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Article Open Access

Adoptive immunotherapy combined with FP treatment for head and neck cancer: An in vitro study

  • Authors:
    • Mayako Nishio-Nagai
    • Susumu Suzuki
    • Kazuhiro Yoshikawa
    • Ryuzo Ueda
    • Yoshiaki Kazaoka
  • View Affiliations / Copyright

    Affiliations: Department of Oral and Maxillofacial Surgery, Aichi Medical University, Nagakute, Aichi 480-1195, Japan, Department of Tumor Immunology, Aichi Medical University School of Medicine, Nagakute, Aichi 480-1195, Japan, Division of Advanced Research Promotion, Institute of Comprehensive Medical Research, Aichi Medical University School of Medicine, Nagakute, Aichi 480-1195, Japan
    Copyright: © Nishio-Nagai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1471-1481
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    Published online on: October 2, 2017
       https://doi.org/10.3892/ijo.2017.4142
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Abstract

FP treatment, which combines 5-fluorouracil (5-FU) and cisplatin (CDDP) chemotherapy, is widely used for treatment of advanced head and neck cancer (HNC). It has been suggested that these drugs cause immunomodulation in the cancer microenvironment, for example, downregulation of immunosuppressive cells such as regulatory T-cells (Tregs) and myeloid-derived suppressive cells (MDSCs), activating dendritic cells (DCs), and upregulation of tumor antigens and major histocompatibility complex (MHC) molecules in cancer cells leads to enhancement of cancer immunity, which is important in cancer treatment, as well as providing a direct killing effect. Therefore, development of chemoimmunotherapy by combining FP treatment with immunotherapy for HNC has become a recent challenging issue. However, the direct effects of these drugs on immune effector cells, especially cytotoxic T-lymphocytes (CTLs), are not well known. We have investigated the direct actions of these drugs on CTL functions in in vitro experiments using cytomegalovirus (CMV) pp65 antigen-specific CTLs (CMVpp65-CTLs) and oral squamous cell cancer (OSCC) cell lines overexpressing CMVpp65 antigen as target cells. Although CDDP partially inhibited proliferation of memory CMVpp65-CTL in peripheral blood, the proliferation was not inhibited by 5-FU. Cytotoxicity and the IFN-γ release response of the CMVpp65-CTLs were not inhibited by these drugs, and it is important to note that these drugs, especially 5-FU, sensitized OSCC cell lines to CMVpp65-CTL. Furthermore, CMVpp65-CTL cytotoxicity to CDDP-resistant OSCC cells, HSC-3/CDDP-R1, was the same as the cytotoxicity to the parental cells. Thus, we suggest that combined immunotherapy with FP treatment is an effective novel HNC treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Nishio-Nagai M, Suzuki S, Yoshikawa K, Ueda R and Kazaoka Y: Adoptive immunotherapy combined with FP treatment for head and neck cancer: An in vitro study. Int J Oncol 51: 1471-1481, 2017.
APA
Nishio-Nagai, M., Suzuki, S., Yoshikawa, K., Ueda, R., & Kazaoka, Y. (2017). Adoptive immunotherapy combined with FP treatment for head and neck cancer: An in vitro study. International Journal of Oncology, 51, 1471-1481. https://doi.org/10.3892/ijo.2017.4142
MLA
Nishio-Nagai, M., Suzuki, S., Yoshikawa, K., Ueda, R., Kazaoka, Y."Adoptive immunotherapy combined with FP treatment for head and neck cancer: An in vitro study". International Journal of Oncology 51.5 (2017): 1471-1481.
Chicago
Nishio-Nagai, M., Suzuki, S., Yoshikawa, K., Ueda, R., Kazaoka, Y."Adoptive immunotherapy combined with FP treatment for head and neck cancer: An in vitro study". International Journal of Oncology 51, no. 5 (2017): 1471-1481. https://doi.org/10.3892/ijo.2017.4142
Copy and paste a formatted citation
x
Spandidos Publications style
Nishio-Nagai M, Suzuki S, Yoshikawa K, Ueda R and Kazaoka Y: Adoptive immunotherapy combined with FP treatment for head and neck cancer: An in vitro study. Int J Oncol 51: 1471-1481, 2017.
APA
Nishio-Nagai, M., Suzuki, S., Yoshikawa, K., Ueda, R., & Kazaoka, Y. (2017). Adoptive immunotherapy combined with FP treatment for head and neck cancer: An in vitro study. International Journal of Oncology, 51, 1471-1481. https://doi.org/10.3892/ijo.2017.4142
MLA
Nishio-Nagai, M., Suzuki, S., Yoshikawa, K., Ueda, R., Kazaoka, Y."Adoptive immunotherapy combined with FP treatment for head and neck cancer: An in vitro study". International Journal of Oncology 51.5 (2017): 1471-1481.
Chicago
Nishio-Nagai, M., Suzuki, S., Yoshikawa, K., Ueda, R., Kazaoka, Y."Adoptive immunotherapy combined with FP treatment for head and neck cancer: An in vitro study". International Journal of Oncology 51, no. 5 (2017): 1471-1481. https://doi.org/10.3892/ijo.2017.4142
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