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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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Article

The multi-target drug BAI induces apoptosis in various human cancer cells through modulation of Bcl-xL protein

  • Authors:
    • Shin Kim
    • Dong Eun Kim
    • Taeg Kyu Kwon
    • Jinho Lee
    • Jong-Wook Park
  • View Affiliations / Copyright

    Affiliations: Department of Immunology, School of Medicine, Keimyung University, Daegu 42601, Republic of Korea, Department of Chemistry, Keimyung University, Daegu 42601, Republic of Korea
  • Pages: 2620-2628
    |
    Published online on: November 3, 2016
       https://doi.org/10.3892/ijo.2016.3758
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Abstract

Previous studies have demonstrated the anticancer effects of the newly developed cyclin-dependent kinase inhibitor BAI in various cancer cells. However, the molecular mechanisms of the cellular effects induced by BAI have not been fully elucidated. The objective of this study was to investigate the mechanisms underlying the regulation of B cell lymphoma-2 (Bcl-2) family proteins in BAI-induced apoptosis of cancer cells. BAI induced poly(ADP-ribose) polymerase cleavage and DEVDase activation dose- and time-dependently. However, BAI-induced apoptosis was not involved in reactive oxygen species generation or mitogen-activated protein kinases pathways. On the other hand, BAI reduced the mitochondrial membrane potential (∆ψm) dose- and time-dependently, and induced the release of apoptosis-inducing factor (AIF) and cytochrome c from mitochondria in A549 and Caki cells. Furthermore, BAI-induced apoptosis was strongly associated with downregulation of B-cell lymphoma-extra large (Bcl-xL), but not Bcl-2, and BAI modulated the interactions among p53 and Bcl-2 family proteins in human cancer cells. Taken together, these results revealed that the regulations of Bcl-2 family proteins are correlated with BAI-induced apoptosis, suggesting that BAI is a potential multi-target agent of cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Kim S, Kim DE, Kwon TK, Lee J and Park J: The multi-target drug BAI induces apoptosis in various human cancer cells through modulation of Bcl-xL protein. Int J Oncol 49: 2620-2628, 2016.
APA
Kim, S., Kim, D.E., Kwon, T.K., Lee, J., & Park, J. (2016). The multi-target drug BAI induces apoptosis in various human cancer cells through modulation of Bcl-xL protein. International Journal of Oncology, 49, 2620-2628. https://doi.org/10.3892/ijo.2016.3758
MLA
Kim, S., Kim, D. E., Kwon, T. K., Lee, J., Park, J."The multi-target drug BAI induces apoptosis in various human cancer cells through modulation of Bcl-xL protein". International Journal of Oncology 49.6 (2016): 2620-2628.
Chicago
Kim, S., Kim, D. E., Kwon, T. K., Lee, J., Park, J."The multi-target drug BAI induces apoptosis in various human cancer cells through modulation of Bcl-xL protein". International Journal of Oncology 49, no. 6 (2016): 2620-2628. https://doi.org/10.3892/ijo.2016.3758
Copy and paste a formatted citation
x
Spandidos Publications style
Kim S, Kim DE, Kwon TK, Lee J and Park J: The multi-target drug BAI induces apoptosis in various human cancer cells through modulation of Bcl-xL protein. Int J Oncol 49: 2620-2628, 2016.
APA
Kim, S., Kim, D.E., Kwon, T.K., Lee, J., & Park, J. (2016). The multi-target drug BAI induces apoptosis in various human cancer cells through modulation of Bcl-xL protein. International Journal of Oncology, 49, 2620-2628. https://doi.org/10.3892/ijo.2016.3758
MLA
Kim, S., Kim, D. E., Kwon, T. K., Lee, J., Park, J."The multi-target drug BAI induces apoptosis in various human cancer cells through modulation of Bcl-xL protein". International Journal of Oncology 49.6 (2016): 2620-2628.
Chicago
Kim, S., Kim, D. E., Kwon, T. K., Lee, J., Park, J."The multi-target drug BAI induces apoptosis in various human cancer cells through modulation of Bcl-xL protein". International Journal of Oncology 49, no. 6 (2016): 2620-2628. https://doi.org/10.3892/ijo.2016.3758
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