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International Journal of Oncology
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Article

Anti-invasion and anti-migration effects of miR-199a-3p in hepatocellular carcinoma are due in part to targeting CD151

  • Authors:
    • Ji Hye Kim
    • Mohamed Badawi
    • Jong-Kook Park
    • Jinmai Jiang
    • Xiaokui Mo
    • Lewis R. Roberts
    • Thomas D. Schmittgen
  • View Affiliations / Copyright

    Affiliations: College of Pharmacy, Ohio State University, Columbus, OH, USA, Department of Biostatistics, Ohio State University, Columbus, OH, USA, Divisions of Gastroenterology and Hepatology, Mayo Clinic College of Medicine, Rochester, MN, USA
  • Pages: 2037-2045
    |
    Published online on: September 2, 2016
       https://doi.org/10.3892/ijo.2016.3677
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Abstract

Several studies have reported reduced miR-199a-3p expression in hepatocellular carcinoma (HCC). In an effort to discover important target genes for miR-199a-3p that may be related to HCC development or progression, we identified the tetraspanin, transmembrane glycoprotein CD151. Luciferase reporter assays and western blotting identified CD151 as a bona fide miR-199a-3p target gene. While CD151 protein was increased in the mesenchymal but not the epithelial HCC cell lines, CD151 knockdown with siRNA did not reduce HCC cell proliferation in either group of cells. miR-199a-3p reduced in vitro invasion and migration of CD151-positive HCC cells. Examination of the mRNA and protein expression in pairs of primary HCC tumors and adjacent benign tissues showed that not only was CD151 mRNA and protein increased in the tumors but also that an inverse correlation exists between the miR-199a-3p and CD151 RNA expression. We report that CD151 is a target of miR-199a-3p and that increased CD151 protein resulting from reduced miR-199a-3p could contribute to the development of metastatic HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Kim JH, Badawi M, Park J, Jiang J, Mo X, Roberts LR and Schmittgen TD: Anti-invasion and anti-migration effects of miR-199a-3p in hepatocellular carcinoma are due in part to targeting CD151. Int J Oncol 49: 2037-2045, 2016.
APA
Kim, J.H., Badawi, M., Park, J., Jiang, J., Mo, X., Roberts, L.R., & Schmittgen, T.D. (2016). Anti-invasion and anti-migration effects of miR-199a-3p in hepatocellular carcinoma are due in part to targeting CD151. International Journal of Oncology, 49, 2037-2045. https://doi.org/10.3892/ijo.2016.3677
MLA
Kim, J. H., Badawi, M., Park, J., Jiang, J., Mo, X., Roberts, L. R., Schmittgen, T. D."Anti-invasion and anti-migration effects of miR-199a-3p in hepatocellular carcinoma are due in part to targeting CD151". International Journal of Oncology 49.5 (2016): 2037-2045.
Chicago
Kim, J. H., Badawi, M., Park, J., Jiang, J., Mo, X., Roberts, L. R., Schmittgen, T. D."Anti-invasion and anti-migration effects of miR-199a-3p in hepatocellular carcinoma are due in part to targeting CD151". International Journal of Oncology 49, no. 5 (2016): 2037-2045. https://doi.org/10.3892/ijo.2016.3677
Copy and paste a formatted citation
x
Spandidos Publications style
Kim JH, Badawi M, Park J, Jiang J, Mo X, Roberts LR and Schmittgen TD: Anti-invasion and anti-migration effects of miR-199a-3p in hepatocellular carcinoma are due in part to targeting CD151. Int J Oncol 49: 2037-2045, 2016.
APA
Kim, J.H., Badawi, M., Park, J., Jiang, J., Mo, X., Roberts, L.R., & Schmittgen, T.D. (2016). Anti-invasion and anti-migration effects of miR-199a-3p in hepatocellular carcinoma are due in part to targeting CD151. International Journal of Oncology, 49, 2037-2045. https://doi.org/10.3892/ijo.2016.3677
MLA
Kim, J. H., Badawi, M., Park, J., Jiang, J., Mo, X., Roberts, L. R., Schmittgen, T. D."Anti-invasion and anti-migration effects of miR-199a-3p in hepatocellular carcinoma are due in part to targeting CD151". International Journal of Oncology 49.5 (2016): 2037-2045.
Chicago
Kim, J. H., Badawi, M., Park, J., Jiang, J., Mo, X., Roberts, L. R., Schmittgen, T. D."Anti-invasion and anti-migration effects of miR-199a-3p in hepatocellular carcinoma are due in part to targeting CD151". International Journal of Oncology 49, no. 5 (2016): 2037-2045. https://doi.org/10.3892/ijo.2016.3677
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