miR-93 inhibits the invasive potential of triple-negative breast cancer cells in vitro via protein kinase WNK1

  • Authors:
    • Sukanya Shyamasundar
    • Jia Pei Lim
    • Boon Huat Bay
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  • Published online on: November 7, 2016     https://doi.org/10.3892/ijo.2016.3761
  • Pages: 2629-2636
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Abstract

Despite advances in treatment, the highly metastatic nature of breast tumors has given rise to the urgent need for development of novel therapeutic and prognostic markers. miR-93 is known to regulate the epithelial to mesenchymal transition process and to influence metastatic spread in breast carcinoma, although the exact mechanism(s)/genes involved remain unknown. In the present study, we examined the role of miR-93 in MDA-MB-231 breast cancer cells. Overexpression of mature miR-93-5p in MDA-MB-231 cells decreased cell migratory capability and invasive potential, as well as increased adhesion. In contrast, inhibition of miR-93 induced the opposite effects. miRNA-mRNA target prediction (TargetScan) identified WNK lysine deficient protein kinase 1 (WNK1), which is known to interact with diverse signaling pathways and regulate cell proliferation, survival, angiogenesis and metastasis, as one of the potential targets of miR-93. Furthermore, we showed by luciferase assay that WNK1 is a putative miR-93 target. siRNA mediated silencing of WNK1 also decreased the invasive ability of the cells, suggesting that the effects of miR-93 may be attributed at least in part to decreased WNK1 expression. Further in vivo studies are required to ascertain the miR-93-WNK1-metastasis cascade, that has potential implications in breast cancer therapy.
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December-2016
Volume 49 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Shyamasundar S, Lim JP and Bay BH: miR-93 inhibits the invasive potential of triple-negative breast cancer cells in vitro via protein kinase WNK1. Int J Oncol 49: 2629-2636, 2016.
APA
Shyamasundar, S., Lim, J.P., & Bay, B.H. (2016). miR-93 inhibits the invasive potential of triple-negative breast cancer cells in vitro via protein kinase WNK1. International Journal of Oncology, 49, 2629-2636. https://doi.org/10.3892/ijo.2016.3761
MLA
Shyamasundar, S., Lim, J. P., Bay, B. H."miR-93 inhibits the invasive potential of triple-negative breast cancer cells in vitro via protein kinase WNK1". International Journal of Oncology 49.6 (2016): 2629-2636.
Chicago
Shyamasundar, S., Lim, J. P., Bay, B. H."miR-93 inhibits the invasive potential of triple-negative breast cancer cells in vitro via protein kinase WNK1". International Journal of Oncology 49, no. 6 (2016): 2629-2636. https://doi.org/10.3892/ijo.2016.3761