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Metadherin regulates actin cytoskeletal remodeling and enhances human gastric cancer metastasis via epithelial-mesenchymal transition

  • Authors:
    • Yaqiong Du
    • Bojian Jiang
    • Shuzheng Song
    • Guoqing Pei
    • Xiaochun Ni
    • Jugang Wu
    • Shoulian Wang
    • Zhengyuan Wang
    • Jiwei Yu
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Baoshang, Shanghai 201999, P.R. China, Department of Breast Surgery, Yangpu Hospital, School of Medicine, Tongji University, Yangpu, Shanghai 200090, P.R. China
    Copyright: © Du et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 63-74
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    Published online on: May 17, 2017
       https://doi.org/10.3892/ijo.2017.4002
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Abstract

Metadherin (MTDH) can be recruited to mature tight junction complexes, and it regulates mesenchymal marker protein expression in many tumors and promote cancer metastasis. This study investigated the influence of MTDH expression on gastric cancer and to elucidate the potential mechanisms by which MTDH regulates actin cytoskeletal remodeling and enhances human gastric cancer metastasis via epithelial-mesenchymal transition (EMT). Relative MTDH mRNA expression levels were assessed by quantitative real-time PCR (Q-PCR), and MTDH protein expression levels and localization were evaluated via immunohistochemical (ICH) staining. We studied the role of MTDH in cancer cell migration and invasion by modulating MTDH expression in the gastric cancer cell lines MKN45 and AGS. We also confirmed the functions of MTDH through in vivo experiments. We found that MTDH expression levels were correlated with lymph node metastasis, TNM stages and decreased OS (P=0.002, <0.001 and 0.010, respectively) in human gastric cancer and that MTDH upregulation promoted EMT in vitro. Consistent with this finding, MTDH downregulation inhibited cell migration and invasion in vitro and suppressed tumor growth and metastasis in vivo. Furthermore, MTDH knockdown regulated actin cytoskeletal remodeling and inhibited EMT. Overall, our results provide a novel role for MTDH in regulating gastric cancer metastasis.
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Copy and paste a formatted citation
Spandidos Publications style
Du Y, Jiang B, Song S, Pei G, Ni X, Wu J, Wang S, Wang Z and Yu J: Metadherin regulates actin cytoskeletal remodeling and enhances human gastric cancer metastasis via epithelial-mesenchymal transition. Int J Oncol 51: 63-74, 2017.
APA
Du, Y., Jiang, B., Song, S., Pei, G., Ni, X., Wu, J. ... Yu, J. (2017). Metadherin regulates actin cytoskeletal remodeling and enhances human gastric cancer metastasis via epithelial-mesenchymal transition. International Journal of Oncology, 51, 63-74. https://doi.org/10.3892/ijo.2017.4002
MLA
Du, Y., Jiang, B., Song, S., Pei, G., Ni, X., Wu, J., Wang, S., Wang, Z., Yu, J."Metadherin regulates actin cytoskeletal remodeling and enhances human gastric cancer metastasis via epithelial-mesenchymal transition". International Journal of Oncology 51.1 (2017): 63-74.
Chicago
Du, Y., Jiang, B., Song, S., Pei, G., Ni, X., Wu, J., Wang, S., Wang, Z., Yu, J."Metadherin regulates actin cytoskeletal remodeling and enhances human gastric cancer metastasis via epithelial-mesenchymal transition". International Journal of Oncology 51, no. 1 (2017): 63-74. https://doi.org/10.3892/ijo.2017.4002
Copy and paste a formatted citation
x
Spandidos Publications style
Du Y, Jiang B, Song S, Pei G, Ni X, Wu J, Wang S, Wang Z and Yu J: Metadherin regulates actin cytoskeletal remodeling and enhances human gastric cancer metastasis via epithelial-mesenchymal transition. Int J Oncol 51: 63-74, 2017.
APA
Du, Y., Jiang, B., Song, S., Pei, G., Ni, X., Wu, J. ... Yu, J. (2017). Metadherin regulates actin cytoskeletal remodeling and enhances human gastric cancer metastasis via epithelial-mesenchymal transition. International Journal of Oncology, 51, 63-74. https://doi.org/10.3892/ijo.2017.4002
MLA
Du, Y., Jiang, B., Song, S., Pei, G., Ni, X., Wu, J., Wang, S., Wang, Z., Yu, J."Metadherin regulates actin cytoskeletal remodeling and enhances human gastric cancer metastasis via epithelial-mesenchymal transition". International Journal of Oncology 51.1 (2017): 63-74.
Chicago
Du, Y., Jiang, B., Song, S., Pei, G., Ni, X., Wu, J., Wang, S., Wang, Z., Yu, J."Metadherin regulates actin cytoskeletal remodeling and enhances human gastric cancer metastasis via epithelial-mesenchymal transition". International Journal of Oncology 51, no. 1 (2017): 63-74. https://doi.org/10.3892/ijo.2017.4002
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