Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Oncology
Join Editorial Board Propose a Special Issue
Print ISSN: 1019-6439 Online ISSN: 1791-2423
Journal Cover
March-2017 Volume 50 Issue 3

Full Size Image

Cover Legend PDF

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March-2017 Volume 50 Issue 3

Full Size Image

Cover Legend PDF

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

HIST1H3D: A promising therapeutic target for lung cancer

  • Authors:
    • Yan Rui
    • Wen-Jia Peng
    • Ming Wang
    • Qian Wang
    • Zi-Li Liu
    • Yu-Qing Chen
    • Li-Nian Huang
  • View Affiliations / Copyright

    Affiliations: Department of Respiration and Critical Care Medicine, The First Affiliated Hospital of Bengbu Medical College, Lung Cancer Diagnosis and Treatment Center of Anhui Province, Anhui Provincial Key Laboratory of Clinical Basic Research on Respiratory Disease, Bengbu, Anhui 233004, P.R. China, Department of Epidemiology and Health Statistics, Bengbu Medical College, Bengbu, Anhui 233000, P.R. China
  • Pages: 815-822
    |
    Published online on: January 19, 2017
       https://doi.org/10.3892/ijo.2017.3856
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

HIST1H3D gene encodes histone H3.1 and is involved in gene-silencing and heterochromatin formation. HIST1H3D expression is upregulated in primary gastric cancer tissue. In this study, we explored the effects of HIST1H3D expression on lung cancer, and its mechanisms. HIST1H3D expression was measured by immunohistochemistry and RT-PCR in lung cancer tissues and human lung cancer cell lines. Cell proliferation was assessed by MTT assay. Flow cytometric analysis was used to determine cell cycle distribution and apoptosis. Levels of related proteins were detected by western blotting. Bioinformatics analysis was performed to investigate related signaling pathways. cDNA microarray analysis was performed to identify differentially expressed genes following HIST1H3D knockdown. HIST1H3D expression was upregulated in lung cancer tissue samples and the H1299 human lung cancer cell line (P<0.01). Regulation of HIST1H3D expression in nucleus of cells in lung cancer tissues was significant associated with tumor stage (P=0.02) and lymph node metastases (P=0.04). Downregulation of HIST1H3D expression led to suppression of proliferation and colony forming ability, cell cycle arrest at the G0/G1 phase, and promotion of cell apoptosis. The microarray data revealed 522 genes that were differentially expressed after HIST1H3D knockdown in H1299 cells. These genes were shown to be linked to numerous pathways, including the cell cycle, p53 signaling, and MCM. Western blot analysis confirmed upregulated expression of the THBS1 and TP53I3 genes, and downregulated expression of the CDK6, CDKN1 and CCNE2 genes. In conclusion, our results suggest that HIST1H3D is highly expressed in lung cancer cell lines and tissues. Furthermore, HIST1H3D may be important in cell proliferation, apoptosis and cell cycle progression, and is implicated as a potential therapeutic target for lung cancer.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Chen W, Li Z, Bai L and Lin Y: NF-kappaB in lung cancer, a carcinogenesis mediator and a prevention and therapy target. Front Biosci (Landmark Ed). 16:1172–1185. 2011. View Article : Google Scholar

3 

Vischioni B, Oudejans JJ, Vos W, Rodriguez JA and Giaccone G: Frequent overexpression of aurora B kinase, a novel drug target, in non-small cell lung carcinoma patients. Mol Cancer Ther. 5:2905–2913. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Birnstiel ML, Busslinger M and Strub K: Transcription termination and 3′ processing: The end is in site! Cell. 41:349–359. 1985. View Article : Google Scholar : PubMed/NCBI

5 

Liu TJ, Levine BJ, Skoultchi AI and Marzluff WF: The efficiency of 3′-end formation contributes to the relative levels of different histone mRNAs. Mol Cell Biol. 9:3499–3508. 1989. View Article : Google Scholar : PubMed/NCBI

6 

Osley MA: The regulation of histone synthesis in the cell cycle. Annu Rev Biochem. 60:827–861. 1991. View Article : Google Scholar : PubMed/NCBI

7 

Zanier K, Luyten I, Crombie C, Muller B, Schümperli D, Linge JP, Nilges M and Sattler M: Structure of the histone mRNA hairpin required for cell cycle regulation of histone gene expression. RNA. 8:29–46. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Meshi T, Taoka KI and Iwabuchi M: Regulation of histone gene expression during the cell cycle. Plant Mol Biol. 43:643–657. 2000. View Article : Google Scholar : PubMed/NCBI

9 

Schümperli D: Cell-cycle regulation of histone gene expression. Cell. 45:471–472. 1986. View Article : Google Scholar : PubMed/NCBI

10 

Turner BM: Cellular memory and the histone code. Cell. 111:285–291. 2002. View Article : Google Scholar : PubMed/NCBI

11 

Berger SL: Histone modifications in transcriptional regulation. Curr Opin Genet Dev. 12:142–148. 2002. View Article : Google Scholar : PubMed/NCBI

12 

Jenuwein T and Allis CD: Translating the histone code. Science. 293:1074–1080. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Cruft HJ, Mauritzen CM and Stedman E: Abnormal properties of histones from malignant cells. Nature. 174:580–585. 1954. View Article : Google Scholar : PubMed/NCBI

14 

Rheinbay E, Louis DN, Bernstein BE and Suvà ML: A tell-tail sign of chromatin: Histone mutations drive pediatric glioblastoma. Cancer Cell. 21:329–331. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Dryhurst D and Ausió J: Histone H2A.Z deregulation in prostate cancer. Cause or effect? Cancer Metastasis Rev. 33:429–439. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Albig W, Kioschis P, Poustka A, Meergans K and Doenecke D: Human histone gene organization: Nonregular arrangement within a large cluster. Genomics. 40:314–322. 1997. View Article : Google Scholar : PubMed/NCBI

17 

Peters AH and Schübeler D: Methylation of histones: Playing memory with DNA. Curr Opin Cell Biol. 17:230–238. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Iwaya T, Fukagawa T, Suzuki Y, Takahashi Y, Sawada G, Ishibashi M, Kurashige J, Sudo T, Tanaka F, Shibata K, et al: Contrasting expression patterns of histone mRNA and microRNA 760 in patients with gastric cancer. Clin Cancer Res. 19:6438–6449. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Wang B, Tang J, Liao D, Wang G, Zhang M, Sang Y, Cao J, Wu Y, Zhang R, Li S, et al: Chromobox homolog 4 is correlated with prognosis and tumor cell growth in hepatocellular carcinoma. Ann Surg Oncol. 20(Suppl 3): S684–S692. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Esteller M: Cancer epigenomics: DNA methylomes and histone-modification maps. Nat Rev Genet. 8:286–298. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Berdasco M and Esteller M: Aberrant epigenetic landscape in cancer: How cellular identity goes awry. Dev Cell. 19:698–711. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Turner G and Hancock RL: Histone methylase activity of adult, embryonic and neoplastic liver tissues. Life Sci II. 9:917–922. 1970. View Article : Google Scholar : PubMed/NCBI

23 

Ballestar E and Esteller M: The epigenetic breakdown of cancer cells: From DNA methylation to histone modifications. Prog Mol Subcell Biol. 38:169–181. 2005. View Article : Google Scholar : PubMed/NCBI

24 

Sakamoto R, Nitta T, Kamikawa Y, Sugihara K, Hasui K, Tsuyama S and Murata F: The assessment of cell proliferation during 9,10-dimethyl-1,2-benzanthracene-induced hamster tongue carcinogenesis by means of histone H3 mRNA in situ hybridization. Med Electron Microsc. 37:52–61. 2004. View Article : Google Scholar : PubMed/NCBI

25 

Piscopo M, Campisi G, Colella G, Bilancione M, Caccamo S, Di Liberto C, Tartaro GP, Giovannelli L, Pulcrano G and Fucci L: H3 and H3.3 histone mRNA amounts and ratio in oral squamous cell carcinoma and leukoplakia. Oral Dis. 12:130–136. 2006. View Article : Google Scholar : PubMed/NCBI

26 

Sadikovic B, Yoshimoto M, Chilton-MacNeill S, Thorner P, Squire JA and Zielenska M: Identification of interactive networks of gene expression associated with osteosarcoma oncogenesis by integrated molecular profiling. Hum Mol Genet. 18:1962–1975. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Pérez-Magán E, Rodríguez de Lope A, Ribalta T, Ruano Y, Campos-Martín Y, Pérez-Bautista G, García JF, García-Claver A, Fiaño C, Hernández-Moneo JL, et al: Differential expression profiling analyses identifies downregulation of 1p, 6q, and 14q genes and overexpression of 6p histone cluster 1 genes as markers of recurrence in meningiomas. Neuro Oncol. 12:1278–1290. 2010.PubMed/NCBI

28 

Sen GL and Blau HM: A brief history of RNAi: The silence of the genes. FASEB J. 20:1293–1299. 2006. View Article : Google Scholar : PubMed/NCBI

29 

Kong Q: RNAi: A novel strategy for the treatment of prion diseases. J Clin Invest. 116:3101–3103. 2006. View Article : Google Scholar : PubMed/NCBI

30 

Wu Z, Li G, Wu L, Weng D, Li X and Yao K: Cripto-1 overexpression is involved in the tumorigenesis of nasopharyngeal carcinoma. BMC Cancer. 9:3152009. View Article : Google Scholar : PubMed/NCBI

31 

Sumimoto H, Hirata K, Yamagata S, Miyoshi H, Miyagishi M, Taira K and Kawakami Y: Effective inhibition of cell growth and invasion of melanoma by combined suppression of BRAF (V599E) and Skp2 with lentiviral RNAi. Int J Cancer. 118:472–476. 2006. View Article : Google Scholar

32 

Vousden KH and Prives C: Blinded by the light: The growing complexity of p53. Cell. 137:413–431. 2009. View Article : Google Scholar : PubMed/NCBI

33 

Levine AJ and Oren M: The first 30 years of p53: Growing ever more complex. Nat Rev Cancer. 9:749–758. 2009. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Rui Y, Peng W, Wang M, Wang Q, Liu Z, Chen Y and Huang L: HIST1H3D: A promising therapeutic target for lung cancer. Int J Oncol 50: 815-822, 2017.
APA
Rui, Y., Peng, W., Wang, M., Wang, Q., Liu, Z., Chen, Y., & Huang, L. (2017). HIST1H3D: A promising therapeutic target for lung cancer. International Journal of Oncology, 50, 815-822. https://doi.org/10.3892/ijo.2017.3856
MLA
Rui, Y., Peng, W., Wang, M., Wang, Q., Liu, Z., Chen, Y., Huang, L."HIST1H3D: A promising therapeutic target for lung cancer". International Journal of Oncology 50.3 (2017): 815-822.
Chicago
Rui, Y., Peng, W., Wang, M., Wang, Q., Liu, Z., Chen, Y., Huang, L."HIST1H3D: A promising therapeutic target for lung cancer". International Journal of Oncology 50, no. 3 (2017): 815-822. https://doi.org/10.3892/ijo.2017.3856
Copy and paste a formatted citation
x
Spandidos Publications style
Rui Y, Peng W, Wang M, Wang Q, Liu Z, Chen Y and Huang L: HIST1H3D: A promising therapeutic target for lung cancer. Int J Oncol 50: 815-822, 2017.
APA
Rui, Y., Peng, W., Wang, M., Wang, Q., Liu, Z., Chen, Y., & Huang, L. (2017). HIST1H3D: A promising therapeutic target for lung cancer. International Journal of Oncology, 50, 815-822. https://doi.org/10.3892/ijo.2017.3856
MLA
Rui, Y., Peng, W., Wang, M., Wang, Q., Liu, Z., Chen, Y., Huang, L."HIST1H3D: A promising therapeutic target for lung cancer". International Journal of Oncology 50.3 (2017): 815-822.
Chicago
Rui, Y., Peng, W., Wang, M., Wang, Q., Liu, Z., Chen, Y., Huang, L."HIST1H3D: A promising therapeutic target for lung cancer". International Journal of Oncology 50, no. 3 (2017): 815-822. https://doi.org/10.3892/ijo.2017.3856
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team