Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Oncology
Join Editorial Board Propose a Special Issue
Print ISSN: 1019-6439 Online ISSN: 1791-2423
Journal Cover
February-2017 Volume 50 Issue 2

Full Size Image

Cover Legend PDF

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
February-2017 Volume 50 Issue 2

Full Size Image

Cover Legend PDF

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Identification of potential targets for differentiation in human leukemia cells induced by diallyl disulfide

  • Authors:
    • Hui Ling
    • Jie He
    • Hui Tan
    • Lan Yi
    • Fang Liu
    • Xiaoxia Ji
    • Youhua Wu
    • Haobin Hu
    • Xi Zeng
    • Xiaohong Ai
    • Hao Jiang
    • Qi Su
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Tumor Cellular and Molecular Pathology, College of Hunan Province, Cancer Research Institute, University of South China, Hengyang, Hunan 421001, P.R. China, The First Affiliated Hospital, University of South China, Hengyang, Hunan 421001, P.R. China
  • Pages: 697-707
    |
    Published online on: January 5, 2017
       https://doi.org/10.3892/ijo.2017.3839
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Diallyl disulfide (DADS) is a primary component of garlic, which has chemopreventive potential. We previously found that moderate doses (15-120 µM) of DADS induced apoptosis and G2/M phase cell cycle arrest. In this study, we observed the effect of low doses (8 µM) of DADS on human leukemia HL-60 cells. We found that DADS could inhibit proliferation, migration and invasion in HL-60 cells, and arrested cells at G0/G1 stage. Then, cell differentiation was displayed by morphologic observation, NBT reduction activity and CD11b evaluation of cytometric flow. It showed that DADS induced differentiation, reduced the ability of NBT and increased CD11b expression. Likewise, DADS inhibited xenograft tumor growth and induced differentiation in vivo. In order to make sure how DADS induced differentiation, we compared the protein expression profile of DADS-treated cells with that of untreated control. Using high resolution mass spectrometry, we identified 18 differentially expressed proteins after treatment with DADS, including four upregulated and 14 downregulated proteins. RT-PCR and western blot assay showed that DJ-1, cofilin 1, RhoGDP dissociation inhibitor 2 (RhoGDI2), Calreticulin (CTR) and PCNA were decreased by DADS. These data suggest that the effects of DADS on leukemia may be due to multiple targets for intervention.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Schlenk RF: Post-remission therapy for acute myeloid leukemia. Haematologica. 99:1663–1670. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Mrózek K, Marcucci G, Nicolet D, Maharry KS, Becker H, Whitman SP, Metzeler KH, Schwind S, Wu YZ, Kohlschmidt J, et al: Prognostic significance of the European LeukemiaNet standardized system for reporting cytogenetic and molecular alterations in adults with acute myeloid leukemia. J Clin Oncol. 30:4515–4523. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Morceau F, Chateauvieux S, Orsini M, Trécul A, Dicato M and Diederich M: Natural compounds and pharmaceuticals reprogram leukemia cell differentiation pathways. Biotechnol Adv. 33:785–797. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Sui M, Zhang Z and Zhou J: Inhibition factors of arsenic trioxide therapeutic effects in patients with acute promyelocytic leukemia. Chin Med J (Engl). 127:3503–3506. 2014.

5 

Gallagher RE: Retinoic acid resistance in acute promyelocytic leukemia. Leukemia. 16:1940–1958. 2002. View Article : Google Scholar : PubMed/NCBI

6 

Sanz MA and Montesinos P: How we prevent and treat differentiation syndrome in patients with acute promyelocytic leukemia. Blood. 123:2777–2782. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Kweon SH, Song JH, Kim HJ, Kim TS and Choi BG: Induction of human leukemia cell differentiation via PKC/MAPK pathways by arsantin, a sesquiterpene lactone from Artemisia santolina. Arch Pharm Res. 38:2020–2028. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Yi L and Su Q: Molecular mechanisms for the anticancer effects of diallyl disulfide. Food Chem Toxicol. 57:362–370. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Sundaram SG and Milner JA: Diallyl disulfide suppresses the growth of human colon tumor cell xenografts in athymic nude mice. J Nutr. 126:1355–1361. 1996.PubMed/NCBI

10 

Yi L, Ji XX, Lin M, Tan H, Tang Y, Wen L, Ma YH and Su Q: Diallyl disulfide induces apoptosis in human leukemia HL-60 cells through activation of JNK mediated by reactive oxygen. Pharmazie. 65:693–698. 2010.PubMed/NCBI

11 

Yi L, Ji XX, Tan H, Feng MY, Tang Y, Wen L and Su Q: Involvement of Mcl1 in diallyl disulfide-induced G2/M cell cycle arrest in HL-60 cells. Oncol Rep. 27:1911–1917. 2012.PubMed/NCBI

12 

Gharahdaghi F, Weinberg CR, Meagher DA, Imai BS and Mische SM: Mass spectrometric identification of proteins from silver-stained polyacrylamide gel: A method for the removal of silver ions to enhance sensitivity. Electrophoresis. 20:601–605. 1999. View Article : Google Scholar : PubMed/NCBI

13 

Ling H, Zhang LY, Su Q, Song Y, Luo ZY, Zhou XT, Zeng X, He J, Tan H and Yuan JP: Erk is involved in the differentiation induced by diallyl disulfide in the human gastric cancer cell line MGC803. Cell Mol Biol Lett. 11:408–423. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Lai KC, Hsu SC, Kuo CL, Yang JS, Ma CY, Lu HF, Tang NY, Hsia TC, Ho HC and Chung JG: Diallyl sulfide, diallyl disulfide, and diallyl trisulfide inhibit migration and invasion in human colon cancer colo 205 cells through the inhibition of matrix metalloproteinase-2, -7, and -9 expressions. Environ Toxicol. 28:479–488. 2013. View Article : Google Scholar

15 

Nagakubo D, Taira T, Kitaura H, Ikeda M, Tamai K, Iguchi-Ariga SM and Ariga H: DJ-1, a novel oncogene which transforms mouse NIH3T3 cells in cooperation with ras. Biochem Biophys Res Commun. 231:509–513. 1997. View Article : Google Scholar : PubMed/NCBI

16 

Zhou X, Xu N, Li R, Xiao Y, Gao G, Lu Q, Ding L, Li L, Li Y, Du Q, et al: A comparative proteomic study of Homoharringtonine-induced apoptosis in leukemia K562 cells. Leuk Lymphoma. 56:2162–2169. 2015. View Article : Google Scholar

17 

Liu H, Wang M, Li M, Wang D, Rao Q, Wang Y, Xu Z and Wang J: Expression and role of DJ-1 in leukemia. Biochem Biophys Res Commun. 375:477–483. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Bai J, Guo C, Sun W, Li M, Meng X, Yu Y, Jin Y, Tong D, Geng J, Huang Q, et al: DJ-1 may contribute to metastasis of non-small cell lung cancer. Mol Biol Rep. 39:2697–2703. 2012. View Article : Google Scholar

19 

Lee H, Choi SK and Ro JY: Overexpression of DJ-1 and HSP90α, and loss of PTEN associated with invasive urothelial carcinoma of urinary bladder: Possible prognostic markers. Oncol Lett. 3:507–512. 2012.PubMed/NCBI

20 

Kim YC, Kitaura H, Taira T, Iguchi-Ariga SM and Ariga H: Oxidation of DJ-1-dependent cell transformation through direct binding of DJ-1 to PTEN. Int J Oncol. 35:1331–1341. 2009.PubMed/NCBI

21 

Vasseur S, Afzal S, Tomasini R, Guillaumond F, Tardivel-Lacombe J, Mak TW and Iovanna JL: Consequences of DJ-1 upregulation following p53 loss and cell transformation. Oncogene. 31:664–670. 2012.

22 

Zhou J, Wang Y, Fei J and Zhang W: expression of cofilin 1 is positively correlated with the differentiation of human epithelial ovarian cancer. Oncol Lett. 4:1187–1190. 2012.PubMed/NCBI

23 

Harris W, Sachana M, Flaskos J and Hargreaves AJ: Proteomic analysis of differentiating neuroblastoma cells treated with sublethal neurite inhibitory concentrations of diazinon: Identification of novel biomarkers of effect. Toxicol Appl Pharmacol. 240:159–165. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Heasman SJ and Ridley AJ: Mammalian Rho GTPases: New insights into their functions from in vivo studies. Nat Rev Mol Cell Biol. 9:690–701. 2008. View Article : Google Scholar : PubMed/NCBI

25 

Koh SA, Kim MK, Lee KH, Kim SW and Kim JR: RhoGDI2 is associated with HGF-mediated tumor invasion through VEGF in stomach cancer. Clin exp Metastasis. 31:805–815. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Fang Y, Yi J, Lizhi L and Qiucheng C: Rho GDP dissociation inhibitor beta promotes cell proliferation and invasion by modulating the AKT pathway in hepatocellular carcinoma. DNA Cell Biol. 33:781–786. 2014. View Article : Google Scholar : PubMed/NCBI

27 

López-Pedrera C, Villalba JM, Siendones E, Barbarroja N, Gómez-Díaz C, Rodríguez-Ariza A, Buendía P, Torres A and Velasco F: Proteomic analysis of acute myeloid leukemia: Identification of potential early biomarkers and therapeutic targets. Proteomics. 6(Suppl 1): S293–S299. 2006. View Article : Google Scholar : PubMed/NCBI

28 

Griner EM and Theodorescu D: The faces and friends of RhoGDI2. Cancer Metastasis Rev. 31:519–528. 2012. View Article : Google Scholar : PubMed/NCBI

29 

Griner EM, Dancik GM, Costello JC, Owens C, Guin S, Edwards MG, Brautigan DL, Theodorescu D and Rho C: RhoC is an unexpected target of RhoGDI2 in prevention of lung colonization of bladder cancer. Mol Cancer Res. 13:483–492. 2015. View Article : Google Scholar :

30 

Zamanian M, Veerakumarasivam A, Abdullah S and Rosli R: Calreticulin and cancer. Pathol Oncol Res. 19:149–154. 2013. View Article : Google Scholar : PubMed/NCBI

31 

Weng WC, Lin KH, Wu PY, Lu YC, Weng YC, Wang BJ, Liao YF, Hsu WM, Lee WT and Lee H: Calreticulin regulates VEGF-A in neuroblastoma cells. Mol Neurobiol. 52:758–770. 2015. View Article : Google Scholar

32 

Lu YC, Weng WC and Lee H: Functional roles of calreticulin in cancer biology. Biomed Res Int. 2015:5265242015. View Article : Google Scholar : PubMed/NCBI

33 

Caputo E, Maiorana L, Vasta V, Pezzino FM, Sunkara S, Wynne K, Elia G, Marincola FM, McCubrey JA, Libra M, et al: Characterization of human melanoma cell lines and melanocytes by proteome analysis. Cell Cycle. 10:2924–2936. 2011. View Article : Google Scholar : PubMed/NCBI

34 

Qin H, Liu T, Yang JL, Huang X, Liu B, Song X, Zhao X and Wei YQ: Screening proteins related to retinoic acid resistance by proteomic analysis. Zhonghua Yi Xue Za Zhi. 87:520–525. 2007.In Chinese. PubMed/NCBI

35 

Wang SC: PCNA: A silent housekeeper or a potential therapeutic target? Trends Pharmacol Sci. 35:178–186. 2014. View Article : Google Scholar : PubMed/NCBI

36 

Zhu Q, Chang Y, Yang J and Wei Q: Post-translational modifications of proliferating cell nuclear antigen: A key signal integrator for DNA damage response (Review). Oncol Lett. 7:1363–1369. 2014.PubMed/NCBI

37 

Calaf GM, Echiburú-Chau C, Wen G, Balajee AS and Roy D: Effect of curcumin on irradiated and estrogen-transformed human breast cell lines. Int J Oncol. 40:436–442. 2012.

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Ling H, He J, Tan H, Yi L, Liu F, Ji X, Wu Y, Hu H, Zeng X, Ai X, Ai X, et al: Identification of potential targets for differentiation in human leukemia cells induced by diallyl disulfide. Int J Oncol 50: 697-707, 2017.
APA
Ling, H., He, J., Tan, H., Yi, L., Liu, F., Ji, X. ... Su, Q. (2017). Identification of potential targets for differentiation in human leukemia cells induced by diallyl disulfide. International Journal of Oncology, 50, 697-707. https://doi.org/10.3892/ijo.2017.3839
MLA
Ling, H., He, J., Tan, H., Yi, L., Liu, F., Ji, X., Wu, Y., Hu, H., Zeng, X., Ai, X., Jiang, H., Su, Q."Identification of potential targets for differentiation in human leukemia cells induced by diallyl disulfide". International Journal of Oncology 50.2 (2017): 697-707.
Chicago
Ling, H., He, J., Tan, H., Yi, L., Liu, F., Ji, X., Wu, Y., Hu, H., Zeng, X., Ai, X., Jiang, H., Su, Q."Identification of potential targets for differentiation in human leukemia cells induced by diallyl disulfide". International Journal of Oncology 50, no. 2 (2017): 697-707. https://doi.org/10.3892/ijo.2017.3839
Copy and paste a formatted citation
x
Spandidos Publications style
Ling H, He J, Tan H, Yi L, Liu F, Ji X, Wu Y, Hu H, Zeng X, Ai X, Ai X, et al: Identification of potential targets for differentiation in human leukemia cells induced by diallyl disulfide. Int J Oncol 50: 697-707, 2017.
APA
Ling, H., He, J., Tan, H., Yi, L., Liu, F., Ji, X. ... Su, Q. (2017). Identification of potential targets for differentiation in human leukemia cells induced by diallyl disulfide. International Journal of Oncology, 50, 697-707. https://doi.org/10.3892/ijo.2017.3839
MLA
Ling, H., He, J., Tan, H., Yi, L., Liu, F., Ji, X., Wu, Y., Hu, H., Zeng, X., Ai, X., Jiang, H., Su, Q."Identification of potential targets for differentiation in human leukemia cells induced by diallyl disulfide". International Journal of Oncology 50.2 (2017): 697-707.
Chicago
Ling, H., He, J., Tan, H., Yi, L., Liu, F., Ji, X., Wu, Y., Hu, H., Zeng, X., Ai, X., Jiang, H., Su, Q."Identification of potential targets for differentiation in human leukemia cells induced by diallyl disulfide". International Journal of Oncology 50, no. 2 (2017): 697-707. https://doi.org/10.3892/ijo.2017.3839
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team