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Distinct expression of C4.4A in colorectal cancer detected by different antibodies

HIROFUMI YAMAMOTO, RYOTA OSHIRO, MASAHISA OHTSUKA, MAMORU UEMURA, NAOTSUGU HARAGUCHI, JUNICHI NISHIMURA, ICHIRO TAKEMASA, TSUNEKAZU MIZUSHIMA, YUICHIRO DOKI and MASAKI MORI

Int J Oncol 42: 197–201, 2013; DOI: 10.3892/ijo.2012.1714

Following the publication of this article, an interested reader drew to our attention an anomaly associated with the presentation of the Fig. 2D; specifically, there appeared to have been a duplication of the same β-actin control band for the G4.4A GPI-M and the Absorbed Ab (antibody) experiments.

Figure 2 Western blot analysis for the C4.4A protein using lysates from HCT116 colon cancer cells. (A) C4.4A-119 antibody; (B) C4.4A-277 antibody; (C) C4.4A GPI-P antibody; and (D) C4.4A GPI-M antibody. The arrows indicate the band corresponding to the C4.4A protein. β-actin bands in the lower panels were produced from simultaneously performed western blot analyses using different gels or lanes with the same lysates from HCT116 cells.

After having re-examined our original data, we realize that the same β-actin control bands were inadvertently selected for the data shown in Fig. 2D, and this escaped out attention at the time. Our investigation of the data has also confirmed that the β-actin bands in the lower panels were produced from simultaneously performed blots using different gels or lanes with the same HCT116 cell lysates.

A corrected version of Fig. 2, containing alternative data obtained from an experiment performed in duplicate, is showin below. The error with the selection of the control data did not affect the results in this study. We sincerely apologize for this mistake, and thank the reader of our article who drew this matter to our attention. Furthermore, we regret any inconvenience this mistake has caused.

Figure 2 Western blot analysis for the C4.4A protein using lysates from HCT116 colon cancer cells. (A) C4.4A-119 antibody; (B) C4.4A-277 antibody; (C) C4.4A GPI-P antibody; and (D) C4.4A GPI-M antibody. The arrows indicate the band corresponding to the C4.4A protein. β-actin bands in the lower panels were produced from simultaneously performed western blot analyses using different gels or lanes with the same lysates from HCT116 cells.