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High Rab11-FIP4 expression predicts poor prognosis and exhibits tumor promotion in pancreatic cancer

  • Authors:
    • Yun He
    • Mengsi Ye
    • Lingling Zhou
    • Yunfeng Shan
    • Guangrong Lu
    • Yuhui Zhou
    • Jinwei Zhong
    • Jihang Zheng
    • Zhanxiong Xue
    • Zhenzhai Cai
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China, Department of Pathology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China, Department of Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China, Department of General Surgery, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China
    Copyright: © He et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 396-404
    |
    Published online on: December 30, 2016
       https://doi.org/10.3892/ijo.2016.3828
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Abstract

Some studies have demonstrated that Rab11-family interacting proteins (Rab11-FIPs) are connected with the tumorigenesis, and they may act as tumor promoters in some cancers. The clinicopathological significance of Rab11-family interacting protein 4 (Rab11-FIP4 ) expression and its possible effects on pancreatic cancer (PC) are still undiscovered. In this study, Rab11-FIP4 protein expression level in 60 PC specimens and pair-matched non-cancerous samples were detected by immunohistochemistry analysis. The results were analysed and compared with each patients' clinical data. Rab11-FIP4 expression in PC tissues increased significantly more than that of adjacent non-cancerous tissues (P=0.0001). Overexpression of Rab11-FIP4 in the PC tissues was significantly related to tumor size (P=0.0001), histological grade (P=0.028), metastasis (P=0.001) and TNM stage (P=0.004) but not with age (P=0.832), gender (P=0.228) or tumor site (P=0.875). Kaplan-Meier survival analysis showed that overexpression of Rab11-FIP4 was significantly related to overall survival time (P=0.0036). In addition, Rab11-FIP4 in PANC-1 pancreatic cancer cells were successfully knocked-out using the CRISPR/Cas9 system. Rab11-FIP4 knockout in PANC-1 cells inhibited cell growth, invasion and metastasis, and arrested cell cycle progression, but did not alter apoptosis. Our findings suggest that overexpression of Rab11-FIP4 predicts poor clinical outcomes for pancreatic cancer and contributes to pancreatic tumor progression.
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1 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2016. CA Cancer J Clin. 66:7–30. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Goral V: Pancreatic cancer: Pathogenesis and diagnosis. Asian Pac J Cancer Prev. 16:5619–5624. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Matsuno S, Egawa S, Fukuyama S, Motoi F, Sunamura M, Isaji S, Imaizumi T, Okada S, Kato H, Suda K, et al: Pancreatic Cancer Registry in Japan: 20 years of experience. Pancreas. 28:219–230. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Ullrich O, Reinsch S, Urbé S, Zerial M and Parton RG: Rab11 regulates recycling through the pericentriolar recycling endosome. J Cell Biol. 135:913–924. 1996. View Article : Google Scholar : PubMed/NCBI

5 

Wilcke M, Johannes L, Galli T, Mayau V, Goud B and Salamero J: Rab11 regulates the compartmentalization of early endosomes required for efficient transport from early endosomes to the trans-golgi network. J Cell Biol. 151:1207–1220. 2000. View Article : Google Scholar : PubMed/NCBI

6 

Riggs B, Rothwell W, Mische S, Hickson GR, Matheson J, Hays TS, Gould GW and Sullivan W: Actin cytoskeleton remodeling during early Drosophila furrow formation requires recycling endosomal components Nuclear-fallout and Rab11. J Cell Biol. 163:143–154. 2003. View Article : Google Scholar : PubMed/NCBI

7 

Junutula JR, Schonteich E, Wilson GM, Peden AA, Scheller RH and Prekeris R: Molecular characterization of Rab11 interactions with members of the family of Rab11-interacting proteins. J Biol Chem. 279:33430–33437. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Horgan CP and McCaffrey MW: The dynamic Rab11-FIPs. Biochem Soc Trans. 37:1032–1036. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Hales CM, Griner R, Hobdy-Henderson KC, Dorn MC, Hardy D, Kumar R, Navarre J, Chan EK, Lapierre LA and Goldenring JR: Identification and characterization of a family of Rab11-interacting proteins. J Biol Chem. 276:39067–39075. 2001. View Article : Google Scholar : PubMed/NCBI

10 

Fielding AB, Schonteich E, Matheson J, Wilson G, Yu X, Hickson GR, Srivastava S, Baldwin SA, Prekeris R and Gould GW: Rab11-FIP3 and FIP4 interact with Arf6 and the exocyst to control membrane traffic in cytokinesis. EMBO J. 24:3389–3399. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Muto A, Aoki Y and Watanabe S: Mouse Rab11-FIP4 regulates proliferation and differentiation of retinal progenitors in a Rab11-independent manner. Dev Dyn. 236:214–225. 2007. View Article : Google Scholar

12 

Muto A, Arai K and Watanabe S: Rab11-FIP4 is predominantly expressed in neural tissues and involved in proliferation as well as in differentiation during zebrafish retinal development. Dev Biol. 292:90–102. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Yoon SO, Shin S and Mercurio AM: Hypoxia stimulates carcinoma invasion by stabilizing microtubules and promoting the Rab11 trafficking of the alpha6beta4 integrin. Cancer Res. 65:2761–2769. 2005. View Article : Google Scholar : PubMed/NCBI

14 

Hu F, Deng X, Yang X, Jin H, Gu D, Lv X, Wang C, Zhang Y, Huo X, Shen Q, et al: Hypoxia upregulates Rab11-family interacting protein 4 through HIF-1α to promote the metastasis of hepatocellular carcinoma. Oncogene. 34:6007–6017. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Chaika NV, Gebregiworgis T, Lewallen ME, Purohit V, Radhakrishnan P, Liu X, Zhang B, Mehla K, Brown RB, Caffrey T, et al: MUC1 mucin stabilizes and activates hypoxia-inducible factor 1 alpha to regulate metabolism in pancreatic cancer. Proc Natl Acad Sci USA. 109:13787–13792. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Ye LY, Zhang Q, Bai XL, Pankaj P, Hu QD and Liang TB: Hypoxia-inducible factor 1α expression and its clinical significance in pancreatic cancer: A meta-analysis. Pancreatology. 14:391–397. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Hao XP, Willis JE, Pretlow TG, Rao JS, MacLennan GT, Talbot IC and Pretlow TP: Loss of fragile histidine triad expression in colorectal carcinomas and premalignant lesions. Cancer Res. 60:18–21. 2000.PubMed/NCBI

18 

Xue Z, Zhou Y, Wang C, Zheng J, Zhang P, Zhou L, Wu L, Shan Y, Ye M, He Y, et al: Latexin exhibits tumor-suppressor potential in pancreatic ductal adenocarcinoma. Oncol Rep. 35:50–58. 2016.

19 

Cong L and Zhang F: Genome engineering using CRISPR-Cas9 system. Methods Mol Biol. 1239:197–217. 2015. View Article : Google Scholar

20 

Zhou Y, Zhu S, Cai C, Yuan P, Li C, Huang Y and Wei W: High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells. Nature. 509:487–491. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelsen TS, Heckl D, Ebert BL, Root DE, Doench JG, et al: Genome-scale CRISPR-Cas9 knockout screening in human cells. Science. 343:84–87. 2014. View Article : Google Scholar :

22 

Hsu PD, Lander ES and Zhang F: Development and applications of CRISPR-Cas9 for genome engineering. Cell. 157:1262–1278. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Rachagani S, Macha MA, Heimann N, Seshacharyulu P, Haridas D, Chugh S and Batra SK: Clinical implications of miRNAs in the pathogenesis, diagnosis and therapy of pancreatic cancer. Adv Drug Deliv Rev. 81:16–33. 2015. View Article : Google Scholar :

24 

Shahrokni A and Saif MW: Metastatic pancreatic cancer: The dilemma of quality vs. quantity of life. JOP. 14:391–394. 2013.PubMed/NCBI

25 

Horgan CP, Hanscom SR, Kelly EE and McCaffrey MW: Tumor susceptibility gene 101 (TSG101) is a novel binding-partner for the class II Rab11-FIPs. PLoS One. 7:e320302012. View Article : Google Scholar : PubMed/NCBI

26 

Cheng H, Sugiura R, Wu W, Fujita M, Lu Y, Sio SO, Kawai R, Takegawa K, Shuntoh H and Kuno T: Role of the Rab GTP-binding protein Ypt3 in the fission yeast exocytic pathway and its connection to calcineurin function. Mol Biol Cell. 13:2963–2976. 2002. View Article : Google Scholar : PubMed/NCBI

27 

Krzyzaniak MA, Mach M and Britt WJ: HCMV-encoded glycoprotein M (UL100) interacts with Rab11 effector protein FIP4. Traffic. 10:1439–1457. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Wallace DM, Lindsay AJ, Hendrick AG and McCaffrey MW: Rab11-FIP4 interacts with Rab11 in a GTP-dependent manner and its overexpression condenses the Rab11 positive compartment in HeLa cells. Biochem Biophys Res Commun. 299:770–779. 2002. View Article : Google Scholar : PubMed/NCBI

29 

Xu CL, Wang JZ, Xia XP, Pan CW, Shao XX, Xia SL, Yang SX and Zheng B: Rab11-FIP2 promotes colorectal cancer migration and invasion by regulating PI3K/AKT/MMP7 signaling pathway. Biochem Biophys Res Commun. 470:397–404. 2016. View Article : Google Scholar : PubMed/NCBI

30 

Zhang J, Liu X, Datta A, Govindarajan K, Tam WL, Han J, George J, Wong C, Ramnarayanan K, Phua TY, et al: RCP is a human breast cancer-promoting gene with Ras-activating function. J Clin Invest. 119:2171–2183. 2009.PubMed/NCBI

31 

Jing J, Tarbutton E, Wilson G and Prekeris R: Rab11-FIP3 is a Rab11-binding protein that regulates breast cancer cell motility by modulating the actin cytoskeleton. Eur J Cell Biol. 88:325–341. 2009. View Article : Google Scholar : PubMed/NCBI

32 

Dong W, Qin G and Shen R: Rab11-FIP2 promotes the metastasis of gastric cancer cells. Int J Cancer. 138:1680–1688. 2016. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
He Y, Ye M, Zhou L, Shan Y, Lu G, Zhou Y, Zhong J, Zheng J, Xue Z, Cai Z, Cai Z, et al: High Rab11-FIP4 expression predicts poor prognosis and exhibits tumor promotion in pancreatic cancer. Int J Oncol 50: 396-404, 2017.
APA
He, Y., Ye, M., Zhou, L., Shan, Y., Lu, G., Zhou, Y. ... Cai, Z. (2017). High Rab11-FIP4 expression predicts poor prognosis and exhibits tumor promotion in pancreatic cancer. International Journal of Oncology, 50, 396-404. https://doi.org/10.3892/ijo.2016.3828
MLA
He, Y., Ye, M., Zhou, L., Shan, Y., Lu, G., Zhou, Y., Zhong, J., Zheng, J., Xue, Z., Cai, Z."High Rab11-FIP4 expression predicts poor prognosis and exhibits tumor promotion in pancreatic cancer". International Journal of Oncology 50.2 (2017): 396-404.
Chicago
He, Y., Ye, M., Zhou, L., Shan, Y., Lu, G., Zhou, Y., Zhong, J., Zheng, J., Xue, Z., Cai, Z."High Rab11-FIP4 expression predicts poor prognosis and exhibits tumor promotion in pancreatic cancer". International Journal of Oncology 50, no. 2 (2017): 396-404. https://doi.org/10.3892/ijo.2016.3828
Copy and paste a formatted citation
x
Spandidos Publications style
He Y, Ye M, Zhou L, Shan Y, Lu G, Zhou Y, Zhong J, Zheng J, Xue Z, Cai Z, Cai Z, et al: High Rab11-FIP4 expression predicts poor prognosis and exhibits tumor promotion in pancreatic cancer. Int J Oncol 50: 396-404, 2017.
APA
He, Y., Ye, M., Zhou, L., Shan, Y., Lu, G., Zhou, Y. ... Cai, Z. (2017). High Rab11-FIP4 expression predicts poor prognosis and exhibits tumor promotion in pancreatic cancer. International Journal of Oncology, 50, 396-404. https://doi.org/10.3892/ijo.2016.3828
MLA
He, Y., Ye, M., Zhou, L., Shan, Y., Lu, G., Zhou, Y., Zhong, J., Zheng, J., Xue, Z., Cai, Z."High Rab11-FIP4 expression predicts poor prognosis and exhibits tumor promotion in pancreatic cancer". International Journal of Oncology 50.2 (2017): 396-404.
Chicago
He, Y., Ye, M., Zhou, L., Shan, Y., Lu, G., Zhou, Y., Zhong, J., Zheng, J., Xue, Z., Cai, Z."High Rab11-FIP4 expression predicts poor prognosis and exhibits tumor promotion in pancreatic cancer". International Journal of Oncology 50, no. 2 (2017): 396-404. https://doi.org/10.3892/ijo.2016.3828
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