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Article

Detection of cancer specific mutations in early-stage non-small cell lung cancer using cell-free DNA by targeted sequencing

  • Authors:
    • Yutong He
    • Xinyuan Zhang
    • Liqun Wang
    • Ziqiang Tian
    • Qingyi Liu
    • Jifang Yao
    • Yueping Liu
    • Chuanbao Li
    • Li Min
    • Baoen Shan
  • View Affiliations / Copyright

    Affiliations: The Fourth Hospital of Hebei Medical University/The Tumor Hospital of Hebei Province, Shijiazhuang, Hebei 050011, P.R. China, Institute of Genecrab Precision Medicine, Beijing 100078, P.R. China, Department of Clinical Laboratory, Beijing Hospital, Beijing 100730, P.R. China, Department of Biochemistry and Molecular Biology, Peking University Cancer Hospital and Institute, Beijing 100142, P.R. China
  • Pages: 2351-2358
    |
    Published online on: October 12, 2016
       https://doi.org/10.3892/ijo.2016.3731
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Abstract

Non-small cell lung cancer (NSCLC) is a major public health problem worldwide and leads to a high mortality. NSCLC is always diagnosed in late stages because of its unapparent symptoms. However, cell-free DNA (cfDNA) may serve as a new potential biomarker to detect early stage of non‑small cell lung cancer. Here we recruited 10 non-small cell lung cancer patients to obtain fresh tumor tissue, peripheral blood lymphocytes (PBLs), and plasma. CfDNAs from 13 elderly people and 7 middle-age smokers were also extracted as controls. Illumina HiSeq X10 was used to perform next-generation sequencing to evaluate differences in mutations among different samples. The result indicated that tumor DNA and its matched plasma cfDNA samples showed high concordance in their mutation patterns. Mutation rate of cfDNA was generally lower than that of tumor tissue and higher than that of PBLs. The plasma cfDNA concentration of NSCLC patients (69.2±46.9 ng/ml) is significantly higher than that of elderly people (32.5±5.2 ng/ml, t=2.96, p=0.007) and middle-aged smokers (17.9±9.1 ng/ml, t=2.83, p=0.013). Five mutations (PTEN_c.1375A>G, TP53_c.94G>A, STK11_c.816C>T, PIK3CA_c.1633 G>A, PIK3CA_c.2038G>C) were only identified in NSCLC patients but not in healthy people. Our conclusion was that cfDNA has a similar mutation pattern with its matched tumor tissue DNA. A high concentration of cfDNA and tumor specific mutations in cfDNA may serve as potential non-invasive biomarkers to detect early-stage non‑small cell lung cancer.
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Copy and paste a formatted citation
Spandidos Publications style
He Y, Zhang X, Wang L, Tian Z, Liu Q, Yao J, Liu Y, Li C, Min L, Shan B, Shan B, et al: Detection of cancer specific mutations in early-stage non-small cell lung cancer using cell-free DNA by targeted sequencing. Int J Oncol 49: 2351-2358, 2016.
APA
He, Y., Zhang, X., Wang, L., Tian, Z., Liu, Q., Yao, J. ... Shan, B. (2016). Detection of cancer specific mutations in early-stage non-small cell lung cancer using cell-free DNA by targeted sequencing. International Journal of Oncology, 49, 2351-2358. https://doi.org/10.3892/ijo.2016.3731
MLA
He, Y., Zhang, X., Wang, L., Tian, Z., Liu, Q., Yao, J., Liu, Y., Li, C., Min, L., Shan, B."Detection of cancer specific mutations in early-stage non-small cell lung cancer using cell-free DNA by targeted sequencing". International Journal of Oncology 49.6 (2016): 2351-2358.
Chicago
He, Y., Zhang, X., Wang, L., Tian, Z., Liu, Q., Yao, J., Liu, Y., Li, C., Min, L., Shan, B."Detection of cancer specific mutations in early-stage non-small cell lung cancer using cell-free DNA by targeted sequencing". International Journal of Oncology 49, no. 6 (2016): 2351-2358. https://doi.org/10.3892/ijo.2016.3731
Copy and paste a formatted citation
x
Spandidos Publications style
He Y, Zhang X, Wang L, Tian Z, Liu Q, Yao J, Liu Y, Li C, Min L, Shan B, Shan B, et al: Detection of cancer specific mutations in early-stage non-small cell lung cancer using cell-free DNA by targeted sequencing. Int J Oncol 49: 2351-2358, 2016.
APA
He, Y., Zhang, X., Wang, L., Tian, Z., Liu, Q., Yao, J. ... Shan, B. (2016). Detection of cancer specific mutations in early-stage non-small cell lung cancer using cell-free DNA by targeted sequencing. International Journal of Oncology, 49, 2351-2358. https://doi.org/10.3892/ijo.2016.3731
MLA
He, Y., Zhang, X., Wang, L., Tian, Z., Liu, Q., Yao, J., Liu, Y., Li, C., Min, L., Shan, B."Detection of cancer specific mutations in early-stage non-small cell lung cancer using cell-free DNA by targeted sequencing". International Journal of Oncology 49.6 (2016): 2351-2358.
Chicago
He, Y., Zhang, X., Wang, L., Tian, Z., Liu, Q., Yao, J., Liu, Y., Li, C., Min, L., Shan, B."Detection of cancer specific mutations in early-stage non-small cell lung cancer using cell-free DNA by targeted sequencing". International Journal of Oncology 49, no. 6 (2016): 2351-2358. https://doi.org/10.3892/ijo.2016.3731
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