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Article

miR-145 inhibits proliferation and migration of breast cancer cells by directly or indirectly regulating TGF-β1 expression

  • Authors:
    • Yanling Ding
    • Chunfu Zhang
    • Jiahui Zhang
    • Nannan Zhang
    • Tao Li
    • Jie Fang
    • Yi Zhang
    • Feiyang Zuo
    • Zehua Tao
    • Shengnan Tang
    • Wei Zhu
    • Huabiao Chen
    • Xiaochun Sun
  • View Affiliations / Copyright

    Affiliations: School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, P.R. China, The Second People's Hospital of Kunshan, Kunshan, Jiangsu 215300, P.R. China, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, P.R. China
  • Pages: 1701-1710
    |
    Published online on: April 4, 2017
       https://doi.org/10.3892/ijo.2017.3945
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Abstract

Studies have demonstrated low expression of miR-145 associated with cell proliferation and migration in a wide variety of tumors. Here, we studied the expression of miR-145 in relation to the occurrence and development of breast cancer. Total RNA from breast cancer tissue and corresponding adjacent normal tissue was extracted and used to detect miR-145 expression by quantitative real-time polymerase chain reaction (qRT-PCR). We also transfected breast cancer cells with hsa-miR-145 mimics, hsa-miR-145 inhibitor, mimics negative control (mimics NC) or inhibitor negative control (inhibitor NC). Cell proliferation was analyzed by colony formation assays and methyl thiazolyl tetrazolium assays. Cell proliferation in breast cancer cells was decreased after overexpression of miR-145 and increased following miR-145 suppression. Cell migration and invasion were assessed using Transwell and wound healing assays, respectively, and were also decreased after overexpression of miR-145 and increased after miR-145 suppression in breast cancer cells. Finally, western blot assays showed that overexpression of miR-145 inhibited expression of transforming growth factor-β1 (TGF-β1). Collectively, these data suggest that miR-145 may inhibit TGF-β1 protein expression which may in turn contribute to tumor formation.
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Copy and paste a formatted citation
Spandidos Publications style
Ding Y, Zhang C, Zhang J, Zhang N, Li T, Fang J, Zhang Y, Zuo F, Tao Z, Tang S, Tang S, et al: miR-145 inhibits proliferation and migration of breast cancer cells by directly or indirectly regulating TGF-β1 expression. Int J Oncol 50: 1701-1710, 2017.
APA
Ding, Y., Zhang, C., Zhang, J., Zhang, N., Li, T., Fang, J. ... Sun, X. (2017). miR-145 inhibits proliferation and migration of breast cancer cells by directly or indirectly regulating TGF-β1 expression. International Journal of Oncology, 50, 1701-1710. https://doi.org/10.3892/ijo.2017.3945
MLA
Ding, Y., Zhang, C., Zhang, J., Zhang, N., Li, T., Fang, J., Zhang, Y., Zuo, F., Tao, Z., Tang, S., Zhu, W., Chen, H., Sun, X."miR-145 inhibits proliferation and migration of breast cancer cells by directly or indirectly regulating TGF-β1 expression". International Journal of Oncology 50.5 (2017): 1701-1710.
Chicago
Ding, Y., Zhang, C., Zhang, J., Zhang, N., Li, T., Fang, J., Zhang, Y., Zuo, F., Tao, Z., Tang, S., Zhu, W., Chen, H., Sun, X."miR-145 inhibits proliferation and migration of breast cancer cells by directly or indirectly regulating TGF-β1 expression". International Journal of Oncology 50, no. 5 (2017): 1701-1710. https://doi.org/10.3892/ijo.2017.3945
Copy and paste a formatted citation
x
Spandidos Publications style
Ding Y, Zhang C, Zhang J, Zhang N, Li T, Fang J, Zhang Y, Zuo F, Tao Z, Tang S, Tang S, et al: miR-145 inhibits proliferation and migration of breast cancer cells by directly or indirectly regulating TGF-β1 expression. Int J Oncol 50: 1701-1710, 2017.
APA
Ding, Y., Zhang, C., Zhang, J., Zhang, N., Li, T., Fang, J. ... Sun, X. (2017). miR-145 inhibits proliferation and migration of breast cancer cells by directly or indirectly regulating TGF-β1 expression. International Journal of Oncology, 50, 1701-1710. https://doi.org/10.3892/ijo.2017.3945
MLA
Ding, Y., Zhang, C., Zhang, J., Zhang, N., Li, T., Fang, J., Zhang, Y., Zuo, F., Tao, Z., Tang, S., Zhu, W., Chen, H., Sun, X."miR-145 inhibits proliferation and migration of breast cancer cells by directly or indirectly regulating TGF-β1 expression". International Journal of Oncology 50.5 (2017): 1701-1710.
Chicago
Ding, Y., Zhang, C., Zhang, J., Zhang, N., Li, T., Fang, J., Zhang, Y., Zuo, F., Tao, Z., Tang, S., Zhu, W., Chen, H., Sun, X."miR-145 inhibits proliferation and migration of breast cancer cells by directly or indirectly regulating TGF-β1 expression". International Journal of Oncology 50, no. 5 (2017): 1701-1710. https://doi.org/10.3892/ijo.2017.3945
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