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International Journal of Oncology
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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Article

IMP-3 protects the mRNAs of cyclins D1 and D3 from GW182/AGO2-dependent translational repression

  • Authors:
    • Evgeny Deforzh
    • Thaiz Rivera Vargas
    • Jeremie Kropp
    • Marie Vandamme
    • Guillaume Pinna
    • Anna Polesskaya
  • View Affiliations / Copyright

    Affiliations: Institute for Integrative Biology of the Cell (I2BC), IBITECS, CEA, CNRS, University Paris XI, University Paris-Saclay, 91198, Gif-sur-Yvette cedex, France, INSERM, UMR 866, Centre Georges François Leclerc, University of Bourgogne, Dijon, France
  • Pages: 2578-2588
    |
    Published online on: October 26, 2016
       https://doi.org/10.3892/ijo.2016.3750
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Abstract

IGF-2 mRNA binding protein 3 (IGF2BP3, IMP-3) is a well-known post-transcriptional regulatory factor of gene expression, mainly involved in embryonic development and oncogenesis. We have previously demonstrated that a subset of IMP-3 targets, such as the mRNAs of cyclins D1, D3 and G1, are positively regulated by IMP-3, and that this regulation depends on nuclear localization of IMP-3. In the present study, we show that as a first step following a knock-down of IMP-3, the protein levels of the cyclins rapidly decrease, while their mRNAs remain stable and associated with the polyribosomes, though not translated. We have elucidated the molecular mechanisms of this regulation, demonstrating that IMP-3 and its protein partners ILF3/NF90 and PTBP1 bind to the 3'UTRs of the cyclin mRNAs and protect them from the translational repression induced by miRNA-dependent recruitment of AGO2/GW182 complex in human cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
Deforzh E, Vargas TR, Kropp J, Vandamme M, Pinna G and Polesskaya A: IMP-3 protects the mRNAs of cyclins D1 and D3 from GW182/AGO2-dependent translational repression. Int J Oncol 49: 2578-2588, 2016.
APA
Deforzh, E., Vargas, T.R., Kropp, J., Vandamme, M., Pinna, G., & Polesskaya, A. (2016). IMP-3 protects the mRNAs of cyclins D1 and D3 from GW182/AGO2-dependent translational repression. International Journal of Oncology, 49, 2578-2588. https://doi.org/10.3892/ijo.2016.3750
MLA
Deforzh, E., Vargas, T. R., Kropp, J., Vandamme, M., Pinna, G., Polesskaya, A."IMP-3 protects the mRNAs of cyclins D1 and D3 from GW182/AGO2-dependent translational repression". International Journal of Oncology 49.6 (2016): 2578-2588.
Chicago
Deforzh, E., Vargas, T. R., Kropp, J., Vandamme, M., Pinna, G., Polesskaya, A."IMP-3 protects the mRNAs of cyclins D1 and D3 from GW182/AGO2-dependent translational repression". International Journal of Oncology 49, no. 6 (2016): 2578-2588. https://doi.org/10.3892/ijo.2016.3750
Copy and paste a formatted citation
x
Spandidos Publications style
Deforzh E, Vargas TR, Kropp J, Vandamme M, Pinna G and Polesskaya A: IMP-3 protects the mRNAs of cyclins D1 and D3 from GW182/AGO2-dependent translational repression. Int J Oncol 49: 2578-2588, 2016.
APA
Deforzh, E., Vargas, T.R., Kropp, J., Vandamme, M., Pinna, G., & Polesskaya, A. (2016). IMP-3 protects the mRNAs of cyclins D1 and D3 from GW182/AGO2-dependent translational repression. International Journal of Oncology, 49, 2578-2588. https://doi.org/10.3892/ijo.2016.3750
MLA
Deforzh, E., Vargas, T. R., Kropp, J., Vandamme, M., Pinna, G., Polesskaya, A."IMP-3 protects the mRNAs of cyclins D1 and D3 from GW182/AGO2-dependent translational repression". International Journal of Oncology 49.6 (2016): 2578-2588.
Chicago
Deforzh, E., Vargas, T. R., Kropp, J., Vandamme, M., Pinna, G., Polesskaya, A."IMP-3 protects the mRNAs of cyclins D1 and D3 from GW182/AGO2-dependent translational repression". International Journal of Oncology 49, no. 6 (2016): 2578-2588. https://doi.org/10.3892/ijo.2016.3750
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