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Article

Downregulation of ST6GALNAC1 is associated with esophageal squamous cell carcinoma development

  • Authors:
    • Takeshi Iwaya
    • Genta Sawada
    • Suburu Amano
    • Kohei Kume
    • Chie Ito
    • Fumitaka Endo
    • Masafumi Konosu
    • Yoshihiro Shioi
    • Yuji Akiyama
    • Takeshi Takahara
    • Koki Otsuka
    • Hiroyuki Nitta
    • Keisuke Koeda
    • Masaru Mizuno
    • Satoshi Nishizuka
    • Akira Sasaki
    • Koshi Mimori
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, Iwate Medical University, Morioka, Iwate 020-8505, Japan, Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka, University, Suita 565-0871, Japan, Department of Surgery, Kyushu University Beppu Hospital, Beppu 874-0838, Japan
  • Pages: 441-447
    |
    Published online on: December 22, 2016
       https://doi.org/10.3892/ijo.2016.3817
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Abstract

Tylosis is an inherited disorder characterized by abnormal palmoplantar skin thickening and a highly elevated risk of esophageal squamous cell carcinoma (ESCC). Analyses of tylosis in families have localized the responsible gene locus to a region of chromosome 17q25.1. Frequent loss of heterozygosity (LOH) in 17q25.1 was also observed in the sporadic form of ESCC. A putative tumor suppressor gene for ESCC may exist at this locus. We investigated the expression patterns of genes on 17q25.1 in tumor and corresponding normal tissues from patients with sporadic ESCC using RNA sequence analysis. For candidate genes, quantitative real-time reverse transcription-PCR (qRT-PCR), direct sequence, LOH and methylation analyses were performed using 93 clinical ESCC samples and 10 cell lines. A significant downregulation of ST6GALNAC1 was demonstrated in ESCC tissues compared to its expression in normal tissues by qRT-PCR (n=93, p<0.0001). Frequent LOH (17/27, 62.9%) and hyper‑methylation in ST6GALNAC1 were also observed in all cell lines. Our results indicated that ST6GALNAC1 was downregulated in sporadic ESCC via hyper-methylation and LOH, and it may be a candidate responsible gene for ESCC. Furthermore, recent studies suggest that multiple genes on chromosome 17q25 are involved in ESCC development.
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Copy and paste a formatted citation
Spandidos Publications style
Iwaya T, Sawada G, Amano S, Kume K, Ito C, Endo F, Konosu M, Shioi Y, Akiyama Y, Takahara T, Takahara T, et al: Downregulation of ST6GALNAC1 is associated with esophageal squamous cell carcinoma development. Int J Oncol 50: 441-447, 2017.
APA
Iwaya, T., Sawada, G., Amano, S., Kume, K., Ito, C., Endo, F. ... Mimori, K. (2017). Downregulation of ST6GALNAC1 is associated with esophageal squamous cell carcinoma development. International Journal of Oncology, 50, 441-447. https://doi.org/10.3892/ijo.2016.3817
MLA
Iwaya, T., Sawada, G., Amano, S., Kume, K., Ito, C., Endo, F., Konosu, M., Shioi, Y., Akiyama, Y., Takahara, T., Otsuka, K., Nitta, H., Koeda, K., Mizuno, M., Nishizuka, S., Sasaki, A., Mimori, K."Downregulation of ST6GALNAC1 is associated with esophageal squamous cell carcinoma development". International Journal of Oncology 50.2 (2017): 441-447.
Chicago
Iwaya, T., Sawada, G., Amano, S., Kume, K., Ito, C., Endo, F., Konosu, M., Shioi, Y., Akiyama, Y., Takahara, T., Otsuka, K., Nitta, H., Koeda, K., Mizuno, M., Nishizuka, S., Sasaki, A., Mimori, K."Downregulation of ST6GALNAC1 is associated with esophageal squamous cell carcinoma development". International Journal of Oncology 50, no. 2 (2017): 441-447. https://doi.org/10.3892/ijo.2016.3817
Copy and paste a formatted citation
x
Spandidos Publications style
Iwaya T, Sawada G, Amano S, Kume K, Ito C, Endo F, Konosu M, Shioi Y, Akiyama Y, Takahara T, Takahara T, et al: Downregulation of ST6GALNAC1 is associated with esophageal squamous cell carcinoma development. Int J Oncol 50: 441-447, 2017.
APA
Iwaya, T., Sawada, G., Amano, S., Kume, K., Ito, C., Endo, F. ... Mimori, K. (2017). Downregulation of ST6GALNAC1 is associated with esophageal squamous cell carcinoma development. International Journal of Oncology, 50, 441-447. https://doi.org/10.3892/ijo.2016.3817
MLA
Iwaya, T., Sawada, G., Amano, S., Kume, K., Ito, C., Endo, F., Konosu, M., Shioi, Y., Akiyama, Y., Takahara, T., Otsuka, K., Nitta, H., Koeda, K., Mizuno, M., Nishizuka, S., Sasaki, A., Mimori, K."Downregulation of ST6GALNAC1 is associated with esophageal squamous cell carcinoma development". International Journal of Oncology 50.2 (2017): 441-447.
Chicago
Iwaya, T., Sawada, G., Amano, S., Kume, K., Ito, C., Endo, F., Konosu, M., Shioi, Y., Akiyama, Y., Takahara, T., Otsuka, K., Nitta, H., Koeda, K., Mizuno, M., Nishizuka, S., Sasaki, A., Mimori, K."Downregulation of ST6GALNAC1 is associated with esophageal squamous cell carcinoma development". International Journal of Oncology 50, no. 2 (2017): 441-447. https://doi.org/10.3892/ijo.2016.3817
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