Copy number analysis identifies tumor suppressive lncRNAs in human osteosarcoma

  • Authors:
    • Jianjun Xie
    • Dechen Lin
    • Dhong Hyun Tony Lee
    • Jennifer Akunowicz
    • Marc Hansen
    • Carl Miller
    • Masashi Sanada
    • Motohiro Kato
    • Tadayuki Akagi
    • Norihiko Kawamata
    • Seishi Ogawa
    • H. Phillip Koeffler
  • View Affiliations

  • Published online on: January 30, 2017     https://doi.org/10.3892/ijo.2017.3864
  • Pages: 863-872
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Abstract

Osteosarcoma (OS) has a high degree of chromosomal instability and total copy number (CN) changes. We examined 58 human OS samples including 40 primary tumors, 11 explants, and 7 cell lines using single nucleotide polymorphism (SNP) arrays, and revealed that 70% of the samples had one or more recurrent CN-neutral loss of heterozygosity (CNN‑LOH) also known as uniparental disomy (UPD). Importantly, 17% of the samples showed prominent homozygous deletion of 3q13.31, suggesting its role in tumorigenesis. We identified and characterized two novel lncRNAs, LOC285194 and BC040587, within this genomic locus, strongly suggesting their tumor suppressor activity. Frequent deletions and UPD suggest that OS often has mutant or non-expressed tumor suppressor genes including two lncRNAs.
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March-2017
Volume 50 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Xie J, Lin D, Lee DT, Akunowicz J, Hansen M, Miller C, Sanada M, Kato M, Akagi T, Kawamata N, Kawamata N, et al: Copy number analysis identifies tumor suppressive lncRNAs in human osteosarcoma. Int J Oncol 50: 863-872, 2017
APA
Xie, J., Lin, D., Lee, D.T., Akunowicz, J., Hansen, M., Miller, C. ... Koeffler, H.P. (2017). Copy number analysis identifies tumor suppressive lncRNAs in human osteosarcoma. International Journal of Oncology, 50, 863-872. https://doi.org/10.3892/ijo.2017.3864
MLA
Xie, J., Lin, D., Lee, D. T., Akunowicz, J., Hansen, M., Miller, C., Sanada, M., Kato, M., Akagi, T., Kawamata, N., Ogawa, S., Koeffler, H. P."Copy number analysis identifies tumor suppressive lncRNAs in human osteosarcoma". International Journal of Oncology 50.3 (2017): 863-872.
Chicago
Xie, J., Lin, D., Lee, D. T., Akunowicz, J., Hansen, M., Miller, C., Sanada, M., Kato, M., Akagi, T., Kawamata, N., Ogawa, S., Koeffler, H. P."Copy number analysis identifies tumor suppressive lncRNAs in human osteosarcoma". International Journal of Oncology 50, no. 3 (2017): 863-872. https://doi.org/10.3892/ijo.2017.3864