IMP-3 protects the mRNAs of cyclins D1 and D3 from GW182/AGO2-dependent translational repression

  • Authors:
    • Evgeny Deforzh
    • Thaiz Rivera Vargas
    • Jeremie Kropp
    • Marie Vandamme
    • Guillaume Pinna
    • Anna Polesskaya
  • View Affiliations

  • Published online on: October 26, 2016     https://doi.org/10.3892/ijo.2016.3750
  • Pages:2578-2588
0

Abstract

IGF-2 mRNA binding protein 3 (IGF2BP3, IMP-3) is a well-known post-transcriptional regulatory factor of gene expression, mainly involved in embryonic development and oncogenesis. We have previously demonstrated that a subset of IMP-3 targets, such as the mRNAs of cyclins D1, D3 and G1, are positively regulated by IMP-3, and that this regulation depends on nuclear localization of IMP-3. In the present study, we show that as a first step following a knock-down of IMP-3, the protein levels of the cyclins rapidly decrease, while their mRNAs remain stable and associated with the polyribosomes, though not translated. We have elucidated the molecular mechanisms of this regulation, demonstrating that IMP-3 and its protein partners ILF3/NF90 and PTBP1 bind to the 3'UTRs of the cyclin mRNAs and protect them from the translational repression induced by miRNA-dependent recruitment of AGO2/GW182 complex in human cancer cells.

Related Articles

Journal Cover

December 2016
Volume 49 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

2016 Impact Factor: 3.079
Ranked #33/217 Oncology
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Deforzh, E., Vargas, T.R., Kropp, J., Vandamme, M., Pinna, G., & Polesskaya, A. (2016). IMP-3 protects the mRNAs of cyclins D1 and D3 from GW182/AGO2-dependent translational repression. International Journal of Oncology, 49, 2578-2588. https://doi.org/10.3892/ijo.2016.3750
MLA
Deforzh, E., Vargas, T. R., Kropp, J., Vandamme, M., Pinna, G., Polesskaya, A."IMP-3 protects the mRNAs of cyclins D1 and D3 from GW182/AGO2-dependent translational repression". International Journal of Oncology 49.6 (2016): 2578-2588.
Chicago
Deforzh, E., Vargas, T. R., Kropp, J., Vandamme, M., Pinna, G., Polesskaya, A."IMP-3 protects the mRNAs of cyclins D1 and D3 from GW182/AGO2-dependent translational repression". International Journal of Oncology 49, no. 6 (2016): 2578-2588. https://doi.org/10.3892/ijo.2016.3750