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Article

Role of nestin expression in angiogenesis and breast cancer progression

  • Authors:
    • Aleksandra Nowak
    • Jędrzej Grzegrzółka
    • Alicja Kmiecik
    • Aleksandra Piotrowska
    • Rafał Matkowski
    • Piotr Dzięgiel
  • View Affiliations / Copyright

    Affiliations: Department of Histology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland, Breast Unit, Department of Surgical Oncology, Lower Silesian Oncology Centre, 51-612 Wroclaw, Poland
  • Pages: 527-535
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    Published online on: December 11, 2017
       https://doi.org/10.3892/ijo.2017.4223
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Abstract

Nestin is an intermediate filament protein and a stem cell marker expressed in several tumours. There is growing evidence of an association between the expression level of nestin and the pathogenesis of triple-negative breast cancer (TNBC). Nestin is also expressed in newly forming tumour vessels and is a valuable marker of ongoing angiogenesis. In this study, we aimed to evaluate the prognostic value of nestin expression in breast tumour cells and to determine whether this expression influences angiogenesis. Immunohistochemical (IHC) analyses were carried out on 124 cases of invasive ductal carcinoma (IDC) of the breast with a panel of murine monoclonal antibodies against nestin, CD31, CD34, SOX-18 and Ki‑67. We evaluated nestin expression in tumour and endothelial cells, Ki‑67 in tumour cells, and CD31, CD34 and SOX-18 in endothelial cells. Our results demonstrated that nestin expression in tumour cells correlated with the area and number of vessels expressing nestin, CD31, CD34 and SOX-18. We also found a positive correlation between nestin-expressing vessels and SOX-18-expressing vessels. Our results are consistent with those of previous studies, in which nestin expression in endothelial cells was shown to be strongly associated with triple-negative subtype, poorly differentiated G3 tumours, a higher proliferation index and a shorter overall survival. Nestin expression was also examined in human breast cancer cell lines (MCF-7, SK-BR-3, MDA‑MB‑231 and BO2 cells) representing a different level of tumour aggressiveness and reflecting histological grade. A higher nestin protein level was observed in more aggressive MDA‑MB‑231 and BO2 cells than in MCF-7 and SK-BR-3 cells.
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Copy and paste a formatted citation
Spandidos Publications style
Nowak A, Grzegrzółka J, Kmiecik A, Piotrowska A, Matkowski R and Dzięgiel P: Role of nestin expression in angiogenesis and breast cancer progression. Int J Oncol 52: 527-535, 2018.
APA
Nowak, A., Grzegrzółka, J., Kmiecik, A., Piotrowska, A., Matkowski, R., & Dzięgiel, P. (2018). Role of nestin expression in angiogenesis and breast cancer progression. International Journal of Oncology, 52, 527-535. https://doi.org/10.3892/ijo.2017.4223
MLA
Nowak, A., Grzegrzółka, J., Kmiecik, A., Piotrowska, A., Matkowski, R., Dzięgiel, P."Role of nestin expression in angiogenesis and breast cancer progression". International Journal of Oncology 52.2 (2018): 527-535.
Chicago
Nowak, A., Grzegrzółka, J., Kmiecik, A., Piotrowska, A., Matkowski, R., Dzięgiel, P."Role of nestin expression in angiogenesis and breast cancer progression". International Journal of Oncology 52, no. 2 (2018): 527-535. https://doi.org/10.3892/ijo.2017.4223
Copy and paste a formatted citation
x
Spandidos Publications style
Nowak A, Grzegrzółka J, Kmiecik A, Piotrowska A, Matkowski R and Dzięgiel P: Role of nestin expression in angiogenesis and breast cancer progression. Int J Oncol 52: 527-535, 2018.
APA
Nowak, A., Grzegrzółka, J., Kmiecik, A., Piotrowska, A., Matkowski, R., & Dzięgiel, P. (2018). Role of nestin expression in angiogenesis and breast cancer progression. International Journal of Oncology, 52, 527-535. https://doi.org/10.3892/ijo.2017.4223
MLA
Nowak, A., Grzegrzółka, J., Kmiecik, A., Piotrowska, A., Matkowski, R., Dzięgiel, P."Role of nestin expression in angiogenesis and breast cancer progression". International Journal of Oncology 52.2 (2018): 527-535.
Chicago
Nowak, A., Grzegrzółka, J., Kmiecik, A., Piotrowska, A., Matkowski, R., Dzięgiel, P."Role of nestin expression in angiogenesis and breast cancer progression". International Journal of Oncology 52, no. 2 (2018): 527-535. https://doi.org/10.3892/ijo.2017.4223
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