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Overexpression of miRNA-221 promotes cell proliferation by targeting the apoptotic protease activating factor-1 and indicates a poor prognosis in ovarian cancer

  • Authors:
    • Jie Li
    • Qiang Li
    • He Huang
    • Yinguang Li
    • Li Li
    • Wenhui Hou
    • Zeshan You
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology, The Eastern Hospital of the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510700, P.R. China, Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China, Department of Gynecology, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong 510000, P.R. China, Department of Gynecology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1087-1096
    |
    Published online on: March 7, 2017
       https://doi.org/10.3892/ijo.2017.3898
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Abstract

MicroRNAs are a class of small non-coding, endogenous RNAs involved in cancer development and progression. MicroRNA-221 (mir-221) has been reported to have both an oncogenic and tumor-suppressive role in human tumors, but the role of miR-221 in ovarian cancer is poorly understood. In the present study, the expression levels of miR-221 and the apoptosis protease activating factor 1 (APAF1) protein in 63 samples of ovarian cancer tissues and the cell lines, IOSE25, A2780, OVCAR3, SKOV3 and 3AO were detected by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and western blot analysis, respectively. Cell proliferation was measured using cell counting kit-8 (CCK-8); cell migration and invasion were detected using a transwell assay; cell apoptosis was evaluated by flow cytometry and hoechst staining, and a luciferase assay was performed to verify a putative target site of miR-221 in the 3'-UTR of APAF1 mRNA. Expression of miR-221 was upregulated in ovarian cancer tissues. Patients with increased miR-221 expression levels had a reduced disease-free survival (P=0.0014) and overall survival (P=0.0058) compared with those with low miR-221 expression. Transfection of SKOV3 and A2780 cell lines with miR-221 inhibitor induced APAF1 protein expression, suppressed cell proliferation and migration and promoted tumor cell apoptosis. In conclusion, the APAF1 gene was confirmed as a direct target of miR-221 and overexpression of APAF1 suppressed ovarian cancer cell proliferation and induced cell apoptosis in vitro. These findings indicate that miR-221-APAF1 should be studied further as a potential new diagnostic or prognostic biomarker for ovarian cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Li J, Li Q, Huang H, Li Y, Li L, Hou W and You Z: Overexpression of miRNA-221 promotes cell proliferation by targeting the apoptotic protease activating factor-1 and indicates a poor prognosis in ovarian cancer. Int J Oncol 50: 1087-1096, 2017.
APA
Li, J., Li, Q., Huang, H., Li, Y., Li, L., Hou, W., & You, Z. (2017). Overexpression of miRNA-221 promotes cell proliferation by targeting the apoptotic protease activating factor-1 and indicates a poor prognosis in ovarian cancer. International Journal of Oncology, 50, 1087-1096. https://doi.org/10.3892/ijo.2017.3898
MLA
Li, J., Li, Q., Huang, H., Li, Y., Li, L., Hou, W., You, Z."Overexpression of miRNA-221 promotes cell proliferation by targeting the apoptotic protease activating factor-1 and indicates a poor prognosis in ovarian cancer". International Journal of Oncology 50.4 (2017): 1087-1096.
Chicago
Li, J., Li, Q., Huang, H., Li, Y., Li, L., Hou, W., You, Z."Overexpression of miRNA-221 promotes cell proliferation by targeting the apoptotic protease activating factor-1 and indicates a poor prognosis in ovarian cancer". International Journal of Oncology 50, no. 4 (2017): 1087-1096. https://doi.org/10.3892/ijo.2017.3898
Copy and paste a formatted citation
x
Spandidos Publications style
Li J, Li Q, Huang H, Li Y, Li L, Hou W and You Z: Overexpression of miRNA-221 promotes cell proliferation by targeting the apoptotic protease activating factor-1 and indicates a poor prognosis in ovarian cancer. Int J Oncol 50: 1087-1096, 2017.
APA
Li, J., Li, Q., Huang, H., Li, Y., Li, L., Hou, W., & You, Z. (2017). Overexpression of miRNA-221 promotes cell proliferation by targeting the apoptotic protease activating factor-1 and indicates a poor prognosis in ovarian cancer. International Journal of Oncology, 50, 1087-1096. https://doi.org/10.3892/ijo.2017.3898
MLA
Li, J., Li, Q., Huang, H., Li, Y., Li, L., Hou, W., You, Z."Overexpression of miRNA-221 promotes cell proliferation by targeting the apoptotic protease activating factor-1 and indicates a poor prognosis in ovarian cancer". International Journal of Oncology 50.4 (2017): 1087-1096.
Chicago
Li, J., Li, Q., Huang, H., Li, Y., Li, L., Hou, W., You, Z."Overexpression of miRNA-221 promotes cell proliferation by targeting the apoptotic protease activating factor-1 and indicates a poor prognosis in ovarian cancer". International Journal of Oncology 50, no. 4 (2017): 1087-1096. https://doi.org/10.3892/ijo.2017.3898
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