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International Journal of Oncology
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December-2016 Volume 49 Issue 6

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Article

RING1 and YY1 binding protein suppresses breast cancer growth and metastasis

  • Authors:
    • Hongyan Zhou
    • Jie Li
    • Zhanqiang Zhang
    • Runyi Ye
    • Nan Shao
    • Tuckyun Cheang
    • Shenming Wang
  • View Affiliations / Copyright

    Affiliations: Department of Neurological Intensive Care Unit, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. China, Breast and Thyroid Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. China
  • Pages: 2442-2452
    |
    Published online on: October 5, 2016
       https://doi.org/10.3892/ijo.2016.3718
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Abstract

Evidence suggests that RING1 and YY1 binding protein (RYBP) functions as a tumor suppressor. However, its role in breast cancer remains unclear. In the present study, the expression of RYBP was assessed in breast cancer patients and cell lines. Disease-free survival durations of breast cancer patients with high RYBP expression were determined based on the ATCG dataset. The effects of RYBP overexpression on cell growth, migration and invasive potency were also assessed. Nude mouse xenograft and lung metastasis models were also used to confirm the role of RYBP. The involvement of SRRM3 in RYBP-mediated breast cancer suppression was explored using SRRM3 siRNA. The potential relationship between RYBP, SRRM3, and REST-003 was examined by qPCR. The results showed that RYBP was downregulated in breast cancer patients and in several breast cancer cell lines. Breast cancer patients with high expression levels of RYBP displayed better disease-free survival. Overexpression of RYBP in MDA-MB-231 and SK-BR-3 cells significantly decreased cell proliferation, migration, and invasion ability, and increased the proportion of cells arrested in S-phase compared with the negative control cells. Additionally, upregulation of proliferation-related cell cycle proteins (cyclin A and cyclin B1) and E-cadherin, and downregulation of snail were observed in RYBP-overexpressing cells. Overexpression of RYBP reduced tumor volume and weight as well as metastatic foci in the lungs of nude mice. SRRM3 knockdown by siRNA, which is downregulated after RYBP overexpression, suppressed cell growth and metastasis in MDA-MB-231 and SK-BR-3 cells. Furthermore, qPCR analysis revealed that REST-003 ncRNA was downregulated in cells overexpressing RYBP and in SRRM3-inhibited cells. Moreover, cell invasion ability and growth were increased after SRRM3 upregulation in RYBP-overexpressing cells, but they were decreased following si-REST-003 transfection. In conclusion, overexpression of RYBP suppresses breast cancer growth and metastasis both in vitro and in vivo. SRRM3 and REST-003, which are downregulated in cells overexpressing RYBP, may be involved in RYBP-mediated breast cancer progression.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou H, Li J, Zhang Z, Ye R, Shao N, Cheang T and Wang S: RING1 and YY1 binding protein suppresses breast cancer growth and metastasis. Int J Oncol 49: 2442-2452, 2016.
APA
Zhou, H., Li, J., Zhang, Z., Ye, R., Shao, N., Cheang, T., & Wang, S. (2016). RING1 and YY1 binding protein suppresses breast cancer growth and metastasis. International Journal of Oncology, 49, 2442-2452. https://doi.org/10.3892/ijo.2016.3718
MLA
Zhou, H., Li, J., Zhang, Z., Ye, R., Shao, N., Cheang, T., Wang, S."RING1 and YY1 binding protein suppresses breast cancer growth and metastasis". International Journal of Oncology 49.6 (2016): 2442-2452.
Chicago
Zhou, H., Li, J., Zhang, Z., Ye, R., Shao, N., Cheang, T., Wang, S."RING1 and YY1 binding protein suppresses breast cancer growth and metastasis". International Journal of Oncology 49, no. 6 (2016): 2442-2452. https://doi.org/10.3892/ijo.2016.3718
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou H, Li J, Zhang Z, Ye R, Shao N, Cheang T and Wang S: RING1 and YY1 binding protein suppresses breast cancer growth and metastasis. Int J Oncol 49: 2442-2452, 2016.
APA
Zhou, H., Li, J., Zhang, Z., Ye, R., Shao, N., Cheang, T., & Wang, S. (2016). RING1 and YY1 binding protein suppresses breast cancer growth and metastasis. International Journal of Oncology, 49, 2442-2452. https://doi.org/10.3892/ijo.2016.3718
MLA
Zhou, H., Li, J., Zhang, Z., Ye, R., Shao, N., Cheang, T., Wang, S."RING1 and YY1 binding protein suppresses breast cancer growth and metastasis". International Journal of Oncology 49.6 (2016): 2442-2452.
Chicago
Zhou, H., Li, J., Zhang, Z., Ye, R., Shao, N., Cheang, T., Wang, S."RING1 and YY1 binding protein suppresses breast cancer growth and metastasis". International Journal of Oncology 49, no. 6 (2016): 2442-2452. https://doi.org/10.3892/ijo.2016.3718
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