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Article

Ectopic expression of the ATP synthase β subunit on the membrane of PC-3M cells supports its potential role in prostate cancer metastasis

  • Authors:
    • Wei Li
    • Yulin Li
    • Gaiyun Li
    • Zilong Zhou
    • Xiaona Chang
    • Yang Xia
    • Xinjie Dong
    • Zhijing Liu
    • Bo Ren
    • Wei Liu
    • Yilei Li
  • View Affiliations / Copyright

    Affiliations: The Key Laboratory of Pathobiology, Ministry of Education, The College of Basic Medical Sciences, Jilin University, Changchun, Jilin 130021, P.R. China, Department of Pathology, the Second Clinical Hospital, Jilin University, Changchun, Jilin 130021, P.R. China, Department of Pathology, the First Clinical Hospital, Jilin University, Changchun, Jilin 130021, P.R. China, Department of Pathology, Longgang District Central Hospital of Shenzhen, Shenzhen, Guangdong 518116, P.R. China
  • Pages: 1312-1320
    |
    Published online on: February 16, 2017
       https://doi.org/10.3892/ijo.2017.3878
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Abstract

Metastatic prostate cancer is associated with high mortality rates. Identification of metastasis-related proteins may facilitate the development of novel therapies for the treatment of metastatic disease. In the present study, we aimed to identify prostate cancer metastasis-associated membrane proteins. We developed a phage-displayed 7-mer peptide library to screen the target peptides that were specifically bound to PC-3M cells with subtractive panning from normal prostate cells and PC-3 prostate cancer cells. A novel short peptide (B04) was found to have high affinity to highly metastatic PC-3M cells. ATP synthase β subunit (ATP5B) was then identified as a binding partner of B04 on the PC-3M cell surface. ATP5B was expressed on the PC-3M cell membrane and on highly malignant human prostate cancer specimens, as shown using multiple methodologies. Furthermore, ATP5B-positive gold particles were detected on the cellular and mitochondrial membranes by immunoelectromicroscopy. These results implied the possibility that ATP5B may translocate from the inner mitochondrial membrane to the outer surface of PC-3M cells. Additional analysis showed that incubation of B04 with PC-3M cells reduced the detection of ATP5B by western blotting and flow cytometry and significantly inhibited the proliferation, invasion and metastasis of PC-3M cells. In conclusion, ATP5B, as a binding partner of a metastasis-related short peptide (B04) on prostate cancer cells, is involved in promoting prostate cancer metastasis. In conclusion, ATP5B may be a promising biomarker and therapeutic target for highly metastatic malignancies.
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1 

Jemal A, Siegel R, Xu J and Ward E: Cancer statistics, 2010. CA Cancer J Clin. 60:277–300. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Hanahan D and Weinberg RA: Hallmarks of cancer: the next generation. Cell. 144:646–674. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Kauffman EC, Robinson VL, Stadler WM, Sokoloff MH and Rinker-Schaeffer CW: Metastasis suppression: The evolving role of metastasis suppressor genes for regulating cancer cell growth at the secondary site. J Urol. 169:1122–1133. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Hanash SM, Madoz-Gurpide J and Misek DE: Identification of novel targets for cancer therapy using expression proteomics. Leukemia. 16:478–485. 2002. View Article : Google Scholar : PubMed/NCBI

5 

Gao J, Gao Y, Ju Y, Yang J, Wu Q, Zhang J, Du X, Wang Z, Song Y, Li H, et al: Proteomics-based generation and characterization of monoclonal antibodies against human liver mitochondrial proteins. Proteomics. 6:427–437. 2006. View Article : Google Scholar

6 

Liu B, Huang L, Sihlbom C, Burlingame A and Marks JD: Towards proteome-wide production of monoclonal antibody by phage display. J Mol Biol. 315:1063–1073. 2002. View Article : Google Scholar : PubMed/NCBI

7 

Chi SL and Pizzo SV: Cell surface F1Fo ATP synthase: A new paradigm? Ann Med. 38:429–438. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Lu ZJ, Song QF, Jiang SS, Song Q, Wang W, Zhang GH, Kan B, Chen LJ, Yang JL, Luo F, et al: Identification of ATP synthase beta subunit (ATPB) on the cellsurface as a non-small cell lung cancer (NSCLC) associated antigen. BMC Cancer. 9:162009. View Article : Google Scholar

9 

Dowling P, Meleady P, Dowd A, Henry M, Glynn S and Clynes M: Proteomic analysis of isolated membrane fractions from superin- vasivecancer cells. Biochim Biophys Acta. 1774:93–101. 2007. View Article : Google Scholar

10 

Kim BW, Choo HJ, Lee JW, Kim JH and Ko YG: Extracellular ATP isgenerated by ATP synthase complex in adipocyte lipid rafts. Exp Mol Med. 36:476–485. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Hong D, Jaron D, Buerk DG and Barbee KA: Transport- dependent calcium signaling in spatially segregated cellular caveolar domains. Am J Physiol Cell Physiol. 294:C856–C866. 2008. View Article : Google Scholar

12 

Bae TJ, Kim MS, Kim JW, Kim BW, Choo HJ, Lee JW, Kim KB, Lee CS, Kim JH, Chang SY, et al: Lipid raft proteome reveals ATP synthase complex in the cell surface. Proteomics. 4:3536–3548. 2004. View Article : Google Scholar : PubMed/NCBI

13 

Ribatti D: Chicken chorioallantoic membrane angiogenesis model. Methods Mol Biol. 843:47–57. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Kariya Y, Kato K, Hayashizaki Y, Himeno S, Tarui S and Matsubura K: Revision of consensus sequence of human Alu repeats a review. Gene. 53:1–10. 1987. View Article : Google Scholar

15 

Boyer PD: The ATP synthase - a splendid molecular machine. Annu Rev Biochem. 66:717–749. 1997. View Article : Google Scholar

16 

Larimer BM, Thomas WD, Smith GP and Deutscher SL: Affinity maturation of an ERBB2-targeted SPECT imaging peptide by in vivo phage display. Mol Imaging Biol. 16:449e582014. View Article : Google Scholar

17 

Goetz M and Wang TD: Molecular imaging in gastrointestinal endoscopy. Gastroenterology. 138:828e33.e8212010. View Article : Google Scholar

18 

Senior AE: ATP synthesis by oxidative phosphorylation. Physiol Rev. 68:177–231. 1988.PubMed/NCBI

19 

Stock D, Leslie AG and Walker JE: Molecular architecture of the rotary motor in ATP synthase. Science. 286:1700–1705. 1999. View Article : Google Scholar : PubMed/NCBI

20 

Ma Z, Cao M, Liu Y, He Y, Wang Y, Yang C, Wang W, Du Y, Zhou M and Gao F: Mitochondrial F1Fo-ATP synthase translocates to cell surface in hepatocytes and has high activity in tumor-like acidic and hypoxic environment. Acta Biochim Biophys Sin (Shanghai). 42:530–537. 2010. View Article : Google Scholar

21 

Champagne E, Martinez LO and Collet Xand Barbaras R: Ecto-F1Fo ATP synthase/F1 ATPase: metabolic and immunological functions. Curr Opin Lipidol. 17:279–284. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Jung KH, Song SH, Paik JY, Koh BH, Choe YS, Lee EJ, Kim BT and Lee KH: Direct targeting of tumor cell F1F0 ATP-synthase by radioiodine angiostatin in vitro and in vivo. Cancer Biother Radiopharm. 22:704–712. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Das B, Mondragon MO, Sadeghian M, Hatcher VB and Norin AJ: A novel ligand in lymphocyte-mediated cytotoxicity: Expression of the beta subunit of H+ transporting ATP synthase on the surface of tumor cell lines. J Exp Med. 180:273–281. 1994. View Article : Google Scholar : PubMed/NCBI

24 

Arakaki N, Nagao T, Niki R, Toyofuku A, Tanaka H, Kuramoto Y, Emoto Y, Shibata H, Magota K and Higuti T: Possible role of cell surface H -ATP synthase in the extracellular ATP synthesis and proliferation of human umbilical vein endothelial cells. Mol Cancer Res. 1:931–939. 2003.PubMed/NCBI

25 

Moser TL, Stack MS, Asplin I, Enghild JJ, Højrup P, Everitt L, Hubchak S, Schnaper HW and Pizzo SV: Angiostatin binds ATP synthase on the surface of human endothelial cells. Proc Natl Acad Sci USA. 96:2811–2816. 1999. View Article : Google Scholar : PubMed/NCBI

26 

Moser TL, Kenan DJ, Ashley TA, Roy JA, Goodman MD, Misra UK, Cheek DJ and Pizzo SV: Endothelial cell surface F1-F0 ATP synthase is active in ATP synthesis and is inhibited by angiostatin. Proc Natl Acad Sci USA. 98:6656–6661. 2001. View Article : Google Scholar : PubMed/NCBI

27 

Notari L, Arakaki N, Mueller D, Meier S, Amaral J and Becerra SP: Pigment epithelium-derived factor binds to cell- surface F1-ATP synthase. FEBS J. 277:2192–2205. 2010. View Article : Google Scholar : PubMed/NCBI

28 

Rumjahn SM, Yokdang N, Baldwin KA, Thai J and Buxton IL: Purinergic regulation of vascular endothelial growth factor signaling in angiogenesis. Br J Cancer. 100:1465–1470. 2009. View Article : Google Scholar : PubMed/NCBI

29 

Chi SL and Pizzo SV: Angiostatin is directly cytotoxic to tumor cells at low extracellular pH: A mechanism dependent on cell surface-associated ATP synthase. Cancer Res. 66:875–882. 2006. View Article : Google Scholar : PubMed/NCBI

30 

Schwiebert EM and Zsembery A: Extracellular ATP as a signaling molecule for epithelial cells. Biochim Biophys Acta. 1615:7–32. 2003. View Article : Google Scholar : PubMed/NCBI

31 

Bodin P and Burnstock G: Increased release of ATP from endothelial cells during acute inflammation. Inflamm Res. 47:351–354. 1998. View Article : Google Scholar : PubMed/NCBI

32 

Di Virgilio F: Dr. Jekyll/Mr. Hyde: The dual role of extracellular ATP. J Auton Nerv Syst. 81:59–63. 2000. View Article : Google Scholar : PubMed/NCBI

33 

Ralevic V and Burnstock G: Receptors for purines and pyrimidines. Pharmacol Rev. 50:413–492. 1998.PubMed/NCBI

34 

Wen LT and Knowles AF: Extracellular ATP and adenosine induce cell apoptosis of human hepatoma Li-7A cells via the A3 adenosine receptor. Br J Pharmacol. 140:1009–1018. 2003. View Article : Google Scholar : PubMed/NCBI

35 

Schwiebert LM, Rice WC, Kudlow BA, Taylor AL and Schwiebert EM: Extracellular ATP signaling and P2X nucleotide receptors in monolayers of primary human vascular endothelial cells. Am J Physiol Cell Physiol. 282:C289–C301. 2002. View Article : Google Scholar : PubMed/NCBI

36 

Burnstock G: Purinergic signaling and vascular cell proliferation and death. Arterioscler Thromb Vasc Biol. 22:364–373. 2002. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li W, Li Y, Li G, Zhou Z, Chang X, Xia Y, Dong X, Liu Z, Ren B, Liu W, Liu W, et al: Ectopic expression of the ATP synthase β subunit on the membrane of PC-3M cells supports its potential role in prostate cancer metastasis. Int J Oncol 50: 1312-1320, 2017.
APA
Li, W., Li, Y., Li, G., Zhou, Z., Chang, X., Xia, Y. ... Li, Y. (2017). Ectopic expression of the ATP synthase β subunit on the membrane of PC-3M cells supports its potential role in prostate cancer metastasis. International Journal of Oncology, 50, 1312-1320. https://doi.org/10.3892/ijo.2017.3878
MLA
Li, W., Li, Y., Li, G., Zhou, Z., Chang, X., Xia, Y., Dong, X., Liu, Z., Ren, B., Liu, W., Li, Y."Ectopic expression of the ATP synthase β subunit on the membrane of PC-3M cells supports its potential role in prostate cancer metastasis". International Journal of Oncology 50.4 (2017): 1312-1320.
Chicago
Li, W., Li, Y., Li, G., Zhou, Z., Chang, X., Xia, Y., Dong, X., Liu, Z., Ren, B., Liu, W., Li, Y."Ectopic expression of the ATP synthase β subunit on the membrane of PC-3M cells supports its potential role in prostate cancer metastasis". International Journal of Oncology 50, no. 4 (2017): 1312-1320. https://doi.org/10.3892/ijo.2017.3878
Copy and paste a formatted citation
x
Spandidos Publications style
Li W, Li Y, Li G, Zhou Z, Chang X, Xia Y, Dong X, Liu Z, Ren B, Liu W, Liu W, et al: Ectopic expression of the ATP synthase β subunit on the membrane of PC-3M cells supports its potential role in prostate cancer metastasis. Int J Oncol 50: 1312-1320, 2017.
APA
Li, W., Li, Y., Li, G., Zhou, Z., Chang, X., Xia, Y. ... Li, Y. (2017). Ectopic expression of the ATP synthase β subunit on the membrane of PC-3M cells supports its potential role in prostate cancer metastasis. International Journal of Oncology, 50, 1312-1320. https://doi.org/10.3892/ijo.2017.3878
MLA
Li, W., Li, Y., Li, G., Zhou, Z., Chang, X., Xia, Y., Dong, X., Liu, Z., Ren, B., Liu, W., Li, Y."Ectopic expression of the ATP synthase β subunit on the membrane of PC-3M cells supports its potential role in prostate cancer metastasis". International Journal of Oncology 50.4 (2017): 1312-1320.
Chicago
Li, W., Li, Y., Li, G., Zhou, Z., Chang, X., Xia, Y., Dong, X., Liu, Z., Ren, B., Liu, W., Li, Y."Ectopic expression of the ATP synthase β subunit on the membrane of PC-3M cells supports its potential role in prostate cancer metastasis". International Journal of Oncology 50, no. 4 (2017): 1312-1320. https://doi.org/10.3892/ijo.2017.3878
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