HOXC6 regulates the antitumor effects of pheophorbide a-based photodynamic therapy in multidrug-resistant oral cancer cells

  • Authors:
    • Soo-A Kim
    • Mi Rha Lee
    • Jung-Hoon Yoon
    • Sang-Gun Ahn
  • View Affiliations

  • Published online on: November 10, 2016     https://doi.org/10.3892/ijo.2016.3766
  • Pages: 2421-2430
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Abstract

Photodynamic therapy (PDT) has been considered to be a possible candidate approach for the treatment of multidrug-resistant (MDR) cancer. To investigate the photocytotoxicity of pheophorbide a-based PDT on MDR cells, the intracellular pathways were studied using the human oral cancer FaDu cell line and its paclitaxel-selected subline FaDu-PTX. Pheophorbide a (Pa)-PDT induced significant photocytotoxicity in both FaDu and FaDu-PTX cell lines with cell apoptosis greater in FaDu cells compared to FaDu-PTX cells. We found that Hoxc6 and MDR-1 expression was strongly detected in FaDu-PTX cells compared to FaDu cells. Intriguingly, Pa-PDT effectively reduced Hoxc6 and MDR-1 expression in FaDu-PTX cells. The siRNA for HOXC6 can inhibit the intracellular MDR-1 levels in FaDu-PTX cells and induce the phototoxic effects of Pa-PDT. Furthermore, our in vivo studies showed that the Pa-PDT and HOXC6 siRNA significantly reduce the growth of FaDu-PTX xenograft tumors in C3H mice compared with control- and PTX-treated tumors. Histopathology was also used to confirm this antitumor effect. Pa-PDT may be a potential therapeutic modality for multidrug-resistant cancer, and Hoxc6, as a possible contributor to MDR, may reduce the therapeutic potential in multidrug-resistant oral malignancies.
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December-2016
Volume 49 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Kim S, Lee MR, Yoon J and Ahn S: HOXC6 regulates the antitumor effects of pheophorbide a-based photodynamic therapy in multidrug-resistant oral cancer cells. Int J Oncol 49: 2421-2430, 2016.
APA
Kim, S., Lee, M.R., Yoon, J., & Ahn, S. (2016). HOXC6 regulates the antitumor effects of pheophorbide a-based photodynamic therapy in multidrug-resistant oral cancer cells. International Journal of Oncology, 49, 2421-2430. https://doi.org/10.3892/ijo.2016.3766
MLA
Kim, S., Lee, M. R., Yoon, J., Ahn, S."HOXC6 regulates the antitumor effects of pheophorbide a-based photodynamic therapy in multidrug-resistant oral cancer cells". International Journal of Oncology 49.6 (2016): 2421-2430.
Chicago
Kim, S., Lee, M. R., Yoon, J., Ahn, S."HOXC6 regulates the antitumor effects of pheophorbide a-based photodynamic therapy in multidrug-resistant oral cancer cells". International Journal of Oncology 49, no. 6 (2016): 2421-2430. https://doi.org/10.3892/ijo.2016.3766