Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Oncology
Join Editorial Board Propose a Special Issue
Print ISSN: 1019-6439 Online ISSN: 1791-2423
Journal Cover
December-2016 Volume 49 Issue 6

Full Size Image

Cover Legend PDF

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2016 Volume 49 Issue 6

Full Size Image

Cover Legend PDF

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Expression of metallothionein 3 in ductal breast cancer

  • Authors:
    • Agnieszka Gomulkiewicz
    • Karolina Jablonska
    • Bartosz Pula
    • Jedrzej Grzegrzolka
    • Sylwia Borska
    • Marzenna Podhorska-Okolow
    • Andrzej Wojnar
    • Janusz Rys
    • Aleksandra Ambicka
    • Maciej Ugorski
    • Maciej Zabel
    • Piotr Dziegiel
  • View Affiliations / Copyright

    Affiliations: Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland, Department of Pathomorphology, Lower Silesian Oncology Centre, Wroclaw, Poland, Department of Tumor Pathology, Centre of Oncology, Maria Sklodowska-Curie Memorial Institute, Cracow Branch, Cracow, Poland, Department of Biochemistry, Pharmacology and Toxicology, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland
  • Pages: 2487-2497
    |
    Published online on: November 4, 2016
       https://doi.org/10.3892/ijo.2016.3759
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Metallothionein 3 (MT-3) has the ability to regulate the growth of nerve cells, but the significance of MT-3 expression outside the central nervous system and its participation in carcinogenesis have not yet been clarified. The aim of our study was to investigate the expression of MT-3 in ductal breast cancer and to determine its relationship with well-defined clinicopathological factors in this type of tumor. The study was conducted on 134 cases of invasive ductal breast carcinoma (IDC), 42 samples of non-malignant breast tissue (NMBT), and 26 cases of mastopathy. Moreover, selected breast cancer cell lines (MCF-7, SKBR-3, MDA-MB-231, BO2) and normal human breast epithelial cells (hTERT-HME1) were used. The expression of MT-3 was examined on the protein level using immunohistochemistry and on the mRNA level using real-time PCR. It was shown that the MT-3 protein in cells of IDC and mastopathy appeared in the cytoplasm as well as in the cell nuclei. Both the cytoplasmic and nuclear expression of MT-3 was significantly lower in IDC than in the mastopathies (p<0.0001 and p<0.001). However, no significant correlation was demonstrated between the level of MT-3 protein and the studied clinicopathological factors. The mRNA expression of MT-3 in IDC was also lower than in non‑malignant breast tissue (p<0.0001). Furthermore, in the cases of IDC with lymph node metastasis, the level of MT-3 mRNA was significantly lower than in the cases without metastasis (p=0.0199). The expression of MT-3 mRNA in breast cancer cell lines was significantly lower than in the normal human breast epithelial cell line (p<0.001). These results suggest that MT-3 may play a role in the malignant transformation of breast epithelial cells and in tumor progression.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

View References

1 

Coyle P, Philcox JC, Carey LC and Rofe AM: Metallothionein: the multipurpose protein. Cell Mol Life Sci. 59:627–647. 2002. View Article : Google Scholar : PubMed/NCBI

2 

Romero-Isart N and Vasák M: Advances in the structure and chemistry of metallothioneins. J Inorg Biochem. 88:388–396. 2002. View Article : Google Scholar : PubMed/NCBI

3 

Dziegiel P, Pula B, Kobierzycki C, Stasiolek M and Podhorska-Okolow M: Metallothioneins: structure and functions. Adv Anat Embryol Cell Biol. 218:3–20. 2016. View Article : Google Scholar

4 

Vasák M: Advances in metallothionein structure and functions. J Trace Elem Med Biol. 19:13–17. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Thirumoorthy N, Manisenthil Kumar KT, Shyam Sundar A, Panayappan L and Chatterjee M: Metallothionein: an overview. World J Gastroenterol. 13:993–996. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Uchida Y, Takio K, Titani K, Ihara Y and Tomonaga M: The growth inhibitory factor that is deficient in the Alzheimer’s disease brain is a 68 amino acid metallothionein-like protein. Neuron. 7:337–347. 1991. View Article : Google Scholar : PubMed/NCBI

7 

Quaife CJ, Findley SD, Erickson JC, Froelick GJ, Kelly EJ, Zambrowicz BP and Palmiter RD: Induction of a new metallothionein isoform (MT-IV) occurs during differentiation of stratified squamous epithelia. Biochemistry. 33:7250–7259. 1994. View Article : Google Scholar : PubMed/NCBI

8 

Moffatt P and Séguin C: Expression of the gene encoding metallothionein-3 in organs of the reproductive system. DNA Cell Biol. 17:501–510. 1998. View Article : Google Scholar : PubMed/NCBI

9 

Haq F, Mahoney M and Koropatnick J: Signaling events for metallothionein induction. Mutat Res. 533:211–226. 2003. View Article : Google Scholar : PubMed/NCBI

10 

Hozumi I, Suzuki JS, Kanazawa H, Hara A, Saio M, Inuzuka T, Miyairi S, Naganuma A and Tohyama C: Metallothionein-3 is expressed in the brain and various peripheral organs of the rat. Neurosci Lett. 438:54–58. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Sato M and Kondoh M: Recent studies on metallothionein: protection against toxicity of heavy metals and oxygen free radicals. Tohoku J Exp Med. 196:9–22. 2002. View Article : Google Scholar : PubMed/NCBI

12 

Theocharis SE, Margeli AP and Koutselinis A: Metallothionein: a multifunctional protein from toxicity to cancer. Int J Biol Markers. 18:162–169. 2003.PubMed/NCBI

13 

Cherian MG, Jayasurya A and Bay BH: Metallothioneins in human tumors and potential roles in carcinogenesis. Mutat Res. 533:201–209. 2003. View Article : Google Scholar : PubMed/NCBI

14 

Nielsen AE, Bohr A and Penkowa M: The Balance between life and death of cells: roles of metallothioneins. Biomark Insights. 7:99–111. 2007.

15 

Theocharis SE, Margeli AP, Klijanienko JT and Kouraklis GP: Metallothionein expression in human neoplasia. Histopathology. 45:103–118. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Pedersen MØ, Larsen A, Stoltenberg M and Penkowa M: The role of metallothionein in oncogenesis and cancer prognosis. Prog Histochem Cytochem. 44:29–64. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Dziegiel P, Forgacz J, Suder E, Surowiak P, Kornafel J and Zabel M: Prognostic significance of metallothionein expression in correlation with Ki-67 expression in adenocarcinomas of large intestine. Histol Histopathol. 18:401–407. 2003.PubMed/NCBI

18 

Tai SK, Tan OJ, Chow VT, Jin R, Jones JL, Tan PH, Jayasurya A and Bay BH: Differential expression of metallothionein 1 and 2 isoforms in breast cancer lines with different invasive potential: identification of a novel nonsilent metallothionein-1H mutant variant. Am J Pathol. 163:2009–2019. 2003. View Article : Google Scholar : PubMed/NCBI

19 

Dziegiel P, Salwa-Zurawska W, Zurawski J, Wojnar A and Zabel M: Prognostic significance of augmented metallothionein (MT) expression correlated with Ki-67 antigen expression in selected soft tissue sarcomas. Histol Histopathol. 20:83–89. 2005.

20 

Gallicchio LM, Flaws JA, Fowler BA and Ioffe OB: Metallothionein expression in invasive and in situ breast carcinomas. Cancer Detect Prev. 29:332–337. 2005. View Article : Google Scholar : PubMed/NCBI

21 

Szajerka A, Dziegiel P, Szajerka T, Zabel M, Winowski J and Grzebieniak Z: Immunohistochemical evaluation of metallothionein, MCM-2 and Ki-67 antigen expression in tumors of the adrenal cortex. Anticancer Res. 28:2959–2965. 2008.PubMed/NCBI

22 

Cardoso SV, Silveira-Júnior JB, De Carvalho Machado V, De-Paula AM, Loyola AM and De Aguiar MC: Expression of metallothionein and p53 antigens are correlated in oral squamous cell carcinoma. Anticancer Res. 29:1189–1193. 2009.PubMed/NCBI

23 

Wojnar A, Pula B, Piotrowska A, Jethon A, Kujawa K, Kobierzycki C, Rys J, Podhorska-Okolow M and Dziegiel P: Correlation of intensity of MT-I/II expression with Ki-67 and MCM-2 proteins in invasive ductal breast carcinoma. Anticancer Res. 31:3027–3033. 2011.PubMed/NCBI

24 

Wang N, Dong CR, Jiang R, Tang C, Yang L, Jiang QF, Chen GG and Liu ZM: Overexpression of HIF-1α, metallothionein and SLUG is associated with high TNM stage and lymph node metastasis in papillary thyroid carcinoma. Int J Clin Exp Pathol. 15:322–330. 2013.

25 

Surowiak P, Matkowski R, Materna V, Györffy B, Wojnar A, Pudelko M, Dziegiel P, Kornafel J and Zabel M: Elevated metallothionein (MT) expression in invasive ductal breast cancers predicts tamoxifen resistance. Histol Histopathol. 20:1037–1044. 2005.PubMed/NCBI

26 

Surowiak P, Materna V, Maciejczyk A, Pudeko M, Markwitz E, Spaczyski M, Dietel M, Zabel M and Lage H: Nuclear metallothionein expression correlates with cisplatin resistance of ovarian cancer cells and poor clinical outcome. Virchows Arch. 450:279–285. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Yap X, Tan HY, Huang J, Lai Y, Yip GW, Tan PH and Bay BH: Over-expression of metallothionein predicts chemoresistance in breast cancer. J Pathol. 217:563–570. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Dziegiel P, Pula B, Kobierzycki C, Stasiolek M and Podhorska-Okolow M: The role of metallothioneins in carcinogenesis. Adv Anat Embryol Cell Biol. 218:29–63. 2016. View Article : Google Scholar

29 

Palmiter RD, Findley SD, Whitmore TE and Durnam DM: MT-III, a brain-specific member of the metallothionein gene family. Proc Natl Acad Sci USA. 89:6333–6337. 1992. View Article : Google Scholar : PubMed/NCBI

30 

Tsuji S, Kobayashi H, Uchida Y, Ihara Y and Miyataka T: Molecular cloning of human growth inhibitory factor cDNA and its downregulationin Alzheimer’s disease. EMBO J. 11:4843–4850. 1992.PubMed/NCBI

31 

Sogawa CA, Asanuma M, Sogawa N, Miyazaki I, Nakanishi T, Furuta H and Ogawa N: Localization, regulation, and function of metallothionein-III/growth inhibitory factor in the brain. Acta Med Okayama. 55:1–9. 2001.PubMed/NCBI

32 

Dziegiel P, Pula B, Kobierzycki C, Stasiolek M and Podhorska-Okolow M: Metallothionein-3. Adv Anat Embryol Cell Biol. 218:21–27. 2016. View Article : Google Scholar

33 

Sewell AK, Jensen LT, Erickson JC, Palmiter RD and Winge DR: Bioactivity of metallothionein-3 correlates with its novel beta domain sequence rather than metal binding properties. Biochemistry. 34:4740–4747. 1995. View Article : Google Scholar : PubMed/NCBI

34 

Hoey JG, Garrett SH, Sens MA, Todd JH and Sens DA: Expression of MT-3 mRNA in human kidney, proximal tubule cell cultures, and renal cell carcinoma. Toxicol Lett. 92:149–160. 1997. View Article : Google Scholar : PubMed/NCBI

35 

Garrett SH, Sens MA, Todd JH, Somji S and Sens DA: Expression of MT-3 protein in the human kidney. Toxicol Lett. 105:207–214. 1999. View Article : Google Scholar : PubMed/NCBI

36 

Garrett SH, Sens MA, Shukla D, Nestor S, Somji S, Todd JH and Sens DA: Metallothionein isoform 3 expression in the human prostate and cancer-derived cell lines. Prostate. 41:196–202. 1999. View Article : Google Scholar : PubMed/NCBI

37 

Kim D, Garrett SH, Sens MA, Somji S and Sens DA: Metallothionein isoform 3 and proximal tubule vectorial active transport. Kidney Int. 61:464–472. 2002. View Article : Google Scholar : PubMed/NCBI

38 

Irie Y, Mori F, Keung WM, Mizushima Y and Wakabayashi K: Expression of neuronal growth inhibitory factor (metallothionein-III) in the salivary gland. Physiol Res. 53:719–723. 2004.PubMed/NCBI

39 

Sens MA, Somji S, Lamm DL, Garrett SH, Slovinsky F, Todd JH and Sens DA: Metallothionein isoform 3 as a potential biomarker for human bladder cancer. Environ Health Perspect. 108:413–418. 2000. View Article : Google Scholar : PubMed/NCBI

40 

Sens MA, Somji S, Garrett SH, Beall CL and Sens DA: Metallothionein isoform 3 overexpression is associated with breast cancers having a poor prognosis. Am J Pathol. 159:21–26. 2001. View Article : Google Scholar : PubMed/NCBI

41 

El-Rifai W, Frierson HF Jr, Harper JC, Powell SM and Knuutila S: Expression profiling of gastric adenocarcinoma using cDNA array. Int J Cancer. 92:832–838. 2001. View Article : Google Scholar : PubMed/NCBI

42 

Smith E, Drew PA, Tian ZQ, De Young NJ, Liu JF, Mayne GC, Ruszkiewicz AR, Watson DI and Jamieson GG: Metallothionien 3 expression is frequently down-regulated in oesophageal squamous cell carcinoma by DNA methylation. Mol Cancer. 13:422005. View Article : Google Scholar

43 

Werynska B, Pula B, Muszczynska-Bernhard B, Gomulkiewicz A, Jethon A, Podhorska-Okolow M, Jankowska R and Dziegiel P: Expression of metallothionein-III in patients with non-small cell lung cancer. Anticancer Res. 33:965–974. 2013.PubMed/NCBI

44 

Ellis IO, Schnitt SJ, Sastre-Garau X, Bussolati G, Tavassoli FA, Eusebi V, Peterse JL, Mukai K, Tabár L, Jacquemier J, et al: Invasive breast carcinoma. Pathology and Genetics of Tumours of the Breast and Female Genital Organs. World Health Organization Classification of Tumours. Tavassoli FA and Devilee P: IARC Press; Lyon: pp. 13–59. 2003

45 

Remmele W and Stegner HE: Recommendation for uniform definition of an immunoreactive score (IRS) for immunohistochemical estrogen receptor detection (ER-ICA) in breast cancer. Pathologe. 8:138–140. 1987.(In German). PubMed/NCBI

46 

Gomulkiewicz A, Podhorska-Okolow M, Szulc R, Smorag Z, Wojnar A, Zabel M and Dziegiel P: Correlation between metallothionein (MT) expression and selected prognostic factors in ductal breast cancers. Folia Histochem Cytobiol. 48:242–248. 2010. View Article : Google Scholar : PubMed/NCBI

47 

Mueller-Holzner E, Fink V, Frede T and Marth C: Immunohistochemical determination of HER2 expression in breast cancer from core biopsy specimens: a reliable predictor of HER2 status of the whole tumor. Breast Cancer Res Treat. 69:13–19. 2001. View Article : Google Scholar

48 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCt method. Methods. 25:402–408. 2001. View Article : Google Scholar

49 

Amoureux MC, Wurch T and Pauwels PJ: Modulation of metallothionein-III mRNA content and growth rate of rat C6-glial cells by transfection with human 5-HT1D receptor genes. Biochem Biophys Res Commun. 214:639–645. 1995. View Article : Google Scholar : PubMed/NCBI

50 

You HJ, Oh DH, Choi CY, Lee DG, Hahm KS, Moon AR and Jeong HG: Protective effect of metallothionein-III on DNA damage in response to reactive oxygen species. Biochim Biophys Acta. 1573:33–38. 2002. View Article : Google Scholar : PubMed/NCBI

51 

Jeong HG, Youn CK, Cho HJ, Kim SH, Kim MH, Kim HB, Chang IY, Lee YS, Chung MH and You HJ: Metallothionein-III prevents gamma-ray-induced 8-oxoguanine accumulation in normal and hOGG1-depleted cells. J Biol Chem. 279:34138–34149. 2004. View Article : Google Scholar : PubMed/NCBI

52 

Hozumi I, Uchida Y, Watabe K, Sakamoto T and Inuzuka T: Growth inhibitory factor (GIF) can protect from brain damage due to stab wounds in rat brain. Neurosci Lett. 395:220–223. 2006. View Article : Google Scholar

53 

Kim HG, Hwang YP and Jeong HG: Metallothionein-III induces HIF-1 alpha-mediated VEGF expression in brain endothelial cells. Biochem Biophys Res Commun. 369:666–671. 2008. View Article : Google Scholar : PubMed/NCBI

54 

Luo Y, Xu Y, Bao Q, Ding Z, Zhu C, Huang ZX and Tan X: The molecular mechanism for human metallothionein-3 to protect against the neuronal cytotoxicity of Aβ(1–42) with Cu ions. J Biol Inorg Chem. 18:39–47. 2013. View Article : Google Scholar

55 

Somji S, Garrett SH, Zhou XD, Zheng Y, Sens DA and Sens MA: Absence of metallothionein 3 expression in breast cancer is a rare, but favorable marker of outcome that is under epigenetic control. Toxicol Environ Chem. 92:1673–1695. 2010. View Article : Google Scholar : PubMed/NCBI

56 

Cherian MG and Apostolova MD: Nuclear localization of metallothionein during cell proliferation and differentiation. Cell Mol Biol (Noisy-le-grand). 46:347–356. 2000.

57 

Apostolova MD, Chen S, Chakrabarti S and Cherian MG: High-glucose-induced metallothionein expression in endothelial cells: an endothelin-mediated mechanism. Am J Physiol Cell Physiol. 281:C899–C907. 2001.PubMed/NCBI

58 

Bay BH, Jin R and Jayasurya A: Analysis of metallothionein expression in human cancers. Acta Histochem Cytochem. 34:171–176. 2001. View Article : Google Scholar

59 

Takahashi Y, Ogra Y and Suzuki KT: Nuclear trafficking of metallothionein requires oxidation of a cytosolic partner. J Cell Physiol. 202:563–569. 2005. View Article : Google Scholar

60 

Szelachowska J, Dziegiel P, Jelen-Krzeszewska J, Jelen M, Tarkowski R, Wlodarska I, Spytkowska B, Gisterek I, Matkowski R and Kornafel J: Prognostic significance of nuclear and cytoplasmic expression of metallothioneins as related to proliferative activity in squamous cell carcinomas of oral cavity. Histol Histopathol. 23:843–851. 2008.PubMed/NCBI

61 

Kobierzycki C, Pula B, Skiba M, Jablonska K, Latkowski K, Zabel M, Nowak-Markwitz E, Spaczynski M, Kedzia W, Podhorska-Okolow M, et al: Comparison of minichromosome maintenance proteins (MCM-3, MCM-7) and metallothioneins (MT-I/II, MT-III) expression in relation to clinicopathological data in ovarian cancer. Anticancer Res. 33:5375–5383. 2013.PubMed/NCBI

62 

Elias AD: Triple-negative breast cancer: a short review. Am J Clin Oncol. 33:637–645. 2010. View Article : Google Scholar

63 

Lin NU, Vanderplas A, Hughes ME, Theriault RL, Edge SB, Wong YN, Blayney DW, Niland JC, Winer EP and Weeks JC: Clinicopathologic features, patterns of recurrence, and survival among women with triple-negative breast cancer in the National Comprehensive Cancer Network. Cancer. 118:5463–5472. 2012. View Article : Google Scholar : PubMed/NCBI

64 

Kmiecik AM, Pula B, Suchanski J, Olbromski M, Gomulkiewicz A, Owczarek T, Kruczak A, Ambicka A, Rys J, Ugorski, et al: Metallothionein-3 increases triple-negative breast cancer cell invasiveness via induction of metalloproteinase expression. PLoS One. 10:e01248652015. View Article : Google Scholar : PubMed/NCBI

65 

Deng D, El-Rifai W, Ji J, Zhu B, Trampont P, Li J, Smith MF and Powel SM: Hypermethylation of metallothionein-3 CpG island in gastric carcinoma. Carcinogenesis. 24:25–29. 2003. View Article : Google Scholar : PubMed/NCBI

66 

Peng D, Hu TL, Jiang A, Washington MK, Moskaluk CA, Schneider-Stock R and El-Rifai W: Location-specific epigenetic regulation of the metallothionein 3 gene in esophageal adenocarcinomas. PLoS One. 6:e220092011. View Article : Google Scholar : PubMed/NCBI

67 

Tao YF, Xu LX, Lu J, Cao L, Li ZH, Hu SY, Wang NN, Du XJ, Sun LC, Zhao WL, et al: Metallothionein III (MT3) is a putative tumor suppressor gene that is frequently inactivated in pediatric acute myeloid leukemia by promoter hypermethylation. J Transl Med. 12:1822014. View Article : Google Scholar : PubMed/NCBI

68 

Dutta R, Sens DA, Somji S, Sens MA and Garrett SH: Metallothionein isoform 3 expression inhibits cell growth and increases drug resistance of PC-3 prostate cancer cells. Prostate. 52:89–97. 2002. View Article : Google Scholar : PubMed/NCBI

69 

Gurel V, Sens DA, Somji S, Garrett SH, Nath J and Sens MA: Stable transfection and overexpression of metallothionein isoform 3 inhibits the growth of MCF-7 and Hs578T cells but not that of T-47D or MDA-MB-231 cells. Breast Cancer Res Treat. 80:181–191. 2003. View Article : Google Scholar : PubMed/NCBI

70 

Tian ZQ, Xu YZ, Zhang YF, Ma GF, He M and Wang GY: Effects of metallothionein-3 and metallothionein-1E gene transfection on proliferation, cell cycle, and apoptosis of esophageal cancer cells. Genet Mol Res. 12:4595–4603. 2013. View Article : Google Scholar : PubMed/NCBI

71 

Ramaswamy S, Ross KN, Lander ES and Golub TR: A molecular signature of metastasis in primary solid tumors. Nat Genet. 33:49–54. 2003. View Article : Google Scholar

72 

Giorgi RR, Correa-Giannella ML, Casarini AP, Machado MC, Bronstein MD, Cescato VA and Giannella-Neto D: Metallothionein isoform 3 gene is differentially expressed in corticotropin-producing pituitary adenomas. Neuroendocrinology. 82:208–214. 2005. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Gomulkiewicz A, Jablonska K, Pula B, Grzegrzolka J, Borska S, Podhorska-Okolow M, Wojnar A, Rys J, Ambicka A, Ugorski M, Ugorski M, et al: Expression of metallothionein 3 in ductal breast cancer. Int J Oncol 49: 2487-2497, 2016.
APA
Gomulkiewicz, A., Jablonska, K., Pula, B., Grzegrzolka, J., Borska, S., Podhorska-Okolow, M. ... Dziegiel, P. (2016). Expression of metallothionein 3 in ductal breast cancer. International Journal of Oncology, 49, 2487-2497. https://doi.org/10.3892/ijo.2016.3759
MLA
Gomulkiewicz, A., Jablonska, K., Pula, B., Grzegrzolka, J., Borska, S., Podhorska-Okolow, M., Wojnar, A., Rys, J., Ambicka, A., Ugorski, M., Zabel, M., Dziegiel, P."Expression of metallothionein 3 in ductal breast cancer". International Journal of Oncology 49.6 (2016): 2487-2497.
Chicago
Gomulkiewicz, A., Jablonska, K., Pula, B., Grzegrzolka, J., Borska, S., Podhorska-Okolow, M., Wojnar, A., Rys, J., Ambicka, A., Ugorski, M., Zabel, M., Dziegiel, P."Expression of metallothionein 3 in ductal breast cancer". International Journal of Oncology 49, no. 6 (2016): 2487-2497. https://doi.org/10.3892/ijo.2016.3759
Copy and paste a formatted citation
x
Spandidos Publications style
Gomulkiewicz A, Jablonska K, Pula B, Grzegrzolka J, Borska S, Podhorska-Okolow M, Wojnar A, Rys J, Ambicka A, Ugorski M, Ugorski M, et al: Expression of metallothionein 3 in ductal breast cancer. Int J Oncol 49: 2487-2497, 2016.
APA
Gomulkiewicz, A., Jablonska, K., Pula, B., Grzegrzolka, J., Borska, S., Podhorska-Okolow, M. ... Dziegiel, P. (2016). Expression of metallothionein 3 in ductal breast cancer. International Journal of Oncology, 49, 2487-2497. https://doi.org/10.3892/ijo.2016.3759
MLA
Gomulkiewicz, A., Jablonska, K., Pula, B., Grzegrzolka, J., Borska, S., Podhorska-Okolow, M., Wojnar, A., Rys, J., Ambicka, A., Ugorski, M., Zabel, M., Dziegiel, P."Expression of metallothionein 3 in ductal breast cancer". International Journal of Oncology 49.6 (2016): 2487-2497.
Chicago
Gomulkiewicz, A., Jablonska, K., Pula, B., Grzegrzolka, J., Borska, S., Podhorska-Okolow, M., Wojnar, A., Rys, J., Ambicka, A., Ugorski, M., Zabel, M., Dziegiel, P."Expression of metallothionein 3 in ductal breast cancer". International Journal of Oncology 49, no. 6 (2016): 2487-2497. https://doi.org/10.3892/ijo.2016.3759
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team