Chondroitin sulfate proteoglycan protein is stimulated by interleukin 11 and promotes endometrial epithelial cancer cell proliferation and migration

  • Authors:
    • Amy Winship
    • Michelle Van Sinderen
    • Ariella Heffernan-Marks
    • Eva Dimitriadis
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  • Published online on: January 13, 2017     https://doi.org/10.3892/ijo.2017.3848
  • Pages: 798-804
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Abstract

Endometrial cancer is the most common gynecological cancer. We identified interleukin 11 (IL11) as a critical mediator of endometrial tumourigenesis and demonstrated that IL11 regulates chondroitin sulfate proteoglycan (CSPG4) in human placental trophoblasts. CSPG4 is a cell membrane protein overexpressed in numerous human cancers, although its role in endometrial cancer has not been investigated. We examined CSPG4 expression and localization in primary human type I endometrioid grade (G) 1-3 tumours by qPCR and immunohistochemistry and determined whether IL11 stimulated CSPG4. IL11 upregulated CSPG4 mRNA in HEC1A (G2-derived endometrial epithelial cancer cell line) cells. IL11 administration to BALB/c nude mice enhanced HEC1A xenograft tumour growth and increased CSPG4 protein in tumours. CSPG4 mRNA was unchanged between human G1-3 endometrial cancer and control tissues. CSPG4 protein levels were elevated in the epithelium of G2 and G3 endometrial cancer and in the tumour-associated stroma of G3 tumour tissues compared to proliferative phase or post-menopausal endometrium. CSPG4 knockdown by siRNA reduced HEC1A proliferation and migration in vitro and reduced gene expression of the key epithelial-to-mesenchymal transition (EMT) regulator SNAIL. Our data suggest that CSPG4 inhibition may impair endometrial cancer progression by reducing cancer cell proliferation, migration and potentially EMT.
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March-2017
Volume 50 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Winship A, Van Sinderen M, Heffernan-Marks A and Dimitriadis E: Chondroitin sulfate proteoglycan protein is stimulated by interleukin 11 and promotes endometrial epithelial cancer cell proliferation and migration. Int J Oncol 50: 798-804, 2017
APA
Winship, A., Van Sinderen, M., Heffernan-Marks, A., & Dimitriadis, E. (2017). Chondroitin sulfate proteoglycan protein is stimulated by interleukin 11 and promotes endometrial epithelial cancer cell proliferation and migration. International Journal of Oncology, 50, 798-804. https://doi.org/10.3892/ijo.2017.3848
MLA
Winship, A., Van Sinderen, M., Heffernan-Marks, A., Dimitriadis, E."Chondroitin sulfate proteoglycan protein is stimulated by interleukin 11 and promotes endometrial epithelial cancer cell proliferation and migration". International Journal of Oncology 50.3 (2017): 798-804.
Chicago
Winship, A., Van Sinderen, M., Heffernan-Marks, A., Dimitriadis, E."Chondroitin sulfate proteoglycan protein is stimulated by interleukin 11 and promotes endometrial epithelial cancer cell proliferation and migration". International Journal of Oncology 50, no. 3 (2017): 798-804. https://doi.org/10.3892/ijo.2017.3848