miR-768-3p is involved in the proliferation, invasion and migration of non-small cell lung carcinomas

Xie,Zuozhou; Chen,Weiqiang; Chen,Yinghua; Wang,Xiang; Gao,Wenyong; Liu,Yi

doi:10.3892/ijo.2017.4133

miR-768-3p is involved in the proliferation, invasion and migration of non-small cell lung carcinomas

Authors:
- Zuozhou Xie
- Weiqiang Chen
- Yinghua Chen
- Xiang Wang
- Wenyong Gao
- Yi Liu
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Affiliations: Department of Respiratory and Critical Medicine, No. 2 People's Hospital of Kunming, Kunming, Yunnan 650204, P.R. China
Published online on: September 22, 2017 https://doi.org/10.3892/ijo.2017.4133
Pages: 1574-1582

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Abstract

Altered microRNA expression has been found to be a common feature of several cancers, including lung carcinomas. However, the possible roles of miR-768-3p in the pathological changes of lung carcinomas are still unknown. The aim of the present study was to investigate the expression and possible effects of miR-768-3p in human non-small cell lung carcinomas (NSCLC). Eighty-three NSCLC patients attending the clinic of Kunming Hospital were invited to participate in the study. Their tumor samples were obtained for qRT-PCR analysis. Human NSCLC cell lines, A549 and HCC4006, were employed and transfected with either miR-768-3p mimics or miR-768-3p antagomir. Following transfection, the in vitro and in vivo proliferation, apoptosis fractions, migration and invasion of NSCLC cells were evaluated. The data revealed that: i) upregulated miR-768-3p in tumors were associated with the clinicopathological features of NSCLC patients; ii) inhibiting miR-768-3p function by miR-768-3p antagomir induced distinctly apoptosis and Fas/FasL expressional alteration of NSCLC cells; iii) miR-768-3p antagomir transduction also decreased the viability, migration and invasion, as well as MMP-2 and MMP-9 activities in A549 and HCC4006 cells; and iv) miR-768-3p antagomir transfection also inhibited the growth and proliferation of NSCLC xenografts in nude mice. The present results suggested that abnormal elevated miR-768-3p in NSCLC tumors and cell lines played important roles in NSCLC carcinogenic progression, and the targeting of miR-768-3p might be a potential therapeutic strategy for the treatment of NSCLC.

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