Curcumin reverses benzidine-induced epithelial-mesenchymal transition via suppression of ERK5/AP-1 in SV-40 immortalized human urothelial cells

  • Authors:
    • Zhiqi Liu
    • Jie Liu
    • Li Zhao
    • Hao Geng
    • Jiaxing Ma
    • Zhiqiang Zhang
    • Dexin Yu
    • Caiyun Zhong
  • View Affiliations

  • Published online on: February 21, 2017     https://doi.org/10.3892/ijo.2017.3887
  • Pages: 1321-1329
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Abstract

Overexposure to benzidine has been manifested as an important cause of bladder cancer. However, the molecular mechanism of benzidine-induced malignancy is still insufficiently interpreted. Epithelial-mesenchymal transition (EMT) is a crucial pathophysiological process in embryonic development as well as initiation and development of epithelium-originated malignant tumors. The role of extracellular regulated protein kinase 5 (ERK5) in benzidine-meditated bladder cancer development has not been explored. In the present study, we explored the role of ERK5/AP-1 pathway in benzidine-induced EMT in human normal urothelial cells and the intervention effect of curcumin on bezidine-induced EMT. We found that benzidine-induced EMT in SV-40 immortalized human urothelial cells (SV-HUC-1) at low concentrations. We detected that ERK5/AP-1 pathway was notably activated. Specific ERK5 inhibitor, XMD8-92 was applied to determine the role of ERK5 in benzidine-induced EMT. Results indicated that XMD8-92 reversed the EMT process. Furthermore, curcumin effectively attenuated benzidine-induced urocystic EMT by suppressing ERK5/AP-1 pathway. In conclusion, the present study revealed the positive role of ERK5/AP-1 in benzidine-provoked urocystic EMT and the curcumin promising use in bladder cancer prevention and intervention via ERK5/AP-1 pathway.
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April-2017
Volume 50 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Liu Z, Liu J, Zhao L, Geng H, Ma J, Zhang Z, Yu D and Zhong C: Curcumin reverses benzidine-induced epithelial-mesenchymal transition via suppression of ERK5/AP-1 in SV-40 immortalized human urothelial cells. Int J Oncol 50: 1321-1329, 2017
APA
Liu, Z., Liu, J., Zhao, L., Geng, H., Ma, J., Zhang, Z. ... Zhong, C. (2017). Curcumin reverses benzidine-induced epithelial-mesenchymal transition via suppression of ERK5/AP-1 in SV-40 immortalized human urothelial cells. International Journal of Oncology, 50, 1321-1329. https://doi.org/10.3892/ijo.2017.3887
MLA
Liu, Z., Liu, J., Zhao, L., Geng, H., Ma, J., Zhang, Z., Yu, D., Zhong, C."Curcumin reverses benzidine-induced epithelial-mesenchymal transition via suppression of ERK5/AP-1 in SV-40 immortalized human urothelial cells". International Journal of Oncology 50.4 (2017): 1321-1329.
Chicago
Liu, Z., Liu, J., Zhao, L., Geng, H., Ma, J., Zhang, Z., Yu, D., Zhong, C."Curcumin reverses benzidine-induced epithelial-mesenchymal transition via suppression of ERK5/AP-1 in SV-40 immortalized human urothelial cells". International Journal of Oncology 50, no. 4 (2017): 1321-1329. https://doi.org/10.3892/ijo.2017.3887