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Article

Blocking interleukin-6 signaling inhibits cell viability/proliferation, glycolysis, and colony forming activity of human medulloblastoma cells

  • Authors:
    • Xiang Chen
    • Jia Wei
    • Chenglong Li
    • Christopher R. Pierson
    • Jonathan L. Finlay
    • Jiayuh Lin
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry and Molecular Biology, School of Medicine, University of Maryland, Baltimore, MD 21201, USA, College of Pharmacy, University of Florida, Gainesville, FL 32610, USA, Department of Pathology and Laboratory Medicine, Nationwide Children's Hospital, The Department of Pathology and Division of Anatomy, College of Medicine, The Ohio State University, Columbus, OH 43205, USA, Hematology and Oncology, The Research Institute at Nationwide Children's Hospital, Department of Pediatrics, College of Medicine, The Ohio State University, Columbus, OH 43205, USA
  • Pages: 571-578
    |
    Published online on: November 22, 2017
       https://doi.org/10.3892/ijo.2017.4211
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Abstract

Elevated levels of the pro-inflammatory cytokine interleukin-6 (IL‑6) have tumor-promoting activity and are associated with poor survival outcomes in many cancers. Additionally, the IL‑6/GP130/STAT3 axis has been widely studied due to its pivotal role in tumor development and maintenance in a number of tissue types, including the cerebellum. However, the connection between IL‑6 signaling and medulloblastoma progression is largely unexplored. In the present study, we observed that IL‑6 induced medulloblastoma cell viability, cell proliferation and glycolysis. Furthermore, it also upregulated the expression of phosphorylated STAT3, indicating that the IL‑6/GP130/STAT3 pathway plays a central role in medulloblastoma. The FDA-approved drug bazedoxifene, a blocker of the formation of the hexameric IL‑6/IL‑6R/GP130 complex, was re-purposed in this study to inhibit the IL‑6/GP130/STAT3 signaling pathway. Bazedoxifene not only inhibited IL‑6 mediated cell viability and cell proliferation, and increased phosphorylated STAT3 expression, but it also decreased cell glycolysis, demonstrating a certain level of therapeutic efficacy in vitro. Collectively, our findings offer new insight into the molecular mechanism underlying the biological aggressiveness of medulloblastoma, the roles of IL‑6 in these processes and a possible efficacious adjuvant therapy for medulloblastoma.
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Copy and paste a formatted citation
Spandidos Publications style
Chen X, Wei J, Li C, Pierson CR, Finlay JL and Lin J: Blocking interleukin-6 signaling inhibits cell viability/proliferation, glycolysis, and colony forming activity of human medulloblastoma cells. Int J Oncol 52: 571-578, 2018.
APA
Chen, X., Wei, J., Li, C., Pierson, C.R., Finlay, J.L., & Lin, J. (2018). Blocking interleukin-6 signaling inhibits cell viability/proliferation, glycolysis, and colony forming activity of human medulloblastoma cells. International Journal of Oncology, 52, 571-578. https://doi.org/10.3892/ijo.2017.4211
MLA
Chen, X., Wei, J., Li, C., Pierson, C. R., Finlay, J. L., Lin, J."Blocking interleukin-6 signaling inhibits cell viability/proliferation, glycolysis, and colony forming activity of human medulloblastoma cells". International Journal of Oncology 52.2 (2018): 571-578.
Chicago
Chen, X., Wei, J., Li, C., Pierson, C. R., Finlay, J. L., Lin, J."Blocking interleukin-6 signaling inhibits cell viability/proliferation, glycolysis, and colony forming activity of human medulloblastoma cells". International Journal of Oncology 52, no. 2 (2018): 571-578. https://doi.org/10.3892/ijo.2017.4211
Copy and paste a formatted citation
x
Spandidos Publications style
Chen X, Wei J, Li C, Pierson CR, Finlay JL and Lin J: Blocking interleukin-6 signaling inhibits cell viability/proliferation, glycolysis, and colony forming activity of human medulloblastoma cells. Int J Oncol 52: 571-578, 2018.
APA
Chen, X., Wei, J., Li, C., Pierson, C.R., Finlay, J.L., & Lin, J. (2018). Blocking interleukin-6 signaling inhibits cell viability/proliferation, glycolysis, and colony forming activity of human medulloblastoma cells. International Journal of Oncology, 52, 571-578. https://doi.org/10.3892/ijo.2017.4211
MLA
Chen, X., Wei, J., Li, C., Pierson, C. R., Finlay, J. L., Lin, J."Blocking interleukin-6 signaling inhibits cell viability/proliferation, glycolysis, and colony forming activity of human medulloblastoma cells". International Journal of Oncology 52.2 (2018): 571-578.
Chicago
Chen, X., Wei, J., Li, C., Pierson, C. R., Finlay, J. L., Lin, J."Blocking interleukin-6 signaling inhibits cell viability/proliferation, glycolysis, and colony forming activity of human medulloblastoma cells". International Journal of Oncology 52, no. 2 (2018): 571-578. https://doi.org/10.3892/ijo.2017.4211
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