Nicotine induces TIPE2 upregulation and Stat3 phosphorylation contributes to cholinergic anti-inflammatory effect

  • Authors:
    • Hua Xiu Sui
    • Shi Zhong Ke
    • Dan Dan Xu
    • Nan Nan Lu
    • Yi Nan Wang
    • Yue Hua Zhang
    • Feng Guang Gao
  • View Affiliations

  • Published online on: July 27, 2017     https://doi.org/10.3892/ijo.2017.4080
  • Pages: 987-995
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Abstract

Cholinergic anti-inflammatory pathway has therapeutic effect on inflammation-associated diseases. However, the exact mechanism of nicotine-mediated anti-inflammatory effect is still unclear. TIPE2, a new member of tumor necrosis factor-α-induced protein-8 family, is a negative regulator of immune homeostasis. However, the roles of TIPE2 in cholinergic anti-inflammatory effect are still uncertain. Here, we demonstrated that nicotine exerts its anti-inflammatory effect by TIPE2 upregulation and phosphorylated stat3 mediated the inhibition of NF-κB activation, which was supported by the following evidence: firstly, both nicotine and TIPE2 inhibit pro-inflammatory cytokine release via NF-κB inactivation. Secondly, nicotine upregulates TIPE2 expression via α7 nicotinic acetylcholine receptor. Moreover, the enhancement of stat3 phosphorylation and decrease of LPS-induced p65 translocation were achieved by nicotine treatment. Importantly, nicotine treatment augments the interaction of phosphorylated stat3 and p65, indicating that the inhibitory effect of nicotine on NF-κB activation was mediated with protein-protein interactions. Hence, this study revealed that TIPE2 upregulation and stat3 phosphorylation contribute to nicotine-mediated anti-inflammation effect, indicating that TIPE2 and stat3 might be potential molecules for dealing with inflammation-associated diseases.
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September 2017
Volume 51 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Sui, H., Ke, S., Xu, D., Lu, N., Wang, Y., Zhang, Y., & Gao, F. (2017). Nicotine induces TIPE2 upregulation and Stat3 phosphorylation contributes to cholinergic anti-inflammatory effect. International Journal of Oncology, 51, 987-995. https://doi.org/10.3892/ijo.2017.4080
MLA
Sui, H., Ke, S., Xu, D., Lu, N., Wang, Y., Zhang, Y., Gao, F."Nicotine induces TIPE2 upregulation and Stat3 phosphorylation contributes to cholinergic anti-inflammatory effect". International Journal of Oncology 51.3 (2017): 987-995.
Chicago
Sui, H., Ke, S., Xu, D., Lu, N., Wang, Y., Zhang, Y., Gao, F."Nicotine induces TIPE2 upregulation and Stat3 phosphorylation contributes to cholinergic anti-inflammatory effect". International Journal of Oncology 51, no. 3 (2017): 987-995. https://doi.org/10.3892/ijo.2017.4080