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Suppression of tumor-derived Semaphorin 7A and genetic ablation of host-derived Semaphorin 7A impairs tumor progression in a murine model of advanced breast carcinoma

  • Authors:
    • R. Garcia-Areas
    • S. Libreros
    • M. Simoes
    • C. Castro-Silva
    • N. Gazaniga
    • S. Amat
    • J. Jaczewska
    • P. Keating
    • K. Schilling
    • M. Brito
    • E. P. Wojcikiewicz
    • V. Iragavarpu-Charyulu
  • View Affiliations / Copyright

    Affiliations: Department of Biomedical Sciences, Charles E. Schmidt College of Medicine, Florida Atlantic University, Boca Raton, FL 33431, USA, Lynn Women's Health & Wellness Institute, Boca Raton Regional Hospital, Boca Raton, FL 33431, USA, Department of Pathology, Boca Raton Regional Hospital, Boca Raton, FL 33431, USA
    Copyright: © Garcia-Areas et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1395-1404
    |
    Published online on: October 3, 2017
       https://doi.org/10.3892/ijo.2017.4144
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Abstract

Solid tumors can generate a plethora of neurogenesis-related molecules that enhance their growth and metastasis. Among them, we have identified axonal guidance molecule Semaphorin 7A (SEMA7A) in breast cancer. The goal of this study was to determine the therapeutic effect of suppressing SEMA7A levels in the 4T1 murine model of advanced breast carcinoma. We used anti-SEMA7A short hairpin RNA (shRNA) to gene silence SEMA7A in 4T1 mammary tumor cells. When implanted into the mammary fat pads of syngeneic mice, SEMA7A shRNA-expressing 4T1 tumors exhibited decreased growth rates, deferred metastasis and reduced mortality. In vitro, SEMA7A shRNA-expressing 4T1 cells had weakened proliferative, migratory and invasive abilities, and decreased levels of mesenchymal factors. Atomic force microscopy studies showed that SEMA7A shRNA-expressing 4T1 cells had an increase in cell stiffness that corresponded with their decreased malignant potential. Genetic ablation of host-derived SEMA7A further enhanced the antitumor effects of SEMA7A shRNA gene silencing in 4T1 cells. Our preclinical findings demonstrate a critical role for SEMA7A in mediating mammary tumor progression.
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Copy and paste a formatted citation
Spandidos Publications style
Garcia-Areas R, Libreros S, Simoes M, Castro-Silva C, Gazaniga N, Amat S, Jaczewska J, Keating P, Schilling K, Brito M, Brito M, et al: Suppression of tumor-derived Semaphorin 7A and genetic ablation of host-derived Semaphorin 7A impairs tumor progression in a murine model of advanced breast carcinoma. Int J Oncol 51: 1395-1404, 2017.
APA
Garcia-Areas, R., Libreros, S., Simoes, M., Castro-Silva, C., Gazaniga, N., Amat, S. ... Iragavarpu-Charyulu, V. (2017). Suppression of tumor-derived Semaphorin 7A and genetic ablation of host-derived Semaphorin 7A impairs tumor progression in a murine model of advanced breast carcinoma. International Journal of Oncology, 51, 1395-1404. https://doi.org/10.3892/ijo.2017.4144
MLA
Garcia-Areas, R., Libreros, S., Simoes, M., Castro-Silva, C., Gazaniga, N., Amat, S., Jaczewska, J., Keating, P., Schilling, K., Brito, M., Wojcikiewicz, E. P., Iragavarpu-Charyulu, V."Suppression of tumor-derived Semaphorin 7A and genetic ablation of host-derived Semaphorin 7A impairs tumor progression in a murine model of advanced breast carcinoma". International Journal of Oncology 51.5 (2017): 1395-1404.
Chicago
Garcia-Areas, R., Libreros, S., Simoes, M., Castro-Silva, C., Gazaniga, N., Amat, S., Jaczewska, J., Keating, P., Schilling, K., Brito, M., Wojcikiewicz, E. P., Iragavarpu-Charyulu, V."Suppression of tumor-derived Semaphorin 7A and genetic ablation of host-derived Semaphorin 7A impairs tumor progression in a murine model of advanced breast carcinoma". International Journal of Oncology 51, no. 5 (2017): 1395-1404. https://doi.org/10.3892/ijo.2017.4144
Copy and paste a formatted citation
x
Spandidos Publications style
Garcia-Areas R, Libreros S, Simoes M, Castro-Silva C, Gazaniga N, Amat S, Jaczewska J, Keating P, Schilling K, Brito M, Brito M, et al: Suppression of tumor-derived Semaphorin 7A and genetic ablation of host-derived Semaphorin 7A impairs tumor progression in a murine model of advanced breast carcinoma. Int J Oncol 51: 1395-1404, 2017.
APA
Garcia-Areas, R., Libreros, S., Simoes, M., Castro-Silva, C., Gazaniga, N., Amat, S. ... Iragavarpu-Charyulu, V. (2017). Suppression of tumor-derived Semaphorin 7A and genetic ablation of host-derived Semaphorin 7A impairs tumor progression in a murine model of advanced breast carcinoma. International Journal of Oncology, 51, 1395-1404. https://doi.org/10.3892/ijo.2017.4144
MLA
Garcia-Areas, R., Libreros, S., Simoes, M., Castro-Silva, C., Gazaniga, N., Amat, S., Jaczewska, J., Keating, P., Schilling, K., Brito, M., Wojcikiewicz, E. P., Iragavarpu-Charyulu, V."Suppression of tumor-derived Semaphorin 7A and genetic ablation of host-derived Semaphorin 7A impairs tumor progression in a murine model of advanced breast carcinoma". International Journal of Oncology 51.5 (2017): 1395-1404.
Chicago
Garcia-Areas, R., Libreros, S., Simoes, M., Castro-Silva, C., Gazaniga, N., Amat, S., Jaczewska, J., Keating, P., Schilling, K., Brito, M., Wojcikiewicz, E. P., Iragavarpu-Charyulu, V."Suppression of tumor-derived Semaphorin 7A and genetic ablation of host-derived Semaphorin 7A impairs tumor progression in a murine model of advanced breast carcinoma". International Journal of Oncology 51, no. 5 (2017): 1395-1404. https://doi.org/10.3892/ijo.2017.4144
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