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Brain lipid-binding protein promotes proliferation and modulates cell cycle in C6 rat glioma cells

  • Authors:
    • Xiao Han
    • Haoming Li
    • Ye Zhang
    • Jianbing Qin
    • Qingqing Yang
    • Lu Wang
    • Mingjie Yuan
    • Chunlin Xia
  • View Affiliations / Copyright

    Affiliations: Department of Anatomy and Cytoneurobiology Unit, Medical College of Soochow University, Suzhou, Jiangsu 215123, P.R. China, Department of Human Anatomy, Medical School of Nantong University, Nantong, Jiangsu 226001, P.R. China, Department of Medicine, Xinglin College of Nantong University, Nantong, Jiangsu 226001, P.R. China
    Copyright: © Han et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1439-1448
    |
    Published online on: September 22, 2017
       https://doi.org/10.3892/ijo.2017.4132
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Abstract

Gliomas are the most common primary brain tumors affecting adults. Four grades of gliomas have been identified, namely, grades I-IV. Brain lipid-binding protein (BLBP), which functions in the intracellular transport of fatty acids, is expressed in all grades of human gliomas. The glioma cells that are cultured in vitro are grouped into the BLBP-positive and BLBP-negative cell lines. In the present study, we found that C6 rat glioma cells was a distinct type of BLBP-negative cell line. Our results confirmed that in the C6 cells, the expression of exogenous BLBP increased the proliferation and percentage of cells in the S phase, in the culture medium containing 10 or 1% FBS. Moreover, exogenous BLBP was found to downregulate the tumor suppressors p21 and p16 in the 1% FBS culture medium, but only p21 in the 10% FBS culture medium. The results of the xenograft model assay showed that exogenous BLBP also stimulated tumor formation and downregulated p21 and p16. In conclusion, our study demonstrated that exogenous BLBP promoted proliferation of the C6 cells in vitro and facilitated tumor formation in vivo. Therefore, BLBP expression in glioma cells may promote cell growth by inhibiting the tumor suppressors.
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Copy and paste a formatted citation
Spandidos Publications style
Han X, Li H, Zhang Y, Qin J, Yang Q, Wang L, Yuan M and Xia C: Brain lipid-binding protein promotes proliferation and modulates cell cycle in C6 rat glioma cells. Int J Oncol 51: 1439-1448, 2017.
APA
Han, X., Li, H., Zhang, Y., Qin, J., Yang, Q., Wang, L. ... Xia, C. (2017). Brain lipid-binding protein promotes proliferation and modulates cell cycle in C6 rat glioma cells. International Journal of Oncology, 51, 1439-1448. https://doi.org/10.3892/ijo.2017.4132
MLA
Han, X., Li, H., Zhang, Y., Qin, J., Yang, Q., Wang, L., Yuan, M., Xia, C."Brain lipid-binding protein promotes proliferation and modulates cell cycle in C6 rat glioma cells". International Journal of Oncology 51.5 (2017): 1439-1448.
Chicago
Han, X., Li, H., Zhang, Y., Qin, J., Yang, Q., Wang, L., Yuan, M., Xia, C."Brain lipid-binding protein promotes proliferation and modulates cell cycle in C6 rat glioma cells". International Journal of Oncology 51, no. 5 (2017): 1439-1448. https://doi.org/10.3892/ijo.2017.4132
Copy and paste a formatted citation
x
Spandidos Publications style
Han X, Li H, Zhang Y, Qin J, Yang Q, Wang L, Yuan M and Xia C: Brain lipid-binding protein promotes proliferation and modulates cell cycle in C6 rat glioma cells. Int J Oncol 51: 1439-1448, 2017.
APA
Han, X., Li, H., Zhang, Y., Qin, J., Yang, Q., Wang, L. ... Xia, C. (2017). Brain lipid-binding protein promotes proliferation and modulates cell cycle in C6 rat glioma cells. International Journal of Oncology, 51, 1439-1448. https://doi.org/10.3892/ijo.2017.4132
MLA
Han, X., Li, H., Zhang, Y., Qin, J., Yang, Q., Wang, L., Yuan, M., Xia, C."Brain lipid-binding protein promotes proliferation and modulates cell cycle in C6 rat glioma cells". International Journal of Oncology 51.5 (2017): 1439-1448.
Chicago
Han, X., Li, H., Zhang, Y., Qin, J., Yang, Q., Wang, L., Yuan, M., Xia, C."Brain lipid-binding protein promotes proliferation and modulates cell cycle in C6 rat glioma cells". International Journal of Oncology 51, no. 5 (2017): 1439-1448. https://doi.org/10.3892/ijo.2017.4132
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