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Human amniotic epithelial cells inhibit growth of epithelial ovarian cancer cells via TGF‑β1-mediated cell cycle arrest

  • Authors:
    • Shixia Bu
    • Qiuwan Zhang
    • Qian Wang
    • Dongmei Lai
  • View Affiliations / Copyright

    Affiliations: The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200030, P.R. China
    Copyright: © Bu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1405-1414
    |
    Published online on: September 14, 2017
       https://doi.org/10.3892/ijo.2017.4123
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Abstract

It is reported that human amniotic epithelial cells (hAECs) endow intrinsic antitumor effects on certain kinds of cancer. This research was designed to evaluate whether hAECs endowed potential anticancer properties on epithelial ovarian cancer (EOC) cells in vivo and in vitro, which has not been reported before. In this study, we established a xenografted BALB/c nude mouse model by subcutaneously co-injecting ovarian cancer cell line, SK-OV-3, and hAECs for 28 days. In ex vivo experiments, CCK‑8 cell viability assay, real-time PCR, cell counting assay, cell cycle analysis and immunohistochemistry (IHC) assay were used to detect the effects of hAEC‑secreted factors on the proliferation and cell cycle progression of EOC cells. A cytokine array was conducted to detect anticancer-related cytokines released from hAECs. Human recombinant TGF‑β1 and TGF‑β1 antibody were used to treat EOC cells and analyzed whether TGF‑β1 contributed to the cell cycle arrest. Results from in vivo and ex vivo experiments showed that hAEC-secreted factors and rhTGF‑β1 decreased proliferation of EOC cells and induced G0/G1 cell cycle arrest in cancer cells, which could be partially reversed by excess TGF‑β1 antibody. These data indicate that hAECs endow potential anticancer properties on epithelial ovarian cancer in vivo and in vitro which is partially mediated by hAEC‑secreted TGF‑β1-induced cell cycle arrest. This study suggests a potential application of hAEC‑based therapy against epithelial ovarian cancer.
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1 

Barbati A, Grazia Mameli M, Sidoni A and Di Renzo GC: Amniotic membrane: Separation of amniotic mesoderm from amniotic epithelium and isolation of their respective mesenchymal stromal and epithelial cells. Curr Protoc Stem Cell Biol Chapter. 1:82012.

2 

Ilancheran S, Michalska A, Peh G, Wallace EM, Pera M and Manuelpillai U: Stem cells derived from human fetal membranes display multilineage differentiation potential. Biol Reprod. 77:577–588. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Miki T, Lehmann T, Cai H, Stolz DB and Strom SC: Stem cell characteristics of amniotic epithelial cells. Stem Cells. 23:1549–1559. 2005. View Article : Google Scholar : PubMed/NCBI

4 

Yao X, Guo Y, Wang Q, Xu M, Zhang Q, Li T and Lai D: The paracrine effect of transplanted human amniotic epithelial cells on ovarian function improvement in a mouse model of chemotherapy-induced primary ovarian insufficiency. Stem Cells Int. 2016:41489232016. View Article : Google Scholar

5 

Si JW, Zhang JJ, Dai JW, Yu DD, Yu HB, Shi J, Wang XD, Shen SGF and Guo LH: Osteogenic differentiation of human amniotic epithelial cells and its application in alveolar defect restoration. Stem Cells Transl Med. 3:1504–1513. 2014. View Article : Google Scholar

6 

Jiang LW, Chen H and Lu H: Using human epithelial amnion cells in human de-epidermized dermis for skin regeneration. J Dermatol Sci. 81:26–34. 2016. View Article : Google Scholar

7 

Niknejad H, Paeini-Vayghan G, Tehrani FA, Khayat-Khoei M and Peirovi H: Side dependent effects of the human amnion on angiogenesis. Placenta. 34:340–345. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Niknejad H, Khayat-Khoei M, Peirovi H and Abolghasemi H: Human amniotic epithelial cells induce apoptosis of cancer cells: A new anti-tumor therapeutic strategy. Cytotherapy. 16:33–40. 2014. View Article : Google Scholar

9 

Kang NH, Yi BR, Lim SY, Hwang KA, Baek YS, Kang KS and Choi KC: Human amniotic membrane-derived epithelial stem cells display anticancer activity in BALB/c female nude mice bearing disseminated breast cancer xenografts. Int J Oncol. 40:2022–2028. 2012.PubMed/NCBI

10 

Mamede AC, Laranjo M, Carvalho MJ, Abrantes AM, Pires AS, Brito AF, Moura P, Maia CJ and Botelho MF: Effect of amniotic membrane proteins in human cancer cell lines: An exploratory study. J Membr Biol. 247:357–360. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Zhang Q, Xu M, Yao X, Li T, Wang Q and Lai D: Human amniotic epithelial cells inhibit granulosa cell apoptosis induced by chemotherapy and restore the fertility. Stem Cell Res Ther. 6:1522015. View Article : Google Scholar : PubMed/NCBI

12 

Liu T, Zhao L, Zhang Y, Chen W, Liu D, Hou H, Ding L and Li X: Ginsenoside 20(S)-Rg3 targets HIF-1α to block hypoxia-induced epithelial-mesenchymal transition in ovarian cancer cells. PLoS One. 9:e1038872014. View Article : Google Scholar

13 

Budwit-Novotny DA, McCarty KS, Cox EB, Soper JT, Mutch DG, Creasman WT, Flowers JL and McCarty KS Jr: Immunohistochemical analyses of estrogen receptor in endometrial adenocarcinoma using a monoclonal antibody. Cancer Res. 46:5419–5425. 1986.PubMed/NCBI

14 

Jin G, Cao Z, Sun X, Wang K, Huang T and Shen B: Protein O-glucosyltransferase 1 overexpression downregulates p16 in BT474 human breast cancer cells. Oncol Lett. 8:594–600. 2014.PubMed/NCBI

15 

Datto MB, Yu Y and Wang XF: Functional analysis of the transforming growth factor beta responsive elements in the WAF1/Cip1/p21 promoter. J Biol Chem. 270:28623–28628. 1995. View Article : Google Scholar : PubMed/NCBI

16 

Kim GY, Mercer SE, Ewton DZ, Yan Z, Jin K and Friedman E: The stress-activated protein kinases p38 alpha and JNK1 stabilize p21 (Cip1) by phosphorylation. J Biol Chem. 277:29792–29802. 2002. View Article : Google Scholar : PubMed/NCBI

17 

Wang F, Wang L, Yao X, Lai D and Guo L: Human amniotic epithelial cells can differentiate into granulosa cells and restore folliculogenesis in a mouse model of chemotherapy-induced premature ovarian failure. Stem Cell Res Ther. 4:1242013. View Article : Google Scholar :

18 

Jayson GC, Kohn EC, Kitchener HC and Ledermann JA: Ovarian cancer. Lancet. 384:1376–1388. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Di Germanio C, Bernier M, Petr M, Mattioli M, Barboni B and de Cabo R: Conditioned medium derived from rat amniotic epithelial cells confers protection against inflammation, cancer, and senescence. Oncotarget. 7:39051–39064. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Dickson MA: Molecular pathways: CDK4 inhibitors for cancer therapy. Clin Cancer Res. 20:3379–3383. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Magatti M, De Munari S, Vertua E and Parolini O: Amniotic membrane-derived cells inhibit proliferation of cancer cell lines by inducing cell cycle arrest. J Cell Mol Med. 16:2208–2218. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Chen X, Zheng S, Gao Y, Dai H, Mou H and Yang H: Growth regulation of ovarian cancer cell line HO-8910 by transforming growth factor beta 1 in vitro. Chin Med J (Engl). 111:546–550. 1998.

23 

Ketley NJ, Allen PD, Kelsey SM and Newland AC: Modulation of idarubicin-induced apoptosis in human acute myeloid leukemia blasts by all-trans retinoic acid, 1,25(OH)2 vitamin D3, and granulocyte-macrophage colony-stimulating factor. Blood. 90:4578–4587. 1997.PubMed/NCBI

24 

Schinke C, Giricz O, Li W, Shastri A, Gordon S, Barreyro L, Bhagat T, Bhattacharyya S, Ramachandra N, Bartenstein M, et al: IL8-CXCR2 pathway inhibition as a therapeutic strategy against MDS and AML stem cells. Blood. 125:3144–3152. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Resnitzky D, Tiefenbrun N, Berissi H and Kimchi A: Interferons and interleukin 6 suppress phosphorylation of the retinoblastoma protein in growth-sensitive hematopoietic cells. Proc Natl Acad Sci USA. 89:402–406. 1992. View Article : Google Scholar : PubMed/NCBI

26 

Muthukkumar S, Sells SF, Crist SA and Rangnekar VM: Interleukin-1 induces growth arrest by hypophosphorylation of the retinoblastoma susceptibility gene product RB. J Biol Chem. 271:5733–5740. 1996. View Article : Google Scholar : PubMed/NCBI

27 

Lillibridge CD and O'Connell BC: In human salivary gland cells, overexpression of E2F1 overcomes an interferon-gamma- and tumor necrosis factor-alpha-induced growth arrest but does not result in complete mitosis. J Cell Physiol. 172:343–350. 1997. View Article : Google Scholar : PubMed/NCBI

28 

Wu DT, Bitzer M, Ju W, Mundel P and Böttinger EP: TGF-beta concentration specifies differential signaling profiles of growth arrest/differentiation and apoptosis in podocytes. J Am Soc Nephrol. 16:3211–3221. 2005. View Article : Google Scholar : PubMed/NCBI

29 

Kitamura K, Aota S, Sakamoto R, Emori T and Okazaki K: Smad7 induces G0/G1 cell cycle arrest in mesenchymal cells by inhibiting the expression of G1 cyclins. Dev Growth Differ. 47:537–552. 2005. View Article : Google Scholar : PubMed/NCBI

30 

Malumbres M and Barbacid M: Cell cycle, CDKs and cancer: A changing paradigm. Nat Rev Cancer. 9:153–166. 2009. View Article : Google Scholar : PubMed/NCBI

31 

Schlosshauer PW, Deligdisch L, Penault-Llorca F, Fatemi D, Qiao R, Yao S, Pearl M, Yang Z, Sheng T and Dong J: Loss of p16I NK4A expression in low-grade ovarian serous carcinomas. Int J Gynecol Pathol. 30:22–29. 2011. View Article : Google Scholar

32 

Kudoh K, Ichikawa Y, Yoshida S, Hirai M, Kikuchi Y, Nagata I, Miwa M and Uchida K: Inactivation of p16/CDKN2 and p15/MTS2 is associated with prognosis and response to chemotherapy in ovarian cancer. Int J Cancer. 99:579–582. 2002. View Article : Google Scholar : PubMed/NCBI

33 

Buchynska LG, Nesina IP, Yurchenko NP, Bilyk OO, Grinkevych VN and Svintitsky VS: Expression of p53, p21WAF1/CIP1, p16INK4A and Ki-67 proteins in serous ovarian tumors. Exp Oncol. 29:49–53. 2007.PubMed/NCBI

34 

Bali A, O'Brien PM, Edwards LS, Sutherland RL, Hacker NF and Henshall SM: Cyclin D1, p53, and p21Waf1/Cip1 expression is predictive of poor clinical outcome in serous epithelial ovarian cancer. Clin Cancer Res. 10:5168–5177. 2004. View Article : Google Scholar : PubMed/NCBI

35 

Khalil N, Xu YD, O'Connor R and Duronio V: Proliferation of pulmonary interstitial fibroblasts is mediated by transforming growth factor-beta1-induced release of extracellular fibroblast growth factor-2 and phosphorylation of p38 MAPK and JNK. J Biol Chem. 280:43000–43009. 2005. View Article : Google Scholar : PubMed/NCBI

36 

Henley SA and Dick FA: The retinoblastoma family of proteins and their regulatory functions in the mammalian cell division cycle. Cell Div. 7:102012. View Article : Google Scholar : PubMed/NCBI

37 

Francis SM, Bergsied J, Isaac CE, Coschi CH, Martens AL, Hojilla CV, Chakrabarti S, Dimattia GE, Khoka R, Wang JY, et al: A functional connection between pRB and transforming growth factor beta in growth inhibition and mammary gland development. Mol Cell Biol. 29:4455–4466. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Bu S, Zhang Q, Wang Q and Lai D: Human amniotic epithelial cells inhibit growth of epithelial ovarian cancer cells via TGF‑β1-mediated cell cycle arrest. Int J Oncol 51: 1405-1414, 2017.
APA
Bu, S., Zhang, Q., Wang, Q., & Lai, D. (2017). Human amniotic epithelial cells inhibit growth of epithelial ovarian cancer cells via TGF‑β1-mediated cell cycle arrest. International Journal of Oncology, 51, 1405-1414. https://doi.org/10.3892/ijo.2017.4123
MLA
Bu, S., Zhang, Q., Wang, Q., Lai, D."Human amniotic epithelial cells inhibit growth of epithelial ovarian cancer cells via TGF‑β1-mediated cell cycle arrest". International Journal of Oncology 51.5 (2017): 1405-1414.
Chicago
Bu, S., Zhang, Q., Wang, Q., Lai, D."Human amniotic epithelial cells inhibit growth of epithelial ovarian cancer cells via TGF‑β1-mediated cell cycle arrest". International Journal of Oncology 51, no. 5 (2017): 1405-1414. https://doi.org/10.3892/ijo.2017.4123
Copy and paste a formatted citation
x
Spandidos Publications style
Bu S, Zhang Q, Wang Q and Lai D: Human amniotic epithelial cells inhibit growth of epithelial ovarian cancer cells via TGF‑β1-mediated cell cycle arrest. Int J Oncol 51: 1405-1414, 2017.
APA
Bu, S., Zhang, Q., Wang, Q., & Lai, D. (2017). Human amniotic epithelial cells inhibit growth of epithelial ovarian cancer cells via TGF‑β1-mediated cell cycle arrest. International Journal of Oncology, 51, 1405-1414. https://doi.org/10.3892/ijo.2017.4123
MLA
Bu, S., Zhang, Q., Wang, Q., Lai, D."Human amniotic epithelial cells inhibit growth of epithelial ovarian cancer cells via TGF‑β1-mediated cell cycle arrest". International Journal of Oncology 51.5 (2017): 1405-1414.
Chicago
Bu, S., Zhang, Q., Wang, Q., Lai, D."Human amniotic epithelial cells inhibit growth of epithelial ovarian cancer cells via TGF‑β1-mediated cell cycle arrest". International Journal of Oncology 51, no. 5 (2017): 1405-1414. https://doi.org/10.3892/ijo.2017.4123
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