HOTAIR upregulates an 18-gene cell cycle-related mRNA network in glioma

  • Authors:
    • Kai Huang
    • Jia Sun
    • Chao Yang
    • Yunfei Wang
    • Bingcong Zhou
    • Chunsheng Kang
    • Lei Han
    • Qixue Wang
  • View Affiliations

  • Published online on: March 7, 2017     https://doi.org/10.3892/ijo.2017.3901
  • Pages: 1271-1278
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Abstract

HOTAIR is a tumor promoting long non-coding RNA (lncRNA) with roles in multiple cancers. However, the role of HOTAIR in glioma has not been well charaterized. Genes that positively correlated with HOTAIR were identified from the Chinese Glioma Genome Atlas and constructed into an interacting network. In total, 18 genes with P-values <0.01 were further extracted and constructed into a subnetwork. Real-time PCR, western blot and immunofluorescence analyses were employed to examine the expression of the genes after HOTAIR overexpression or knockdown. Intracranial glioblastoma multiform (GBM) models were used to test the potential of HOTAIR as a glioma therapy target. It was discovered that the 18 genes that most significantly correlated with HOTAIR expression formed a cell cycle-related mRNA network, which is positively regulated by HOTAIR. Furthermore, HOTAIR knockdown inhibited mouse intracranial GBM model formation. HOTAIR positively regulates a cell cycle-related mRNA network in glioma, and could be a potential therapeutic target for treating glioma.
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April-2017
Volume 50 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Huang K, Sun J, Yang C, Wang Y, Zhou B, Kang C, Han L and Wang Q: HOTAIR upregulates an 18-gene cell cycle-related mRNA network in glioma. Int J Oncol 50: 1271-1278, 2017
APA
Huang, K., Sun, J., Yang, C., Wang, Y., Zhou, B., Kang, C. ... Wang, Q. (2017). HOTAIR upregulates an 18-gene cell cycle-related mRNA network in glioma. International Journal of Oncology, 50, 1271-1278. https://doi.org/10.3892/ijo.2017.3901
MLA
Huang, K., Sun, J., Yang, C., Wang, Y., Zhou, B., Kang, C., Han, L., Wang, Q."HOTAIR upregulates an 18-gene cell cycle-related mRNA network in glioma". International Journal of Oncology 50.4 (2017): 1271-1278.
Chicago
Huang, K., Sun, J., Yang, C., Wang, Y., Zhou, B., Kang, C., Han, L., Wang, Q."HOTAIR upregulates an 18-gene cell cycle-related mRNA network in glioma". International Journal of Oncology 50, no. 4 (2017): 1271-1278. https://doi.org/10.3892/ijo.2017.3901