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Article

TAZ overexpression is associated with epithelial-mesenchymal transition in cisplatin-resistant gastric cancer cells

  • Authors:
    • Liang Ge
    • Dong-Song Li
    • Fei Chen
    • Ji-Dong Feng
    • Bai Li
    • Tie-Jun Wang
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Abdominal Tumor Medical Department, Jilin Provincial Tumor Hospital, Changchun, Jilin 130021, P.R. China, Department of Normal Surgery, Jilin Province Hospital of Traditional Chinese Medicine, Changchun, Jilin 130021, P.R. China, Department of Colorectal and Anal Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Orthopedic Traumatology, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
  • Pages: 307-315
    |
    Published online on: May 16, 2017
       https://doi.org/10.3892/ijo.2017.3998
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Abstract

Gastric cancer is one of the common malignant diseases. The poor treatment outcome is mainly due to chemotherapeutic resistance. Therefore, it is important to determine the molecular mechanism of drug resistance in gastric cancer. To explore the mechanisms of cisplatin resistance in gastric cancer cells, several approaches were performed including MTT assay, real-time RT-PCR, western blot analysis, migration and invasion assays, wound healing assay, and transfection. We found that cisplatin-resistant (CR) gastric cancer cells acquired epithelial-mesenchymal transition (EMT) phenotype. The CR cells with EMT features obtained higher migratory and invasive activities. Moreover, we observed that TAZ was highly expressed in CR cells. Consistently, depletion of TAZ caused partial reversal of EMT to MET in CR cells. Our results suggest that TAZ plays a pivotal role in CR-induced EMT. Targeting TAZ could be a potential therapeutic strategy for gastric cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Ge L, Li D, Chen F, Feng J, Li B and Wang T: TAZ overexpression is associated with epithelial-mesenchymal transition in cisplatin-resistant gastric cancer cells. Int J Oncol 51: 307-315, 2017.
APA
Ge, L., Li, D., Chen, F., Feng, J., Li, B., & Wang, T. (2017). TAZ overexpression is associated with epithelial-mesenchymal transition in cisplatin-resistant gastric cancer cells. International Journal of Oncology, 51, 307-315. https://doi.org/10.3892/ijo.2017.3998
MLA
Ge, L., Li, D., Chen, F., Feng, J., Li, B., Wang, T."TAZ overexpression is associated with epithelial-mesenchymal transition in cisplatin-resistant gastric cancer cells". International Journal of Oncology 51.1 (2017): 307-315.
Chicago
Ge, L., Li, D., Chen, F., Feng, J., Li, B., Wang, T."TAZ overexpression is associated with epithelial-mesenchymal transition in cisplatin-resistant gastric cancer cells". International Journal of Oncology 51, no. 1 (2017): 307-315. https://doi.org/10.3892/ijo.2017.3998
Copy and paste a formatted citation
x
Spandidos Publications style
Ge L, Li D, Chen F, Feng J, Li B and Wang T: TAZ overexpression is associated with epithelial-mesenchymal transition in cisplatin-resistant gastric cancer cells. Int J Oncol 51: 307-315, 2017.
APA
Ge, L., Li, D., Chen, F., Feng, J., Li, B., & Wang, T. (2017). TAZ overexpression is associated with epithelial-mesenchymal transition in cisplatin-resistant gastric cancer cells. International Journal of Oncology, 51, 307-315. https://doi.org/10.3892/ijo.2017.3998
MLA
Ge, L., Li, D., Chen, F., Feng, J., Li, B., Wang, T."TAZ overexpression is associated with epithelial-mesenchymal transition in cisplatin-resistant gastric cancer cells". International Journal of Oncology 51.1 (2017): 307-315.
Chicago
Ge, L., Li, D., Chen, F., Feng, J., Li, B., Wang, T."TAZ overexpression is associated with epithelial-mesenchymal transition in cisplatin-resistant gastric cancer cells". International Journal of Oncology 51, no. 1 (2017): 307-315. https://doi.org/10.3892/ijo.2017.3998
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