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Deubiquitinating enzyme USP20 is a positive regulator of Claspin and suppresses the malignant characteristics of gastric cancer cells

  • Authors:
    • Chao Wang
    • Chen Yang
    • Jun Ji
    • Jinling Jiang
    • Min Shi
    • Qu Cai
    • Yingyan Yu
    • Zhenggang Zhu
    • Jun Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, P.R. China, Department of Surgery, Shanghai Key Laboratory of Gastric Neoplasms, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1136-1146
    |
    Published online on: March 8, 2017
       https://doi.org/10.3892/ijo.2017.3904
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Abstract

The aim of the present study was to investigate the clinical significance, the biological function and the mechanisms of USP20 in gastric cancer. The expression of USP20 in 89 pairs of primary gastric cancer and peritumoral gastric tissues specimens were measured by immunohistochemistry. The correlation of USP20 expression with the survival and the clinicopathological characteristics of patients were analyzed. Moreover, the underlying mechanisms of ectopic USP20 expression and its impact on GC cells were also investigated. We found that the expression of USP20 is relatively low in GC tissues and negatively correlated with tumor size, tumor invasion and TNM staging. High expression of USP20 in GC predicted longer survival. Experimentally, small interfering RNA-mediated knockdown of USP20 expression significantly promoted cell proliferation, accelerated G1-S phase transition and attenuated the autophagy activity. Overexpression of USP20 led to the inhibition of proliferation, G1-S cell cycle transition delay and autophagy activation. Mechanistically, we confirmed that silencing the expression of USP20 in GC cells could reduce Claspin protein levels without altering Claspin mRNA levels, which is involved in the antitumor activity of USP20. Furthermore, the expression level of Claspin was relatively higher in peritumoral tissue than that of GC tissues and higher expression of Claspin in GC was also correlated with good prognosis of patients. Given its pivotal role in gastric tumorigenesis and progression, USP20 functioned as the tumor suppressor in GC and possessed promising value to be a therapeutic target for GC.
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Copy and paste a formatted citation
Spandidos Publications style
Wang C, Yang C, Ji J, Jiang J, Shi M, Cai Q, Yu Y, Zhu Z and Zhang J: Deubiquitinating enzyme USP20 is a positive regulator of Claspin and suppresses the malignant characteristics of gastric cancer cells. Int J Oncol 50: 1136-1146, 2017.
APA
Wang, C., Yang, C., Ji, J., Jiang, J., Shi, M., Cai, Q. ... Zhang, J. (2017). Deubiquitinating enzyme USP20 is a positive regulator of Claspin and suppresses the malignant characteristics of gastric cancer cells. International Journal of Oncology, 50, 1136-1146. https://doi.org/10.3892/ijo.2017.3904
MLA
Wang, C., Yang, C., Ji, J., Jiang, J., Shi, M., Cai, Q., Yu, Y., Zhu, Z., Zhang, J."Deubiquitinating enzyme USP20 is a positive regulator of Claspin and suppresses the malignant characteristics of gastric cancer cells". International Journal of Oncology 50.4 (2017): 1136-1146.
Chicago
Wang, C., Yang, C., Ji, J., Jiang, J., Shi, M., Cai, Q., Yu, Y., Zhu, Z., Zhang, J."Deubiquitinating enzyme USP20 is a positive regulator of Claspin and suppresses the malignant characteristics of gastric cancer cells". International Journal of Oncology 50, no. 4 (2017): 1136-1146. https://doi.org/10.3892/ijo.2017.3904
Copy and paste a formatted citation
x
Spandidos Publications style
Wang C, Yang C, Ji J, Jiang J, Shi M, Cai Q, Yu Y, Zhu Z and Zhang J: Deubiquitinating enzyme USP20 is a positive regulator of Claspin and suppresses the malignant characteristics of gastric cancer cells. Int J Oncol 50: 1136-1146, 2017.
APA
Wang, C., Yang, C., Ji, J., Jiang, J., Shi, M., Cai, Q. ... Zhang, J. (2017). Deubiquitinating enzyme USP20 is a positive regulator of Claspin and suppresses the malignant characteristics of gastric cancer cells. International Journal of Oncology, 50, 1136-1146. https://doi.org/10.3892/ijo.2017.3904
MLA
Wang, C., Yang, C., Ji, J., Jiang, J., Shi, M., Cai, Q., Yu, Y., Zhu, Z., Zhang, J."Deubiquitinating enzyme USP20 is a positive regulator of Claspin and suppresses the malignant characteristics of gastric cancer cells". International Journal of Oncology 50.4 (2017): 1136-1146.
Chicago
Wang, C., Yang, C., Ji, J., Jiang, J., Shi, M., Cai, Q., Yu, Y., Zhu, Z., Zhang, J."Deubiquitinating enzyme USP20 is a positive regulator of Claspin and suppresses the malignant characteristics of gastric cancer cells". International Journal of Oncology 50, no. 4 (2017): 1136-1146. https://doi.org/10.3892/ijo.2017.3904
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