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Article

MicroRNA-379 inhibits metastasis and epithelial-mesenchymal transition via targeting FAK/AKT signaling in gastric cancer

  • Authors:
    • Meizhong Xu
    • Shui Qin
    • Fan Cao
    • Shi Ding
    • Mengnan Li
  • View Affiliations / Copyright

    Affiliations: Forensic Surgery, The Traditional Chinese Medicine Hospital of Jingshan County, Jingshan, Hubei 431800, P.R. China, Department of Gastroenterology, The Traditional Chinese Medicine Hospital of Jingshan County, Jingshan, Hubei 431800, P.R. China
  • Pages: 867-876
    |
    Published online on: July 12, 2017
       https://doi.org/10.3892/ijo.2017.4072
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Abstract

Accumulating evidence demonstrates that aberrant miRNAs contribute to gastric cancer (GC) development and progression. However, the roles of various miRNAs in GC remain to be determined. In the present study, we confirmed that a reduced miR-379 expression was present in GC tissues and cell lines. Our clinical analysis revealed that the downregulated miR-379 expression was significantly correlated with poor prognostic features including lymph node metastasis and advanced TNM stage. Moreover, we confirmed that miR-379 was a novel independent prognostic marker for predicting 5-year survival of GC patients. The ectopic overexpression of miR-379 inhibited cell migration, invasion and EMT progress, while downregulated miR-379 reversed the effect. In addition, miR-379 regulated the focal adhesion kinase (FAK) by directly binding to its 3'-UTR, resulting in suppression of AKT signaling. In clinical samples of GC, miR-379 inversely correlated with FAK, which was upregulated in GC. Alteration of FAK expression or activating AKT signaling at least partially abolished the migration, invasion and EMT progress effects of miR-379 on GC cells. In conclusion, our results indicated that miR-379 functioned as a tumor suppressor gene in regulating the EMT and metastasis of GC via targeting FAK/AKT signaling, and may represent a novel potential therapeutic target and prognostic marker for GC.
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View References

1 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2015. CA Cancer J Clin. 65:5–29. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D and Bray F: Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 136:E359–E386. 2015. View Article : Google Scholar

3 

De Vita F, Vecchione L, Galizia G, Di Martino N, Fabozzi T, Catalano G, Ciardiello F and Orditura M: Perspectives in adjuvant therapy of gastric cancer. Oncology. 77(Suppl 1): 38–42. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Kagawa S, Shigeyasu K, Ishida M, Watanabe M, Tazawa H, Nagasaka T, Shirakawa Y and Fujiwara T: Molecular diagnosis and therapy for occult peritoneal metastasis in gastric cancer patients. World J Gastroenterol. 20:17796–17803. 2014.PubMed/NCBI

5 

Bessette DC, Qiu D and Pallen CJ: PRL PTPs: Mediators and markers of cancer progression. Cancer Metastasis Rev. 27:231–252. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Alvarez-Garcia I and Miska EA: MicroRNA functions in animal development and human disease. Development. 132:4653–4662. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Calin GA and Croce CM: MicroRNA-cancer connection: The beginning of a new tale. Cancer Res. 66:7390–7394. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Li C, Song L, Zhang Z, Bai XX, Cui MF and Ma LJ: MicroRNA-21 promotes TGF-β1-induced epithelial-mesenchymal transition in gastric cancer through up-regulating PTEN expression. Oncotarget. 7:66989–67003. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Zheng L, Jiao W, Mei H, Song H, Li D, Xiang X, Chen Y, Yang F, Li H, Huang K, et al: miRNA-337-3p inhibits gastric cancer progression through repressing myeloid zinc finger 1-facilitated expression of matrix metalloproteinase 14. Oncotarget. 7:40314–40328. 2016. View Article : Google Scholar : PubMed/NCBI

10 

Zhang PF, Sheng LL, Wang G, Tian M, Zhu LY, Zhang R, Zhang J and Zhu JS: miR-363 promotes proliferation and chemo-resistance of human gastric cancer via targeting of FBW7 ubiquitin ligase expression. Oncotarget. 7:35284–35292. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Bartels CL and Tsongalis GJ: MicroRNAs: Novel biomarkers for human cancer. Clin Chem. 55:623–631. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Khan S, Brougham CL, Ryan J, Sahrudin A, O'Neill G, Wall D, Curran C, Newell J, Kerin MJ and Dwyer RM: miR-379 regulates cyclin B1 expression and is decreased in breast cancer. PLoS One. 8:e687532013. View Article : Google Scholar : PubMed/NCBI

13 

Gururajan M, Josson S, Chu GC, Lu CL, Lu YT, Haga CL, Zhau HE, Liu C, Lichterman J, Duan P, et al: miR-154* and miR-379 in the DLK1-DIO3 microRNA mega-cluster regulate epithelial to mesenchymal transition and bone metastasis of prostate cancer. Clin Cancer Res. 20:6559–6569. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Pollari S, Leivonen SK, Perälä M, Fey V, Käkönen SM and Kallioniemi O: Identification of microRNAs inhibiting TGF-β-induced IL-11 production in bone metastatic breast cancer cells. PLoS One. 7:e373612012. View Article : Google Scholar

15 

Skalsky RL and Cullen BR: Reduced expression of brain-enriched microRNAs in glioblastomas permits targeted regulation of a cell death gene. PLoS One. 6:e242482011. View Article : Google Scholar : PubMed/NCBI

16 

Chen JS, Li HS, Huang JQ, Dong SH, Huang ZJ, Yi W, Zhan GF, Feng JT, Sun JC and Huang XH: MicroRNA-379-5p inhibits tumor invasion and metastasis by targeting FAK/AKT signaling in hepatocellular carcinoma. Cancer Lett. 375:73–83. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Yamamoto K, Seike M, Takeuchi S, Soeno C, Miyanaga A, Noro R, Minegishi Y, Kubota K and Gemma A: MiR-379/411 cluster regulates IL-18 and contributes to drug resistance in malignant pleural mesothelioma. Oncol Rep. 32:2365–2372. 2014.PubMed/NCBI

18 

Ge YZ, Xu LW, Xu Z, Wu R, Xin H, Zhu M, Lu TZ, Geng LG, Liu H, Zhou CC, et al: Expression profiles and clinical significance of MicroRNAs in papillary renal cell carcinoma: A STROBE-Compliant Observational Study. Medicine (Baltimore). 94:e7672015. View Article : Google Scholar

19 

Thiery JP, Acloque H, Huang RY and Nieto MA: Epithelial-mesenchymal transitions in development and disease. Cell. 139:871–890. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Zheng H and Kang Y: Multilayer control of the EMT master regulators. Oncogene. 33:1755–1763. 2014. View Article : Google Scholar

21 

Duan F, Jia D, Zhao J, Wu W, Min L, Song S, Wu H, Wang L, Wang H, Ruan Y, et al: Loss of GFAT1 promotes epithelial-to-mesenchymal transition and predicts unfavorable prognosis in gastric cancer. Oncotarget. 7:38427–38439. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Lee J, Ha S, Jung CK and Lee HH: High-mobility-group A2 overexpression provokes a poor prognosis of gastric cancer through the epithelial-mesenchymal transition. Int J Oncol. 46:2431–2438. 2015.PubMed/NCBI

23 

Yan Y, Zhang J, Li JH, Liu X, Wang JZ, Qu HY, Wang JS and Duan XY: High tumor-associated macrophages infiltration is associated with poor prognosis and may contribute to the phenomenon of epithelial-mesenchymal transition in gastric cancer. Onco Targets Ther. 9:3975–3983. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Hu J, Shan Z, Hu K, Ren F, Zhang W, Han M, Li Y, Feng K, Lei L and Feng Y: miRNA-223 inhibits epithelial-mesenchymal transition in gastric carcinoma cells via Sp1. Int J Oncol. 49:325–335. 2016.PubMed/NCBI

25 

Wang LL, Zhang XH, Zhang X and Chu JK: MiR-30a increases cisplatin sensitivity of gastric cancer cells through suppressing epithelial-to-mesenchymal transition (EMT). Eur Rev Med Pharmacol Sci. 20:1733–1739. 2016.PubMed/NCBI

26 

Zhang LL, Liu J, Lei S, Zhang J, Zhou W and Yu HG: PTEN inhibits the invasion and metastasis of gastric cancer via downregulation of FAK expression. Cell Signal. 26:1011–1020. 2014. View Article : Google Scholar : PubMed/NCBI

27 

Zhang PF, Li KS, Shen YH, Gao PT, Dong ZR, Cai JB, Zhang C, Huang XY, Tian MX, Hu ZQ, et al: Galectin-1 induces hepatocellular carcinoma EMT and sorafenib resistance by activating FAK/PI3K/AKT signaling. Cell Death Dis. 7:e22012016. View Article : Google Scholar : PubMed/NCBI

28 

Feng R and Yang S: Effects of combining erlotinib and RNA-interfered downregulation of focal adhesion kinase expression on gastric cancer. J Int Med Res. 44:855–864. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Chen P, Zhao H, Huang J, Yan X, Zhang Y and Gao Y: MicroRNA-17-5p promotes gastric cancer proliferation, migration and invasion by directly targeting early growth response 2. Am J Cancer Res. 6:2010–2020. 2016.PubMed/NCBI

30 

Sun J, Li J, Zhang W, Zhang J, Sun S, Li G, Song H and Wan D: MicroRNA-509-3p inhibits cancer cell proliferation and migration via upregulation of XIAP in gastric cancer cells. Oncol Res. 25:455–461. 2016. View Article : Google Scholar : PubMed/NCBI

31 

Park JH, Lee BL, Yoon J, Kim J, Kim MA, Yang HK and Kim WH: Focal adhesion kinase (FAK) gene amplification and its clinical implications in gastric cancer. Hum Pathol. 41:1664–1673. 2010. View Article : Google Scholar : PubMed/NCBI

32 

Zhou Y, Dang J, Chang KY, Yau E, Aza-Blanc P, Moscat J and Rana TM: miR-1298 inhibits mutant KRAS-driven tumor growth by repressing FAK and LAMB3. Cancer Res. 76:5777–5787. 2016. View Article : Google Scholar : PubMed/NCBI

33 

Guo LL, He ZC, Yang CQ, Qiao PT and Yin GL: Epigenetic silencing of olfactomedin-4 enhances gastric cancer cell invasion via activation of focal adhesion kinase signaling. BMB Rep. 48:630–635. 2015. View Article : Google Scholar : PubMed/NCBI

34 

Song G, Xu S, Zhang H, Wang Y, Xiao C, Jiang T, Wu L, Zhang T, Sun X, Zhong L, et al: TIMP1 is a prognostic marker for the progression and metastasis of colon cancer through FAK-PI3K/AKT and MAPK pathway. J Exp Clin Cancer Res. 35:1482016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xu M, Qin S, Cao F, Ding S and Li M: MicroRNA-379 inhibits metastasis and epithelial-mesenchymal transition via targeting FAK/AKT signaling in gastric cancer. Int J Oncol 51: 867-876, 2017.
APA
Xu, M., Qin, S., Cao, F., Ding, S., & Li, M. (2017). MicroRNA-379 inhibits metastasis and epithelial-mesenchymal transition via targeting FAK/AKT signaling in gastric cancer. International Journal of Oncology, 51, 867-876. https://doi.org/10.3892/ijo.2017.4072
MLA
Xu, M., Qin, S., Cao, F., Ding, S., Li, M."MicroRNA-379 inhibits metastasis and epithelial-mesenchymal transition via targeting FAK/AKT signaling in gastric cancer". International Journal of Oncology 51.3 (2017): 867-876.
Chicago
Xu, M., Qin, S., Cao, F., Ding, S., Li, M."MicroRNA-379 inhibits metastasis and epithelial-mesenchymal transition via targeting FAK/AKT signaling in gastric cancer". International Journal of Oncology 51, no. 3 (2017): 867-876. https://doi.org/10.3892/ijo.2017.4072
Copy and paste a formatted citation
x
Spandidos Publications style
Xu M, Qin S, Cao F, Ding S and Li M: MicroRNA-379 inhibits metastasis and epithelial-mesenchymal transition via targeting FAK/AKT signaling in gastric cancer. Int J Oncol 51: 867-876, 2017.
APA
Xu, M., Qin, S., Cao, F., Ding, S., & Li, M. (2017). MicroRNA-379 inhibits metastasis and epithelial-mesenchymal transition via targeting FAK/AKT signaling in gastric cancer. International Journal of Oncology, 51, 867-876. https://doi.org/10.3892/ijo.2017.4072
MLA
Xu, M., Qin, S., Cao, F., Ding, S., Li, M."MicroRNA-379 inhibits metastasis and epithelial-mesenchymal transition via targeting FAK/AKT signaling in gastric cancer". International Journal of Oncology 51.3 (2017): 867-876.
Chicago
Xu, M., Qin, S., Cao, F., Ding, S., Li, M."MicroRNA-379 inhibits metastasis and epithelial-mesenchymal transition via targeting FAK/AKT signaling in gastric cancer". International Journal of Oncology 51, no. 3 (2017): 867-876. https://doi.org/10.3892/ijo.2017.4072
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