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Article

Diverse expression patterns and tumorigenic role of neurotensin signaling components in colorectal cancer cells

  • Authors:
    • Ji Tae Kim
    • Heidi L. Weiss
    • B. Mark Evers
  • View Affiliations / Copyright

    Affiliations: Markey Cancer Center, University of Kentucky, Lexington, KY 40536, USA
  • Pages: 2200-2206
    |
    Published online on: May 9, 2017
       https://doi.org/10.3892/ijo.2017.3990
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Abstract

Colorectal cancer (CRC), which is one of the most common malignancies worldwide, results from an accumulation of genetic and epigenetic modifications including DNA methylation. Neurotensin (NTS), a hormone localized to the gut and central nervous system, mediates its physiological and pathological effects, including growth stimulation for a variety of cancers, through three distinct NTS receptors (NTSRs). Most NTS functions are mediated through the high-affinity receptor NTSR1, and expression of NTSR1 is increased in many cancers including CRC. In this study, we investigated the expression profiles and cellular functions of the NTSRs, especially NTSR1, in CRC cells. We showed that expression levels for NTS and NTSR1 varied, that NTSR2 expression was not detectable and that NTSR3 was consistently expressed in all CRC cell lines examined. Treatment with the demethylating agent, 5-aza-2'-deoxycytidine, augmented levels of NTSR1/2 in Caco2 and DLD1 cells, which have little or no transcripts for NTSR1/2 suggesting that DNA methylation suppresses NTSR1/2 expression. In addition, we demonstrated that knockdown of NTSR1 decreased cell growth and migration in HCT116 and HT29 cells. Finally, we showed that treatment with SR48692, an antagonist of NTSR1, also inhibited cell proliferation and migration in the CRC cells. Our findings identify promoter methylation as an important process regulating the differential expression or silencing of NTSR1/2 in CRC cells. Moreover, inhibition of NTSR1 repressed tumorigenic effects in CRC cells, suggesting that NTSR1 may be used as a therapeutic target for CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Kim JT, Weiss HL and Evers BM: Diverse expression patterns and tumorigenic role of neurotensin signaling components in colorectal cancer cells. Int J Oncol 50: 2200-2206, 2017.
APA
Kim, J.T., Weiss, H.L., & Evers, B.M. (2017). Diverse expression patterns and tumorigenic role of neurotensin signaling components in colorectal cancer cells. International Journal of Oncology, 50, 2200-2206. https://doi.org/10.3892/ijo.2017.3990
MLA
Kim, J. T., Weiss, H. L., Evers, B. M."Diverse expression patterns and tumorigenic role of neurotensin signaling components in colorectal cancer cells". International Journal of Oncology 50.6 (2017): 2200-2206.
Chicago
Kim, J. T., Weiss, H. L., Evers, B. M."Diverse expression patterns and tumorigenic role of neurotensin signaling components in colorectal cancer cells". International Journal of Oncology 50, no. 6 (2017): 2200-2206. https://doi.org/10.3892/ijo.2017.3990
Copy and paste a formatted citation
x
Spandidos Publications style
Kim JT, Weiss HL and Evers BM: Diverse expression patterns and tumorigenic role of neurotensin signaling components in colorectal cancer cells. Int J Oncol 50: 2200-2206, 2017.
APA
Kim, J.T., Weiss, H.L., & Evers, B.M. (2017). Diverse expression patterns and tumorigenic role of neurotensin signaling components in colorectal cancer cells. International Journal of Oncology, 50, 2200-2206. https://doi.org/10.3892/ijo.2017.3990
MLA
Kim, J. T., Weiss, H. L., Evers, B. M."Diverse expression patterns and tumorigenic role of neurotensin signaling components in colorectal cancer cells". International Journal of Oncology 50.6 (2017): 2200-2206.
Chicago
Kim, J. T., Weiss, H. L., Evers, B. M."Diverse expression patterns and tumorigenic role of neurotensin signaling components in colorectal cancer cells". International Journal of Oncology 50, no. 6 (2017): 2200-2206. https://doi.org/10.3892/ijo.2017.3990
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