IMP-3 protects the mRNAs of cyclins D1 and D3 from GW182/AGO2-dependent translational repression

Deforzh,Evgeny; Vargas,Thaiz Rivera; Kropp,Jeremie; Vandamme,Marie; Pinna,Guillaume; Polesskaya,Anna

doi:10.3892/ijo.2016.3750

IMP-3 protects the mRNAs of cyclins D1 and D3 from GW182/AGO2-dependent translational repression

Authors:
- Evgeny Deforzh
- Thaiz Rivera Vargas
- Jeremie Kropp
- Marie Vandamme
- Guillaume Pinna
- Anna Polesskaya
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Affiliations: Institute for Integrative Biology of the Cell (I2BC), IBITECS, CEA, CNRS, University Paris XI, University Paris-Saclay, 91198, Gif-sur-Yvette cedex, France, INSERM, UMR 866, Centre Georges François Leclerc, University of Bourgogne, Dijon, France
Published online on: October 26, 2016 https://doi.org/10.3892/ijo.2016.3750
Pages: 2578-2588

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Abstract

IGF-2 mRNA binding protein 3 (IGF2BP3, IMP-3) is a well-known post-transcriptional regulatory factor of gene expression, mainly involved in embryonic development and oncogenesis. We have previously demonstrated that a subset of IMP-3 targets, such as the mRNAs of cyclins D1, D3 and G1, are positively regulated by IMP-3, and that this regulation depends on nuclear localization of IMP-3. In the present study, we show that as a first step following a knock-down of IMP-3, the protein levels of the cyclins rapidly decrease, while their mRNAs remain stable and associated with the polyribosomes, though not translated. We have elucidated the molecular mechanisms of this regulation, demonstrating that IMP-3 and its protein partners ILF3/NF90 and PTBP1 bind to the 3'UTRs of the cyclin mRNAs and protect them from the translational repression induced by miRNA-dependent recruitment of AGO2/GW182 complex in human cancer cells.

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