PGRMC1-dependent autophagy by hyperoside induces apoptosis and sensitizes ovarian cancer cells to cisplatin treatment

  • Authors:
    • Xiaofei Zhu
    • Mingde Ji
    • Yue Han
    • Yuanyuan Guo
    • Wenqiang Zhu
    • Feng Gao
    • Xuewen Yang
    • Chunbing Zhang
  • View Affiliations

  • Published online on: February 10, 2017     https://doi.org/10.3892/ijo.2017.3873
  • Pages: 835-846
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Abstract

Cisplatin treatment some times leads to chemoresistance, which is now acknowledged partially due to the inductive expression of progesterone receptor membrane component (PGRMC)1 in ovarian cancer cells. PGRMC1 enhances autophagy, activates cytochrome p450, and inveigles signaling pathways to promote cell survival and reduce the effect of drug treatments. In this study, we give first line evidence that hyperoside inhibits cell viability, triggers autophagy and apoptosis in ovarian cancer cell lines. Mechanistically, PGRMC1-dependent autophagy was utilized by hyperoside to induce apoptotic cell death. Hyperoside induced the conversion of LC3B-I to LC3B-II and the formation of autophagosomes in ovarian cancer cells. Notably, PGRMC1 colocolized with LC3B‑II, and PGRMC1 overexpression enhanced hyperoside-induced autophagy and apoptosis, while PGRMC1 knockdown abrogated the action. Additionally, AKT signaling and Bcl-2 family were also involved in the hyperoside-induced autophagy and apoptosis. Importantly, in cisplatin-resistant ovarian cancer cells where PGRMC1 was overexpressed, hyperoside sensitized the cells to cisplatin treatment. Together these findings indicate hyperoside functions as a complementary therapy for ovarian cancer patients receiving platinum-based therapy.
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March-2017
Volume 50 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Zhu X, Ji M, Han Y, Guo Y, Zhu W, Gao F, Yang X and Zhang C: PGRMC1-dependent autophagy by hyperoside induces apoptosis and sensitizes ovarian cancer cells to cisplatin treatment. Int J Oncol 50: 835-846, 2017
APA
Zhu, X., Ji, M., Han, Y., Guo, Y., Zhu, W., Gao, F. ... Zhang, C. (2017). PGRMC1-dependent autophagy by hyperoside induces apoptosis and sensitizes ovarian cancer cells to cisplatin treatment. International Journal of Oncology, 50, 835-846. https://doi.org/10.3892/ijo.2017.3873
MLA
Zhu, X., Ji, M., Han, Y., Guo, Y., Zhu, W., Gao, F., Yang, X., Zhang, C."PGRMC1-dependent autophagy by hyperoside induces apoptosis and sensitizes ovarian cancer cells to cisplatin treatment". International Journal of Oncology 50.3 (2017): 835-846.
Chicago
Zhu, X., Ji, M., Han, Y., Guo, Y., Zhu, W., Gao, F., Yang, X., Zhang, C."PGRMC1-dependent autophagy by hyperoside induces apoptosis and sensitizes ovarian cancer cells to cisplatin treatment". International Journal of Oncology 50, no. 3 (2017): 835-846. https://doi.org/10.3892/ijo.2017.3873