: A new double-stranded RNA". International Journal of Oncology 49.4 (2016): 1620-1628.
Chicago
Wu, Z., Li, Y., Li, Z., Liu, Z., Qin, Z., Li, X., Ye, Y., Bu, L., Lin, B., Wang, Z., Jia, G., Chen, G."Transcriptional regulation of E-cadherin by small activating RNA: A new double-stranded RNA". International Journal of Oncology 49, no. 4 (2016): 1620-1628. https://doi.org/10.3892/ijo.2016.3643
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