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International Journal of Oncology
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Article

Tricin, 4',5,7-trihydroxy-3',5'-dimethoxyflavone, exhibits potent antiangiogenic activity in vitro

  • Authors:
    • Jang Mi Han
    • Ho Jeong Kwon
    • Hye Jin Jung
  • View Affiliations / Copyright

    Affiliations: Department of BT-Convergent Pharmaceutical Engineering, Sun Moon University, Chungnam 336-708, Republic of Korea, Department of Biotechnology, Yonsei University, Seodaemun-gu, Seoul 120-749, Republic of Korea
  • Pages: 1497-1504
    |
    Published online on: August 3, 2016
       https://doi.org/10.3892/ijo.2016.3645
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Abstract

Tumor growth and metastasis depend on angiogenesis triggered by chemical signals, such as vascular endothelial growth factor (VEGF), released from tumor cells. Therefore, the specific perturbation of angiogenesis has been considered a powerful strategy for the treatment of cancer. Herein, we report that tricin, 4',5,7-trihydroxy-3',5'-dimethoxyflavone, exhibits potent antiangiogenic activity in vitro. Tricin effectively suppressed the proliferation as well as VEGF-induced invasion and tube formation of human umbilical vein endothelial cells (HUVECs) at subtoxic doses. Furthermore, tricin significantly inhibited the angiogenesis of the chorioallantoic membrane from growing chick embryos without showing cytotoxicity. We also found that tricin blocked tumor cell-induced angiogenesis. Notably, tricin downregulated not only the VEGFR2 signal transduction by reducing reactive oxygen species (ROS) generation in endothelial cells, but also the expression of VEGF by inhibiting hypoxia inducible factor-1α (HIF-1α) accumulation in tumor cells. Moreover, combined treatment with tricin and bevacizumab, an anti-VEGF drug, ameliorated the antiangiogenic effect of bevacizumab. Taken together, our findings demonstrate for the first time that tricin possesses promising antiangiogenic potential and thus may be applied to anticancer therapy by targeting tumor angiogenesis.
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1 

Bussolino F, Mantovani A and Persico G: Molecular mechanisms of blood vessel formation. Trends Biochem Sci. 22:251–256. 1997. View Article : Google Scholar : PubMed/NCBI

2 

Folkman J: Seminars in Medicine of the Beth Israel Hospital, Boston. Clinical applications of research on angiogenesis. N Engl J Med. 333:1757–1763. 1995. View Article : Google Scholar : PubMed/NCBI

3 

Carmeliet P: Angiogenesis in health and disease. Nat Med. 9:653–660. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Bergers G and Benjamin LE: Tumorigenesis and the angiogenic switch. Nat Rev Cancer. 3:401–410. 2003. View Article : Google Scholar : PubMed/NCBI

5 

André T, Chastre E, Kotelevets L, Vaillant JC, Louvet C, Balosso J, Le Gall E, Prévot S and Gespach C: Tumoral angiogenesis: Physiopathology, prognostic value and therapeutic perspectives. Rev Med Interne. 19:904–913. 1998.(In French). View Article : Google Scholar

6 

Ferrara N: VEGF and the quest for tumour angiogenesis factors. Nat Rev Cancer. 2:795–803. 2002. View Article : Google Scholar : PubMed/NCBI

7 

Hicklin DJ and Ellis LM: Role of the vascular endothelial growth factor pathway in tumor growth and angiogenesis. J Clin Oncol. 23:1011–1027. 2005. View Article : Google Scholar

8 

Holmes K, Roberts OL, Thomas AM and Cross MJ: Vascular endothelial growth factor receptor-2: Structure, function, intracellular signalling and therapeutic inhibition. Cell Signal. 19:2003–2012. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Ferrara N, Hillan KJ, Gerber HP and Novotny W: Discovery and development of bevacizumab, an anti-VEGF antibody for treating cancer. Nat Rev Drug Discov. 3:391–400. 2004. View Article : Google Scholar : PubMed/NCBI

10 

Ivy SP, Wick JY and Kaufman BM: An overview of small-molecule inhibitors of VEGFR signaling. Nat Rev Clin Oncol. 6:569–579. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Chen HX and Cleck JN: Adverse effects of anticancer agents that target the VEGF pathway. Nat Rev Clin Oncol. 6:465–477. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Verheul HM and Pinedo HM: Possible molecular mechanisms involved in the toxicity of angiogenesis inhibition. Nat Rev Cancer. 7:475–485. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Höckel M and Vaupel P: Tumor hypoxia: Definitions and current clinical, biologic, and molecular aspects. J Natl Cancer Inst. 93:266–276. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Forsythe JA, Jiang BH, Iyer NV, Agani F, Leung SW, Koos RD and Semenza GL: Activation of vascular endothelial growth factor gene transcription by hypoxia-inducible factor 1. Mol Cell Biol. 16:4604–4613. 1996. View Article : Google Scholar : PubMed/NCBI

15 

Semenza GL: HIF-1 and tumor progression: Pathophysiology and therapeutics. Trends Mol Med. 8(Suppl): S62–S67. 2002. View Article : Google Scholar : PubMed/NCBI

16 

Semenza GL: Targeting HIF-1 for cancer therapy. Nat Rev Cancer. 3:721–732. 2003. View Article : Google Scholar : PubMed/NCBI

17 

Harborne JB: Nature, distribution and function of plant flavonoids. Prog Clin Biol Res. 213:15–24. 1986.PubMed/NCBI

18 

Havsteen BH: The biochemistry and medical significance of the flavonoids. Pharmacol Ther. 96:67–202. 2002. View Article : Google Scholar : PubMed/NCBI

19 

Cai H, Hudson EA, Mann P, Verschoyle RD, Greaves P, Manson MM, Steward WP and Gescher AJ: Growth-inhibitory and cell cycle-arresting properties of the rice bran constituent tricin in human-derived breast cancer cells in vitro and in nude mice in vivo. Br J Cancer. 91:1364–1371. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Hudson EA, Dinh PA, Kokubun T, Simmonds MS and Gescher A: Characterization of potentially chemopreventive phenols in extracts of brown rice that inhibit the growth of human breast and colon cancer cells. Cancer Epidemiol Biomarkers Prev. 9:1163–1170. 2000.PubMed/NCBI

21 

Oyama T, Yasui Y, Sugie S, Koketsu M, Watanabe K and Tanaka T: Dietary tricin suppresses inflammation-related colon carcinogenesis in male Crj: CD-1 mice. Cancer Prev Res (Phila). 2:1031–1038. 2009. View Article : Google Scholar

22 

Ali MA, Choy H, Habib AA and Saha D: SNS-032 prevents tumor cell-induced angiogenesis by inhibiting vascular endothelial growth factor. Neoplasia. 9:370–381. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Ushio-Fukai M: VEGF signaling through NADPH oxidase-derived ROS. Antioxid Redox Signal. 9:731–739. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Jung HJ, Kim Y, Chang J, Kang SW, Kim JH and Kwon HJ: Mitochondrial UQCRB regulates VEGFR2 signaling in endothelial cells. J Mol Med (Berl). 91:1117–1128. 2013. View Article : Google Scholar

25 

Bergers G and Hanahan D: Modes of resistance to anti-angiogenic therapy. Nat Rev Cancer. 8:592–603. 2008. View Article : Google Scholar : PubMed/NCBI

26 

El-Kenawi AE and El-Remessy AB: Angiogenesis inhibitors in cancer therapy: Mechanistic perspective on classification and treatment rationales. Br J Pharmacol. 170:712–729. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Zhou J and Ibrahim RK: Tricin - a potential multifunctional nutraceutical. Phytochem Rev. 9:413–424. 2010. View Article : Google Scholar

28 

Shalini V, Pushpan CK, Sindhu G, Jayalekshmi A and Helen A: Tricin, flavonoid from Njavara reduces inflammatory responses in hPBMCs by modulating the p38MAPK and PI3K/Akt pathways and prevents inflammation associated endothelial dysfunction in HUVECs. Immunobiology. 221:137–144. 2016. View Article : Google Scholar

29 

Shalini V, Jayalekshmi A and Helen A: Mechanism of anti-inflammatory effect of tricin, a flavonoid isolated from Njavara rice bran in LPS induced hPBMCs and carrageenan induced rats. Mol Immunol. 66:229–239. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Cai H, Al-Fayez M, Tunstall RG, Platton S, Greaves P, Steward WP and Gescher AJ: The rice bran constituent tricin potently inhibits cyclooxygenase enzymes and interferes with intestinal carcinogenesis in ApcMin mice. Mol Cancer Ther. 4:1287–1292. 2005. View Article : Google Scholar : PubMed/NCBI

31 

Jeong YH, Chung SY, Han AR, Sung MK, Jang DS, Lee J, Kwon Y, Lee HJ and Seo EK: P-glycoprotein inhibitory activity of two phenolic compounds, (−)-syringaresinol and tricin from Sasa borealis. Chem Biodivers. 4:12–16. 2007. View Article : Google Scholar : PubMed/NCBI

32 

Cai H, Boocock DJ, Steward WP and Gescher AJ: Tissue distribution in mice and metabolism in murine and human liver of apigenin and tricin, flavones with putative cancer chemopreventive properties. Cancer Chemother Pharmacol. 60:257–266. 2007. View Article : Google Scholar

33 

Verschoyle RD, Greaves P, Cai H, Borkhardt A, Broggini M, D’Incalci M, Riccio E, Doppalapudi R, Kapetanovic IM, Steward WP, et al: Preliminary safety evaluation of the putative cancer chemopreventive agent tricin, a naturally occurring flavone. Cancer Chemother Pharmacol. 57:1–6. 2006. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Han JM, Kwon HJ and Jung HJ: Tricin, 4',5,7-trihydroxy-3',5'-dimethoxyflavone, exhibits potent antiangiogenic activity in vitro. Int J Oncol 49: 1497-1504, 2016.
APA
Han, J.M., Kwon, H.J., & Jung, H.J. (2016). Tricin, 4',5,7-trihydroxy-3',5'-dimethoxyflavone, exhibits potent antiangiogenic activity in vitro. International Journal of Oncology, 49, 1497-1504. https://doi.org/10.3892/ijo.2016.3645
MLA
Han, J. M., Kwon, H. J., Jung, H. J."Tricin, 4',5,7-trihydroxy-3',5'-dimethoxyflavone, exhibits potent antiangiogenic activity in vitro". International Journal of Oncology 49.4 (2016): 1497-1504.
Chicago
Han, J. M., Kwon, H. J., Jung, H. J."Tricin, 4',5,7-trihydroxy-3',5'-dimethoxyflavone, exhibits potent antiangiogenic activity in vitro". International Journal of Oncology 49, no. 4 (2016): 1497-1504. https://doi.org/10.3892/ijo.2016.3645
Copy and paste a formatted citation
x
Spandidos Publications style
Han JM, Kwon HJ and Jung HJ: Tricin, 4',5,7-trihydroxy-3',5'-dimethoxyflavone, exhibits potent antiangiogenic activity in vitro. Int J Oncol 49: 1497-1504, 2016.
APA
Han, J.M., Kwon, H.J., & Jung, H.J. (2016). Tricin, 4',5,7-trihydroxy-3',5'-dimethoxyflavone, exhibits potent antiangiogenic activity in vitro. International Journal of Oncology, 49, 1497-1504. https://doi.org/10.3892/ijo.2016.3645
MLA
Han, J. M., Kwon, H. J., Jung, H. J."Tricin, 4',5,7-trihydroxy-3',5'-dimethoxyflavone, exhibits potent antiangiogenic activity in vitro". International Journal of Oncology 49.4 (2016): 1497-1504.
Chicago
Han, J. M., Kwon, H. J., Jung, H. J."Tricin, 4',5,7-trihydroxy-3',5'-dimethoxyflavone, exhibits potent antiangiogenic activity in vitro". International Journal of Oncology 49, no. 4 (2016): 1497-1504. https://doi.org/10.3892/ijo.2016.3645
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