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miR‑181b‑5p mediates TGF‑β1-induced epithelial-to-mesenchymal transition in non-small cell lung cancer stem-like cells derived from lung adenocarcinoma A549 cells

  • Authors:
    • Xuetao Li
    • Jing Han
    • Haizhen Zhu
    • Lina Peng
    • Zhengtang Chen
  • View Affiliations / Copyright

    Affiliations: Cancer Institute of PLA, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, P.R. China, Department of Oncology, Guizhou Provincial People's Hospital, Guiyang, Guizhou 550002, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 158-168
    |
    Published online on: May 17, 2017
       https://doi.org/10.3892/ijo.2017.4007
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Abstract

The ability of non-small cell lung cancer (NSCLC) cells to invade and metastasize is associated with epithelial-to-mesenchymal transition (EMT). The process of EMT is, at least in part, regulated by microRNAs. However, it is unknown whether microRNAs regulate EMT in cancer stem-like cells (CSLCs), or which microRNAs are involved. In the present study, we compared microRNA expression in A549 cells, TGF‑β1-treated A549 cells, CSLCs characterized by the CD133+/CD326+ phenotype, and TGF‑β1-treated CSLCs. We found that miR‑181b‑5p expression was upregulated by TGF‑β1. Moreover, the overexpression of the miR‑181b‑5p in A549 cells and CD133+/CD326+ cells resulted in the downregulation of the E-cadherin and increased invasion and metastasis in vitro and in vivo. Accordingly, the knockdown of miR‑181b‑5p partially restored E-cadherin expression. These results suggest that miR‑181b‑5p regulates TGF‑β1-induced EMT by targeting E-cadherin not only in normal A549 cells but also in CD133+/CD326+ cells which have characteristics of CSLCs. Thus, miR‑181b‑5p represents a new therapeutic target in NSCLC.
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Copy and paste a formatted citation
Spandidos Publications style
Li X, Han J, Zhu H, Peng L and Chen Z: miR‑181b‑5p mediates TGF‑β1-induced epithelial-to-mesenchymal transition in non-small cell lung cancer stem-like cells derived from lung adenocarcinoma A549 cells. Int J Oncol 51: 158-168, 2017.
APA
Li, X., Han, J., Zhu, H., Peng, L., & Chen, Z. (2017). miR‑181b‑5p mediates TGF‑β1-induced epithelial-to-mesenchymal transition in non-small cell lung cancer stem-like cells derived from lung adenocarcinoma A549 cells. International Journal of Oncology, 51, 158-168. https://doi.org/10.3892/ijo.2017.4007
MLA
Li, X., Han, J., Zhu, H., Peng, L., Chen, Z."miR‑181b‑5p mediates TGF‑β1-induced epithelial-to-mesenchymal transition in non-small cell lung cancer stem-like cells derived from lung adenocarcinoma A549 cells". International Journal of Oncology 51.1 (2017): 158-168.
Chicago
Li, X., Han, J., Zhu, H., Peng, L., Chen, Z."miR‑181b‑5p mediates TGF‑β1-induced epithelial-to-mesenchymal transition in non-small cell lung cancer stem-like cells derived from lung adenocarcinoma A549 cells". International Journal of Oncology 51, no. 1 (2017): 158-168. https://doi.org/10.3892/ijo.2017.4007
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Han J, Zhu H, Peng L and Chen Z: miR‑181b‑5p mediates TGF‑β1-induced epithelial-to-mesenchymal transition in non-small cell lung cancer stem-like cells derived from lung adenocarcinoma A549 cells. Int J Oncol 51: 158-168, 2017.
APA
Li, X., Han, J., Zhu, H., Peng, L., & Chen, Z. (2017). miR‑181b‑5p mediates TGF‑β1-induced epithelial-to-mesenchymal transition in non-small cell lung cancer stem-like cells derived from lung adenocarcinoma A549 cells. International Journal of Oncology, 51, 158-168. https://doi.org/10.3892/ijo.2017.4007
MLA
Li, X., Han, J., Zhu, H., Peng, L., Chen, Z."miR‑181b‑5p mediates TGF‑β1-induced epithelial-to-mesenchymal transition in non-small cell lung cancer stem-like cells derived from lung adenocarcinoma A549 cells". International Journal of Oncology 51.1 (2017): 158-168.
Chicago
Li, X., Han, J., Zhu, H., Peng, L., Chen, Z."miR‑181b‑5p mediates TGF‑β1-induced epithelial-to-mesenchymal transition in non-small cell lung cancer stem-like cells derived from lung adenocarcinoma A549 cells". International Journal of Oncology 51, no. 1 (2017): 158-168. https://doi.org/10.3892/ijo.2017.4007
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