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Metabolomic profiling of breast tumors using ductal fluid

  • Authors:
    • Luisa Matos Do Canto
    • Catalin Marian
    • Rency S. Varghese
    • Jaeil Ahn
    • Patricia A. Da Cunha
    • Shawna Willey
    • Mary Sidawy
    • Janice D. Rone
    • Amrita K. Cheema
    • George Luta
    • Mohammad R. Nezami Ranjbar
    • Habtom W. Ressom
    • Bassem R. Haddad
  • View Affiliations / Copyright

    Affiliations: Lombardi Comprehensive Cancer Center and Department of Oncology, Georgetown University Medical Center, Georgetown University, Washington DC, USA, Biochemistry Department, ‘Victor Babes’ University of Medicine and Pharmacy, Timisoara, Romania
    Copyright: © Matos Do Canto et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2245-2254
    |
    Published online on: October 13, 2016
       https://doi.org/10.3892/ijo.2016.3732
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Abstract

Identification of new biomarkers for breast cancer remains critical in order to enhance early detection of the disease and improve its prognosis. Towards this end, we performed an untargeted metabolomic analysis of breast ductal fluid using an ultra-performance liquid chromatography coupled with a quadrupole time-of-light (UPLC-QTOF) mass spectrometer. We investigated the metabolomic profiles of breast tumors using ductal fluid samples collected by ductal lavage (DL). We studied fluid from both the affected breasts and the unaffected contralateral breasts (as controls) from 43 women with confirmed unilateral breast cancer. Using this approach, we identified 1560 ions in the positive mode and 538 ions in the negative mode after preprocessing of the UPLC‑QTOF data. Paired t-tests applied on these data matrices identified 209 ions (positive and negative modes combined) with significant change in intensity level between affected and unaffected control breasts (adjusted p-values <0.05). Among these, 83 ions (39.7%) showed a fold change (FC) >1.2 and 66 ions (31.6%) were identified with putative compound names. The metabolites that we identified included endogenous metabolites such as amino acid derivatives (N-Acetyl-DL-tryptophan) or products of lipid metabolism such as N-linoleoyl taurine, trans-2-dodecenoylcarnitine, lysophosphatidylcholine LysoPC(18:2(9Z,12Z)), glycerophospholipids PG(18:0/0:0), and phosphatidylserine PS(20:4(5Z,8Z,11Z,14Z). Generalized LASSO regression further selected 21 metabolites when race, menopausal status, smoking, grade and TNM stage were adjusted for. A predictive conditional logistic regression model, using the LASSO selected 21 ions, provided diagnostic accuracy with the area under the curve of 0.956 (sensitivity/specificity of 0.907/0.884). This is the first study that shows the feasibility of conducting a comprehensive metabolomic profiling of breast tumors using breast ductal fluid to detect changes in the cellular microenvironment of the tumors and shows the potential for this approach to be used to improve detection of breast cancer.
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View References

1 

American Cancer Society. Global Cancer Facts and Figures. 3rd edition. American Cancer Society; Atlanta, GA: 2015

2 

Warburg O: On the origin of cancer cells. Science. 123:309–314. 1956. View Article : Google Scholar : PubMed/NCBI

3 

Denkert C, Bucher E, Hilvo M, Salek R, Orešič M, Griffin J, Brockmöller S, Klauschen F, Loibl S, Barupal DK, et al: Metabolomics of human breast cancer: New approaches for tumor typing and biomarker discovery. Genome Med. 4:372012.PubMed/NCBI

4 

Sengupta D and Pratx G: Imaging metabolic heterogeneity in cancer. Mol Cancer. 15:42016. View Article : Google Scholar : PubMed/NCBI

5 

Long JP, Li XN and Zhang F: Targeting metabolism in breast cancer: How far we can go? World J Clin Oncol. 7:122–130. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Mannello F and Ligi D: Resolving breast cancer heterogeneity by searching reliable protein cancer biomarkers in the breast fluid secretome. BMC Cancer. 13:3442013. View Article : Google Scholar : PubMed/NCBI

7 

Shajahan-Haq AN, Cheema MS and Clarke R: Application of metabolomics in drug resistant breast cancer research. Metabolites. 5:100–118. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Claudino WM, Quattrone A, Biganzoli L, Pestrin M, Bertini I and Di Leo A: Metabolomics: Available results, current research projects in breast cancer, and future applications. J Clin Oncol. 25:2840–2846. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Wang J, Zuo Y, Man YG, Avital I, Stojadinovic A, Liu M, Yang X, Varghese RS, Tadesse MG and Ressom HW: Pathway and network approaches for identification of cancer signature markers from omics data. J Cancer. 6:54–65. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Sitter B, Lundgren S, Bathen TF, Halgunset J, Fjosne HE and Gribbestad IS: Comparison of HR MAS MR spectroscopic profiles of breast cancer tissue with clinical parameters. NMR Biomed. 19:30–40. 2006. View Article : Google Scholar

11 

Mountford CE, Somorjai RL, Malycha P, Gluch L, Lean C, Russell P, Barraclough B, Gillett D, Himmelreich U, Dolenko B, et al: Diagnosis and prognosis of breast cancer by magnetic resonance spectroscopy of fine-needle aspirates analysed using a statistical classification strategy. Br J Surg. 88:1234–1240. 2001. View Article : Google Scholar : PubMed/NCBI

12 

Budczies J, Denkert C, Müller BM, Brockmöller SF, Klauschen F, Györffy B, Dietel M, Richter-Ehrenstein C, Marten U, Salek RM, et al: Remodeling of central metabolism in invasive breast cancer compared to normal breast tissue - a GC-TOFMS based metabolomics study. BMC Genomics. 13:3342012. View Article : Google Scholar : PubMed/NCBI

13 

Budczies J, Brockmöller SF, Müller BM, Barupal DK, Richter-Ehrenstein C, Kleine-Tebbe A, Griffin JL, Orešič M, Dietel M, Denkert C, et al: Comparative metabolomics of estrogen receptor positive and estrogen receptor negative breast cancer: Alterations in glutamine and beta-alanine metabolism. J Proteomics. 94:279–288. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Jobard E, Pontoizeau C, Blaise BJ, Bachelot T, Elena-Herrmann B and Trédan O: A serum nuclear magnetic resonance-based metabolomic signature of advanced metastatic human breast cancer. Cancer Lett. 343:33–41. 2014. View Article : Google Scholar

15 

Asiago VM, Alvarado LZ, Shanaiah N, Gowda GA, Owusu-Sarfo K, Ballas RA and Raftery D: Early detection of recurrent breast cancer using metabolite profiling. Cancer Res. 70:8309–8318. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Budhu A, Terunuma A, Zhang G, Hussain SP, Ambs S and Wang XW: Metabolic profiles are principally different between cancers of the liver, pancreas and breast. Int J Biol Sci. 10:966–972. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Armitage EG and Barbas C: Metabolomics in cancer biomarker discovery: Current trends and future perspectives. J Pharm Biomed Anal. 87:1–11. 2014. View Article : Google Scholar

18 

Tredwell GD, Miller JA, Chow HH, Thompson PA and Keun HC: Metabolomic characterization of nipple aspirate fluid by 1H NMR spectroscopy and GC-MS. J Proteome Res. 13:883–889. 2014. View Article : Google Scholar

19 

Do Canto LM, Marian C, Willey S, Sidawy M, Da Cunha PA, Rone JD, Li X, Gusev Y and Haddad BR: MicroRNA analysis of breast ductal fluid in breast cancer patients. Int J Oncol. 48:2071–2078. 2016.PubMed/NCBI

20 

Isaacs C, Cavalli LR, Cohen Y, Pennanen M, Shankar LK, Freedman M, Singh B, Liu M, Gallagher A, Rone JD, et al: Detection of LOH and mitochondrial DNA alterations in ductal lavage and nipple aspirate fluids from high-risk patients. Breast Cancer Res Treat. 84:99–105. 2004. View Article : Google Scholar : PubMed/NCBI

21 

Masood S: Development of a novel approach for breast cancer prediction and early detection using minimally invasive procedures and molecular analysis: How cytomorphology became a breast cancer risk predictor. Breast J. 21:82–96. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Sheikh KD, Khanna S, Byers SW, Fornace A Jr and Cheema AK: Small molecule metabolite extraction strategy for improving LC/MS detection of cancer cell metabolome. J Biomol Tech. 22:1–4. 2011.PubMed/NCBI

23 

Kaur P, Rizk N, Ibrahim S, Luo Y, Younes N, Perry B, Dennis K, Zirie M, Luta G and Cheema AK: Quantitative metabolomic and lipidomic profiling reveals aberrant amino acid metabolism in type 2 diabetes. Mol Biosyst. 9:307–317. 2013. View Article : Google Scholar

24 

Patterson AD, Li H, Eichler GS, Krausz KW, Weinstein JN, Fornace AJ Jr, Gonzalez FJ and Idle JR: UPLC-ESI-TOFMS-based metabolomics and gene expression dynamics inspector self-organizing metabolomic maps as tools for understanding the cellular response to ionizing radiation. Anal Chem. 80:665–674. 2008. View Article : Google Scholar : PubMed/NCBI

25 

Kuhl C, Tautenhahn R, Böttcher C, Larson TR and Neumann S: CAMERA: An integrated strategy for compound spectra extraction and annotation of liquid chromatography/mass spectrometry data sets. Anal Chem. 84:283–289. 2012. View Article : Google Scholar

26 

Storey J: False discovery rates. International Encyclopedia of Statistical Science. 1st edition. Lovric M: Springer; pp. 16732011

27 

Westerhuis JA, van Velzen EJ, Hoefsloot HC and Smilde AK: Multivariate paired data analysis: Multilevel PLSDA versus OPLSDA. Metabolomics. 6:119–128. 2010. View Article : Google Scholar : PubMed/NCBI

28 

Friedman J, Hastie T and Tibshirani R: Regularization paths for generalized linear models via coordinate descent. J Stat Softw. 33:1–22. 2010. View Article : Google Scholar : PubMed/NCBI

29 

Reid S and Tibshirani R: Regularization paths for conditional logistic regression: The clogitL1 package. J Stat Softw. 58:122014. View Article : Google Scholar

30 

Zhou B, Wang J and Ressom HW: MetaboSearch: Tool for mass-based metabolite identification using multiple databases. PLoS One. 7:e400962012. View Article : Google Scholar : PubMed/NCBI

31 

Wishart DS, Knox C, Guo AC, Eisner R, Young N, Gautam B, Hau DD, Psychogios N, Dong E, Bouatra S, et al: HMDB: A knowledgebase for the human metabolome. Nucleic Acids Res. 37(Database): D603–D610. 2009. View Article : Google Scholar :

32 

Smith CA, O'Maille G, Want EJ, Qin C, Trauger SA, Brandon TR, Custodio DE, Abagyan R and Siuzdak G: METLIN: A metabolite mass spectral database. Ther Drug Monit. 27:747–751. 2005. View Article : Google Scholar

33 

Cui Q, Lewis IA, Hegeman AD, Anderson ME, Li J, Schulte CF, Westler WM, Eghbalnia HR, Sussman MR and Markley JL: Metabolite identification via the Madison Metabolomics Consortium Database. Nat Biotechnol. 26:162–164. 2008. View Article : Google Scholar : PubMed/NCBI

34 

Sud M, Fahy E, Cotter D, Brown A, Dennis EA, Glass CK, Merrill AH Jr, Murphy RC, Raetz CR, Russell DW, et al: LMSD: LIPID MAPS structure database. Nucleic Acids Res. 35(Database): D527–D532. 2007. View Article : Google Scholar

35 

Fan Y, Zhou X, Xia TS, Chen Z, Li J, Liu Q, Alolga RN, Chen Y, Lai MD, Li P, et al: Human plasma metabolomics for identifying differential metabolites and predicting molecular subtypes of breast cancer. Oncotarget. 7:9925–9938. 2016.PubMed/NCBI

36 

Mishra P and Ambs S: Metabolic signatures of human breast cancer. Mol Cell Oncol. 2:22015.

37 

Qiu Y, Zhou B, Su M, Baxter S, Zheng X, Zhao X, Yen Y and Jia W: Mass spectrometry-based quantitative metabolomics revealed a distinct lipid profile in breast cancer patients. Int J Mol Sci. 14:8047–8061. 2013. View Article : Google Scholar : PubMed/NCBI

38 

Shen J, Yan L, Liu S, Ambrosone CB and Zhao H: Plasma metabolomic profiles in breast cancer patients and healthy controls: By race and tumor receptor subtypes. Transl Oncol. 6:757–765. 2013. View Article : Google Scholar

39 

Willmann L, Schlimpert M, Halbach S, Erbes T, Stickeler E and Kammerer B: Metabolic profiling of breast cancer: Differences in central metabolism between subtypes of breast cancer cell lines. J Chromatogr B Analyt Technol Biomed Life Sci. 1000:95–104. 2015. View Article : Google Scholar : PubMed/NCBI

40 

Yamashita J, Ogawa M and Sakai K: Prognostic significance of three novel biologic factors in a clinical trial of adjuvant therapy for node-negative breast cancer. Surgery. 117:601–608. 1995. View Article : Google Scholar : PubMed/NCBI

41 

Yamashita S, Yamashita J, Sakamoto K, Inada K, Nakashima Y, Murata K, Saishoji T, Nomura K and Ogawa M: Increased expression of membrane-associated phospholipase A2 shows malignant potential of human breast cancer cells. Cancer. 71:3058–3064. 1993. View Article : Google Scholar : PubMed/NCBI

42 

Takayama T, Tsutsui H, Shimizu I, Toyama T, Yoshimoto N, Endo Y, Inoue K, Todoroki K, Min JZ, Mizuno H, et al: Diagnostic approach to breast cancer patients based on target metabolomics in saliva by liquid chromatography with tandem mass spectrometry. Clin Chim Acta. 452:18–26. 2016. View Article : Google Scholar

43 

Hadi NI and Jamal Q: ‘OMIC’ tumor markers for breast cancer: A review. Pak J Med Sci. 31:1256–1262. 2015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Matos Do Canto L, Marian C, Varghese RS, Ahn J, Da Cunha PA, Willey S, Sidawy M, Rone JD, Cheema AK, Luta G, Luta G, et al: Metabolomic profiling of breast tumors using ductal fluid. Int J Oncol 49: 2245-2254, 2016.
APA
Matos Do Canto, L., Marian, C., Varghese, R.S., Ahn, J., Da Cunha, P.A., Willey, S. ... Haddad, B.R. (2016). Metabolomic profiling of breast tumors using ductal fluid. International Journal of Oncology, 49, 2245-2254. https://doi.org/10.3892/ijo.2016.3732
MLA
Matos Do Canto, L., Marian, C., Varghese, R. S., Ahn, J., Da Cunha, P. A., Willey, S., Sidawy, M., Rone, J. D., Cheema, A. K., Luta, G., Nezami Ranjbar, M. R., Ressom, H. W., Haddad, B. R."Metabolomic profiling of breast tumors using ductal fluid". International Journal of Oncology 49.6 (2016): 2245-2254.
Chicago
Matos Do Canto, L., Marian, C., Varghese, R. S., Ahn, J., Da Cunha, P. A., Willey, S., Sidawy, M., Rone, J. D., Cheema, A. K., Luta, G., Nezami Ranjbar, M. R., Ressom, H. W., Haddad, B. R."Metabolomic profiling of breast tumors using ductal fluid". International Journal of Oncology 49, no. 6 (2016): 2245-2254. https://doi.org/10.3892/ijo.2016.3732
Copy and paste a formatted citation
x
Spandidos Publications style
Matos Do Canto L, Marian C, Varghese RS, Ahn J, Da Cunha PA, Willey S, Sidawy M, Rone JD, Cheema AK, Luta G, Luta G, et al: Metabolomic profiling of breast tumors using ductal fluid. Int J Oncol 49: 2245-2254, 2016.
APA
Matos Do Canto, L., Marian, C., Varghese, R.S., Ahn, J., Da Cunha, P.A., Willey, S. ... Haddad, B.R. (2016). Metabolomic profiling of breast tumors using ductal fluid. International Journal of Oncology, 49, 2245-2254. https://doi.org/10.3892/ijo.2016.3732
MLA
Matos Do Canto, L., Marian, C., Varghese, R. S., Ahn, J., Da Cunha, P. A., Willey, S., Sidawy, M., Rone, J. D., Cheema, A. K., Luta, G., Nezami Ranjbar, M. R., Ressom, H. W., Haddad, B. R."Metabolomic profiling of breast tumors using ductal fluid". International Journal of Oncology 49.6 (2016): 2245-2254.
Chicago
Matos Do Canto, L., Marian, C., Varghese, R. S., Ahn, J., Da Cunha, P. A., Willey, S., Sidawy, M., Rone, J. D., Cheema, A. K., Luta, G., Nezami Ranjbar, M. R., Ressom, H. W., Haddad, B. R."Metabolomic profiling of breast tumors using ductal fluid". International Journal of Oncology 49, no. 6 (2016): 2245-2254. https://doi.org/10.3892/ijo.2016.3732
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